BUB1 (NM_001278616) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BUB1A; BUB1L; hBUB1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239930 representing NM_001278616 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACCCCGGAAAATGTCCTTCAATACATACAGTGGGTAGAAGAGAATTTTCCTGAGAATAAAGAAT ACTTGATAACTTTACTAGAACATTTAATGAAGGAATTTTTAGATAAGAAGAAATACCACAATGACCCAAG ATTCATCAGTTATTGTTTAAAATTTGCTGAGTACAACAGTGACCTCCATCAATTTTTTGAGTTTCTGTAC AACCATGGGATTGGAACCCTGTCATCCCCTCTGTACATTGCCTGGGCGGGGCATCTGGAAGCCCAAGGAG AGCTGCAGCATGCCAGTGCTGTCCTTCAGAGAGGAATTCAAAACCAGGCTGAACCCAGAGAGTTCCTGCA ACAACAATACAGGTTATTTCAGACACGCCTCACTGAAACCCATTTGCCAGCTCAAGCTAGAACCTCAGAA CCTCTGCATAATGTTCAGGTTTTAAATCAAATGATAACATCAAAATCAAATCCAGGAAATAACATGGCCT GCATTTCTAAGAATCAGGGTTCAGAGCTTTCTGGAGTGATATCTTCAGCTTGTGATAAAGAGTCAAATAT GGAACGAAGAGTGATCACGATTTCTAAATCAGAATATTCTGTGCACTCATCTTTGGCATCCAAAGTTGAT GTTGAGCAGGTTGTTATGTATTGCAAGGAGAAGCTTATTCGTGGGGAATCAGAATTTTCCTTTGAAGAAT TGAGAGCCCAGAAATACAATCAACGGAGAAAGCATGAGCAATGGGTAAATGAAGACAGACATTATATGAA AAGGAAAGAAGCAAATGCTTTTGAAGAACAGCTATTAAAACAGAAAATGGATGAACTTCATAAGAAGTTG CATCAGGTGGTGGAGACATCCCATGAGGATCTGCCCGCTTCCCAGGAAAGGTCCGAGGTTAATCCAGCAC GTATGGGGCCAAGTGTAGGCTCCCAGCAGGAACTGAGAGCGCCATGTCTTCCAGTAACCTATCAGCAGAC ACCAGTGAACATGGAAAAGAACCCAAGAGAGGCACCTCCTGTTGTTCCTCCTTTGGCAAATGCTATTTCT GCAGCTTTGGTGTCCCCAGCCACCAGCCAGAGCATTGCTCCTCCTGTTCCTTTGAAAGCCCAGACAGTAA CAGACTCCATGTTTGCAGTGGCCAGCAAAGATGCTGGATGTGTGAATAAGAGTACTCATGAATTCAAGCC ACAGAGTGGAGCAGAGATCAAAGAAGGGTGTGAAACACATAAGGTTGCCAACACAAGTTCTTTTCACACA ACTCCAAACACATCACTGGGAATGGTTCAGGCAACGCCATCCAAAGTGCAGCCATCACCCACCGTGCACA CAAAAGAAGCATTAGGTTTCATCATGAATATGTTTCAGGCTCCTACACTTCCTGATATTTCTGATGACAA AGATGAATGGCAATCTCTAGATCAAAATGAAGATGCATTTGAAGCCCAGTTTCAAAAAAATGTAAGGTCA TCTGGGGCTTGGGGAGTCAATAAGATCATCTCTTCTTTGTCATCTGCTTTTCATGTGTTTGAAGATGGAA ACAAAGAAAATTATGGATTACCACAGCCTAAAAATAAACCCACAGGAGCCAGGACCTTTGGAGAACGCTC TGTCAGCAGACTTCCTTCAAAACCAAAGGAGGAAGTGCCTCATGCTGAAGAGTTTTTGGATGACTCAACT GTATGGGGTATTCGCTGCAACAAAACCCTGGCACCCAGTCCTAAGAGCCCAGGAGACTTCACATCTGCTG CACAACTTGCGTCTACACCATTCCACAAGCTTCCAGTGGAGTCAGTGCACATTTTAGAAGATAAAGAAAA TGTGGTAGCAAAACAGTGTACCCAGGCGACTTTGGATTCTTGTGAGGAAAACATGGTGGTGCCTTCAAGG GATGGAAAATTCAGTCCAATTCAAGAGAAAAGCCCAAAACAGGCCTTGTCGTCTCACATGTATTCAGCAT CCTTACTTCGTCTGAGCCAGCCTGCTGCAGGTGGGGTACTTACCTGTGAGGCAGAGTTGGGCGTTGAGGC TTGCAGACTCACAGACACTGACGCTGCCATTGCAGAAGATCCACCAGATGCTATTGCTGGGCTCCAAGCA GAATGGATGCAGATGAGTTCACTTGGGACTGTTGATGCTCCAAACTTCATTGTTGGGAACCCATGGGATG ATAAGCTGATTTTCAAACTTTTATCTGGGCTTTCTAAACCAGTGAGTTCCTATCCAAATACTTTTGAATG GCAATGTAAACTTCCAGCCATCAAGCCCAAGACTGAATTTCAATTGGGTTCTAAGCTGGTCTATGTCCAT CACCTTCTTGGAGAAGGAGCCTTTGCCCAGGTGTACGAAGCTACCCAGGGAGATCTGAATGATGCTAAAA ATAAACAGAAATTTGTTTTAAAGGTCCAAAAGCCTGCCAACCCCTGGGAATTCTACATTGGGACCCAGTT GATGGAAAGACTAAAGCCATCTATGCAGCACATGTTTATGAAGTTCTATTCTGCCCACTTATTCCAGAAT GGCAGTGTATTAGTAGGAGAGCTCTACAGCTATGGAACATTATTAAATGCCATTAACCTCTATAAAAATA CCCCTGAAAAAGTGATGCCTCAAGGTCTTGTCATCTCTTTTGCTATGAGAATGCTTTACATGATTGAGCA AGTGCATGACTGTGAAATCATTCATGGAGACATTAAACCAGACAATTTCATACTTGGAAACGGATTTTTG GAACAGGATGATGAAGATGATTTATCTGCTGGCTTGGCACTGATTGACCTGGGTCAGAGTATAGATATGA AACTTTTTCCAAAAGGAACTATATTCACAGCAAAGTGTGAAACATCTGGTTTTCAGTGTGTTGAGATGCT CAGCAACAAACCATGGAACTACCAGATCGATTACTTTGGGGTTGCTGCAACAGTATATTGCATGCTCTTT GGCACTTACATGAAAGTGAAAAATGAAGGAGGAGAGTGTAAGCCTGAAGGTCTTTTTAGAAGGCTTCCTC ATTTGGATATGTGGAATGAATTTTTTCATGTTATGTTGAATATTCCAGATTGTCATCATCTTCCATCTTT GGATTTGTTAAGGCAAAAGCTGAAGAAAGTATTTCAACAACACTATACTAACAAGATTAGGGCCCTACGT AATAGGCTAATTGTACTGCTCTTAGAATGTAAGCGTTCACGAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239930 representing NM_001278616 Red=Cloning site Green=Tags(s) MDTPENVLQYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLHQFFEFLY NHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQYRLFQTRLTETHLPAQARTSE PLHNVQVLNQMITSKSNPGNNMACISKNQGSELSGVISSACDKESNMERRVITISKSEYSVHSSLASKVD VEQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKQKMDELHKKL HQVVETSHEDLPASQERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVNMEKNPREAPPVVPPLANAIS AALVSPATSQSIAPPVPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSGAEIKEGCETHKVANTSSFHT TPNTSLGMVQATPSKVQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEWQSLDQNEDAFEAQFQKNVRS SGAWGVNKIISSLSSAFHVFEDGNKENYGLPQPKNKPTGARTFGERSVSRLPSKPKEEVPHAEEFLDDST VWGIRCNKTLAPSPKSPGDFTSAAQLASTPFHKLPVESVHILEDKENVVAKQCTQATLDSCEENMVVPSR DGKFSPIQEKSPKQALSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRLTDTDAAIAEDPPDAIAGLQA EWMQMSSLGTVDAPNFIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCKLPAIKPKTEFQLGSKLVYVH HLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFMKFYSAHLFQN GSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHDCEIIHGDIKPDNFILGNGFL EQDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLF GTYMKVKNEGGECKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALR NRLIVLLLECKRSRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278616
ORF Size	3195 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278616.1, NP_001265545.1
RefSeq Size	3576 bp
RefSeq ORF	3198 bp
Locus ID	699
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
MW	120.5 kDa
Gene Summary	This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

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