EPB41L3 (NM_001281533) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4.1B; DAL-1; DAL1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239731 representing NM_001281533 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGACCGAATCTGGATCAGACTCGGAATCCAAGCCGGACCAGGAGGCCGAGCCCCAGGAGGCGGCGG GGGCGCAGGGGCGCGCGGGGGCGCCCGTGCCGGAGCCGCCCAAGGAGGAGCAGCAGCAGGCCCTGGAGCA GTTCGCCGCCGCTGCAGCGCACAGCACCCCGGTGCGGAGGGAGGTCACTGACAAGGAACAGGAGTTTGCT GCCAGGGCTGCAAAACAGCTCGAATATCAGCAATTAGAAGACGATAAACTTTCTCAGAAATCATCTAGCA GTAAACTCTCTCGGTCTCCATTAAAGATTGTCAAAAAGCCTAAAAGCATGCAGTGCAAAGTGATACTTCT CGATGGATCAGAATATACCTGTGATGTAGAGAAACGCTCCAGAGGACAAGTGCTGTTTGATAAAGTGTGT GAACACTTGAACTTGCTAGAGAAAGACTACTTTGGGCTTACGTATCGAGATGCTGAAAACCAGAAGAATT GGTTGGACCCTGCTAAGGAAATAAAAAAACAGGTTCGAAGTGGTGCTTGGCACTTTTCATTTAATGTGAA ATTTTATCCACCAGACCCTGCCCAACTATCTGAAGATATCACCAGGTACTACCTCTGCTTGCAGTTGCGA GATGACATCGTGTCCGGAAGGCTGCCCTGCTCCTTTGTTACCCTGGCCTTGCTGGGCTCCTACACTGTCC AGTCAGAGCTCGGAGACTATGACCCAGATGAATGTGGGAGCGATTACATTAGTGAGTTCCGCTTTGCACC AAACCACACTAAAGAACTGGAAGACAAAGTGATCGAGCTGCACAAGAGCCACAGAGGAATGACGCCAGCA GAAGCAGAGATGCATTTCTTGGAAAATGCCAAAAAATTATCAATGTATGGGGTAGATTTACATCATGCTA AGGACTCAGAAGGGGTAGAAATTATGTTAGGAGTTTGTGCAAGTGGTCTGTTGATATATCGCGACCGGCT GCGAATAAACAGATTTGCCTGGCCCAAGGTTCTAAAGATTTCATACAAACGGAACAACTTTTACATTAAG ATCCGGCCGGGAGAGTTTGAACAATTTGAAAGCACCATTGGGTTTAAGCTGCCAAACCATCGAGCTGCCA AGCGTTTATGGAAAGTATGTGTTGAGCATCATACATTTTTCAGACTACTGTTACCAGAAGCACCTCCCAA GAAATTCCTAACCTTGGGTTCCAAGTTTCGTTATAGTGGCAGGACACAAGCGCAAACGAGAAGAGCCAGT GCGTTGATAGATCGCCCAGCACCTTACTTTGAACGCTCATCCAGCAAACGTTATACCATGTCTCGCAGCT TGGATGGAGCATCAGTGAATGAAAACCATGAAATATACATGAAGGATTCTATGTCTGCTGCAGAGGTTGG TACTGGCCAGTACGCCACAACAAAAGGCATCTCTCAGACCAACTTGATCACCACTGTGACTCCGGAGAAG AAGGCTGAGGAGGAGCGGGACGAGGAAGAGGACAAACGGAGGAAGGGGGAAGAAGTCACGCCCATCTCGG CCATCCGGCACGAGGGAAAGACTGACAGTGAGCGCACGGACACCGCAGCCGACGGGGAGACCACTGCCAC TGAGTCGGACCAGGAGGAAGATGCAGAGCTCAAGGCACAGGAGCTAGAAAAAACTCAAGATGACCTGATG AAACATCAAACCAACATTAGCGAGCTGAAAAGAACCTTCTTAGAAACCTCAACAGACACTGCCGTAACGA ATGAATGGGAGAAGAGGCTTTCCACCTCCCCCGTGCGACTGGCCGCCAGGCAGGAGGATGCCCCCATGAT CGAACCACTTGTCCCTGAAGAGACTAAGCAGTCTTCTGGGGAAAAGCTCATGGATGGCTCTGAAATCTTC AGTTTATTAGAGTCTGCGCGAAAACCAACAGAATTCATAGGAGGGGTTACTTCTACTTCTCAAAGCTGGG TTCAGAAAATGGAAACCAAGACGGAGTCCAGTGGAATAGAGACGGAACCCACCGTGCACCACCTGCCGCT TAGCACTGAGAAGGTGGTGCAGGAGACCGTGTTGGTGGAGGAGCGGCGTGTGGTGCACGCGAGTGGGGAT GCTTCTTACTCGGCGGGAGACAGCGGGGATGCTGCAGCACAGCCCGCATTCACAGGCATTAAAGGGAAAG AGGGCTCTGCCTTGACGGAGGGGGCTAAAGAGGAAGGAGGGGAGGAGGTCGCTAAAGCTGTCCTGGAACA GGAAGAGACAGCCGCTGCTTCCCGTGAGCGACAAGAGGAGCAGAGTGCAGCCATCCACATTTCAGAAACT TTGGAACAAAAACCTCATTTTGAGTCCTCAACGGTGAAGACGGAAACCATCAGTTTTGGCAGTGTTTCAC CGGGAGGAGTAAAGCTAGAAATTTCCACGAAGGAAGTGCCAGTAGTTCACACCGAAACCAAAACCATCAC ATATGAATCATCACAGGTCGATCCAGGCACAGATCTGGAGCCAGGCGTGCTGATGAGTGCACAGACGATC ACATCTGAAACCACCAGTACCACCACCACTACGCACATCACCAAAACTGTGAAAGGGGGCATTTCAGAGA CAAGAATTGAGAAGCGAATAGTCATCACGGGGGATGCAGACATTGACCATGACCAGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239731 representing NM_001281533 Red=Cloning site Green=Tags(s) MTTESGSDSESKPDQEAEPQEAAGAQGRAGAPVPEPPKEEQQQALEQFAAAAAHSTPVRREVTDKEQEFA ARAAKQLEYQQLEDDKLSQKSSSSKLSRSPLKIVKKPKSMQCKVILLDGSEYTCDVEKRSRGQVLFDKVC EHLNLLEKDYFGLTYRDAENQKNWLDPAKEIKKQVRSGAWHFSFNVKFYPPDPAQLSEDITRYYLCLQLR DDIVSGRLPCSFVTLALLGSYTVQSELGDYDPDECGSDYISEFRFAPNHTKELEDKVIELHKSHRGMTPA EAEMHFLENAKKLSMYGVDLHHAKDSEGVEIMLGVCASGLLIYRDRLRINRFAWPKVLKISYKRNNFYIK IRPGEFEQFESTIGFKLPNHRAAKRLWKVCVEHHTFFRLLLPEAPPKKFLTLGSKFRYSGRTQAQTRRAS ALIDRPAPYFERSSSKRYTMSRSLDGASVNENHEIYMKDSMSAAEVGTGQYATTKGISQTNLITTVTPEK KAEEERDEEEDKRRKGEEVTPISAIRHEGKTDSERTDTAADGETTATESDQEEDAELKAQELEKTQDDLM KHQTNISELKRTFLETSTDTAVTNEWEKRLSTSPVRLAARQEDAPMIEPLVPEETKQSSGEKLMDGSEIF SLLESARKPTEFIGGVTSTSQSWVQKMETKTESSGIETEPTVHHLPLSTEKVVQETVLVEERRVVHASGD ASYSAGDSGDAAAQPAFTGIKGKEGSALTEGAKEEGGEEVAKAVLEQEETAAASRERQEEQSAAIHISET LEQKPHFESSTVKTETISFGSVSPGGVKLEISTKEVPVVHTETKTITYESSQVDPGTDLEPGVLMSAQTI TSETTSTTTTTHITKTVKGGISETRIEKRIVITGDADIDHDQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001281533
ORF Size	2649 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281533.1, NP_001268462.1
RefSeq Size	3972 bp
RefSeq ORF	2652 bp
Locus ID	23136
UniProt ID	Q9Y2J2
Protein Pathways	Tight junction
MW	98.9 kDa
Gene Summary	Tumor suppressor that inhibits cell proliferation and promotes apoptosis. Modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5.[UniProtKB/Swiss-Prot Function]

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