AASDH (NM_001286668) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ACSF4; LYS2; NRPS998; NRPS1098
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239866 representing NM_001286668 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAAATGTAATCTAAAGTATATCCTTGTTGAAAAAAAACAAATTAATAAATTTAAATCTTTTCATG AAACATTATTGAACTATGATACATTTACAGTGGAACATAATGACCTAGTGCTCTTCAGACTTCACTGGAA AAATACTGAGGTGAACTTGATGCTAAATGATGGAAAAGAGAAATATGAAAAAGAAAAAATAAAAAGCATA AGTTCTGAGCATGTCAATGAAGAAAAAGCAGAAGAACACATGGATCTGAGGCTAAAGCATTGCTTAGCCT ATGTTCTACATACATCAGGGACTACAGGGATACCGAAGATTGTCAGAGTGCCTCATAAGTGTATAGTACC AAATATCCAGCATTTTCGGGTACTTTTTGACATCACACAAGAAGATGTTTTGTTTCTGGCTTCACCTCTG ACCTTCGATCCTTCTGTTGTGGAAATATTTCTTGCTCTATCAAGTGGTGCCTCTCTGCTTATTGTACCAA CTTCCGTCAAGTTGCTCCCATCAAAATTAGCCAGCGTTCTCTTTTCCCATCATAGAGTGACTGTTTTGCA GGCAACACCAACATTGCTTAGAAGATTTGGATCTCAGCTTATCAAGTCAACTGTTTTGTCAGCCACTACT TCTCTTCGAGTATTAGCCCTTGGTGGTGAAGCGTTTCCATCATTGACAGTTCTCAGAAGCTGGAGAGGAG AAGGCAATAAAACACAAATATTTAATGTTTATGGTATCACAGAGGTATCAAGTTGGGCGACCATTTATAG GATTCCAGAGAAGACTCTTAACTCTACTCTCAAATGTGAATTGCCTGTACAACTGGGATTTCCACTTCTT GGAACAGTAGTTGAAGTCAGAGATACTAATGGCTTCACAATTCAGGAAGGCAGTGGCCAAGTATTTTTAG GTGGCAGAAACAGAGTGTGTTTTCTTGATGATGAAGTGACAGTACCACTTGGCACAATGCGAGCTACAGG AGACTTTGTGACTGTGAAAGATGGAGAGATTTTTTTTTTGGGACGAAAAGACAGTCAGATCAAACGTCAT GGCAAACGTCTTAACATTGAACTTGTGCAACAGGTTGCTGAAGAGCTTCAGCAAGTGGAGTCTTGTGCAG TTACATGGTATAATCAGGAAAAATTAATTCTCTTCATGGTGTCTAAAGATGCTTCAGTAAAAGAATACAT CTTTAAAGAACTGCAGAAATATCTTCCAAGTCATGCAGTCCCGGATGAGCTTGTATTGATCGACTCTCTA CCATTTACATCCCACGGCAAAATTGATGTTTCTGAGTTAAACAAGATATATTTAAACTACATAAACTTGA AGTCTGAGAATAAGCTCAGTGGGAAAGAGGACCTTTGGGAAAAATTACAGTATTTGTGGAAGTCTACTCT GAATCTCCCAGAAGATCTTTTGAGGGTTCCTGATGAGTCACTCTTCTTAAATAGTGGTGGAGATTCCTTA AAGTCCATCCGGCTCCTCAGTGAGATTGAAAAACTTGTTGGTACATCAGTACCTGGGCTTCTGGAAATTA TTCTCAGCAGTTCCATTTTAGAGATTTATAATCACATCCTTCAAACAGTGGTTCCAGATGAAGATGTGAC ATTCAGGAAGAGTTGTGCCACAAAAAGGAAACTCAGCGACATTAATCAAGAGGAAGCCAGTGGAACATCT TTACATCAGAAAGCCATCATGACTTTCACTTGCCACAATGAGATTAATGCTTTTGTTGTACTGAGCAGAG GGAGTCAAATTTTGTCTCTGAATTCCACTAGGTTTTTAACAAAGTTAGGACATTGCTCTTCAGCCTGTCC TTCTGACTCAGTTTCACAGACCAACATTCAAAATTTGAAAGGCTTAAATTCTCCAGTTCTTATTGGGAAG TCAAAAGATCCATCCTGTGTTGCAAAAGTTTCTGAAGAGGGGAAACCTGCGATAGGGACTCAGAAAATGG AGTTACATGTGAGGTGGAGGTCAGACACAGGCAAATGTGTAGATGCTTCACCGCTGGTTGTAATACCCAC TTTTGATAAGTCATCTACAACTGTGTACATTGGTTCCCATTCTCATAGAATGAAGGCAGTTGACTTTTAC TCTGGGAAGGTAAAATGGGAACAGATTTTGGGAGATCGAATTGAATCCTCAGCATGTGTATCTAAGTGTG GAAACTTTATTGTGGTGGGCTGTTATAATGGATTAGTTTATGTTCTGAAAAGTAATAGTGGAGAAAAATA CTGGATGTTTACTACTGAAGATGCTGTCAAAAGCTCGGCAACCATGGATCCAACCACAGGACTCATTTAC ATTGGATCTCATGACCAGCACGCATATGCTTTAGATATTTATAGAAAGAAGTGTGTTTGGAAGTCAAAAT GTGGAGGAACTGTCTTTTCCTCTCCGTGTTTGAACCTGATTCCACATCATTTGTATTTTGCTACATTGGG AGGACTTTTACTGGCTGTAAATCCTGCTACTGGGAACGTTATTTGGAAACATTCCTGTGGAAAACCACTC TTCTCTTCCCCACAATGTTGCTCACAGTATATTTGTATTGGCTGTGTAGATGGGAATTTACTCTGCTTTA CTCACTTTGGAGAACAGGTTTGGCAGTTCTCTACCAGTGGACCAATCTTTTCATCCCCGTGTACCTCACC ATCAGAGCAAAAAATATTTTTTGGTTCCCATGATTGCTTTATCTACTGTTGTAACATGAAAGGTCACCTG CAGTGGAAATTTGAAACTACTTCAAGGGTCTATGCAACACCGTTTGCTTTCCATAACTACAATGGCAGCA ATGAAATGTTGCTGGCAGCAGCATCTACTGATGGGAAAGTGTGGATCTTGGAATCTCAGAGTGGACAATT GCAAAGTGTTTATGAACTTCCTGGAGAAGTCTTCTCTTCTCCTGTGGTCCTGGAATCAATGCTCATTATT GGGTGTAGAGATAATTATGTTTATTGTCTGGATTTATTGGGTGGCAATCAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239866 representing NM_001286668 Red=Cloning site Green=Tags(s) MKKCNLKYILVEKKQINKFKSFHETLLNYDTFTVEHNDLVLFRLHWKNTEVNLMLNDGKEKYEKEKIKSI SSEHVNEEKAEEHMDLRLKHCLAYVLHTSGTTGIPKIVRVPHKCIVPNIQHFRVLFDITQEDVLFLASPL TFDPSVVEIFLALSSGASLLIVPTSVKLLPSKLASVLFSHHRVTVLQATPTLLRRFGSQLIKSTVLSATT SLRVLALGGEAFPSLTVLRSWRGEGNKTQIFNVYGITEVSSWATIYRIPEKTLNSTLKCELPVQLGFPLL GTVVEVRDTNGFTIQEGSGQVFLGGRNRVCFLDDEVTVPLGTMRATGDFVTVKDGEIFFLGRKDSQIKRH GKRLNIELVQQVAEELQQVESCAVTWYNQEKLILFMVSKDASVKEYIFKELQKYLPSHAVPDELVLIDSL PFTSHGKIDVSELNKIYLNYINLKSENKLSGKEDLWEKLQYLWKSTLNLPEDLLRVPDESLFLNSGGDSL KSIRLLSEIEKLVGTSVPGLLEIILSSSILEIYNHILQTVVPDEDVTFRKSCATKRKLSDINQEEASGTS LHQKAIMTFTCHNEINAFVVLSRGSQILSLNSTRFLTKLGHCSSACPSDSVSQTNIQNLKGLNSPVLIGK SKDPSCVAKVSEEGKPAIGTQKMELHVRWRSDTGKCVDASPLVVIPTFDKSSTTVYIGSHSHRMKAVDFY SGKVKWEQILGDRIESSACVSKCGNFIVVGCYNGLVYVLKSNSGEKYWMFTTEDAVKSSATMDPTTGLIY IGSHDQHAYALDIYRKKCVWKSKCGGTVFSSPCLNLIPHHLYFATLGGLLLAVNPATGNVIWKHSCGKPL FSSPQCCSQYICIGCVDGNLLCFTHFGEQVWQFSTSGPIFSSPCTSPSEQKIFFGSHDCFIYCCNMKGHL QWKFETTSRVYATPFAFHNYNGSNEMLLAAASTDGKVWILESQSGQLQSVYELPGEVFSSPVVLESMLII GCRDNYVYCLDLLGGNQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286668
ORF Size	2994 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286668.2
RefSeq Size	3342 bp
RefSeq ORF	2997 bp
Locus ID	132949
UniProt ID	Q4L235
Protein Pathways	Lysine biosynthesis, Lysine degradation, Metabolic pathways
MW	111.9 kDa
Gene Summary	This gene encodes a member of the non-ribosome peptide syntesase (NRPS) enzyme family. The encoded protein contains an AMP-binding domain, PP-binding (phosphopantetheine, or pantetheine 4'phosphate-binding) domain and the Pyrrolo-quinoline quinon (PQQ) binding domain. The protein is expressed in several adult tissues. [provided by RefSeq, Apr 2016]

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