CDC2L1 (CDK11B) (NM_001291345) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CDC2L1; CDK11; CDK11-p46; CDK11-p58; CDK11-p110; CLK-1; p58; p58CDC2L1; p58CLK-1; PITSLREA; PK58
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239535 representing NM_001291345 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGA AGGAACAAGAGGAGAAAGCAGAGATAAAACGCTTAAAAAATTCTGATGACCGGGATTCCAAGCGGGATTC CCTTGAGGAGGGGGAGCTGAGAGATCACCGCATGGAGATCACAATAAGGAACTCCCCGTATAGAAGAGAA GACTCTATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGA AAGAAAAAGCTCATCACAGAAAAGATGAAAAGAGAAAAGAGAAACGGAAGCATGCTAGAGTGAAAGAAAA AGAAAGAGAGCACGAACGTCGGAAACGGCATCGAGAAGAACAGGATAAAGCTCGCCGGGAATGGGAAAGA CAGAAGAGAAGGGAGATGGCAAGGGAGCATTCCAGGAGAGAAAGGGACCGCTTGGAGCAGTTAGAAAGGA AGCGGGAGCGGGAGCGCAAGATGCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCG GGCAGAGGAGCGGCGCAAGGAGCGGGAGGCCCGCAGGGAAGTGTCTGCACATCACCGAACGATGAGAGAG GACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAGCCCGCCTCGGCCGCCGCGGGAGCGGTTCG AGTTGGGAGACGGCCGGAAGCCAGTAAAAGAAGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACA GGACATCAGCGACAGCGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGAATCAGGCTCAGGTTCT GAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGGAGCACCAGTGAAGAATCAGAGGAGGAGG AGGAGGAAGAGGAAGAGGAGGAGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGCCGA AGAAGTAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCA GAGTCACGGTTCGACCGAGATTCCGGGGAGAGTGAAGAAGCAGAGGAAGAAGTGGGTGAGGGAACGCCGC AGAGCAGCGCCCTGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGTCGCCCATCGAGCTCAAGCA GGAGCTGCCCAAGTACCTGCCGGCCCTGCAGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGG ATCGAGGAGGGCACCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAA AGCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCGCTGAGGGAGATCAACACCATCCT CAAGGCCCAGCATCCCAACATCGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTAC ATCGTGATGAACTATGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAG GGGAGGTGAAGACCCTGATGATCCAGCTGCTGCGTGGGGTGAAACACCTGCACGACAACTGGATCCTGCA CCGTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGACTTCGGGCTG GCGCGGGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACCCTGTGGTACCGCGCCCCAG AGCTGCTGCTTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGTCAGTGGGTTGCATCTTCGGGGA GCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAAGTCAGAAATCGATCAGATCAACAAGGTGTTCAAGGAT CTGGGGACCCCTAGTGAGAAAATCTGGCCCGGCTACAGCGAGCTCCCAGCAGTCAAGAAGATGACCTTCA GCGAGCACCCCTACAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCAT GAACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATTTC CGCGAGACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTG TGAAGCGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCT GAAGGAGACGGGCTTCCACCTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTC AAGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239535 representing NM_001291345 Red=Cloning site Green=Tags(s) MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKRDSLEEGELRDHRMEITIRNSPYRRE DSMEDRGEEDDSLAIKPPQQMSRKEKAHHRKDEKRKEKRKHARVKEKEREHERRKRHREEQDKARREWER QKRREMAREHSRRERDRLEQLERKRERERKMREQQKEQREQKERERRAEERRKEREARREVSAHHRTMRE DYSDKVKASHWSRSPPRPPRERFELGDGRKPVKEEKMEERDLLSDLQDISDSERKTSSAESSSAESGSGS EEEEEEEEEEEEEGSTSEESEEEEEEEEEEEEETGSNSEEASEQSAEEVSEEEMSEDEERENENHLLVVP ESRFDRDSGESEEAEEEVGEGTPQSSALTEGDYVPDSPALSPIELKQELPKYLPALQGCRSVEEFQCLNR IEEGTYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLREINTILKAQHPNIVTVREIVVGSNMDKIY IVMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLLRGVKHLHDNWILHRDLKTSNLLLSHAGILKVGDFGL AREYGSPLKAYTPVVVTLWYRAPELLLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGKSEIDQINKVFKD LGTPSEKIWPGYSELPAVKKMTFSEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRISAEDGLKHEYF RETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGASAAGPGFSL KF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291345
ORF Size	2316 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291345.2
RefSeq Size	3072 bp
RefSeq ORF	2319 bp
Locus ID	984
UniProt ID	P21127
Protein Families	Druggable Genome, Transcription Factors
MW	90.7 kDa
Gene Summary	This gene encodes a member of the serine/threonine protein kinase family. Members of this kinase family are known to be essential for eukaryotic cell cycle control. Due to a segmental duplication, this gene shares very high sequence identity with a neighboring gene. These two genes are frequently deleted or altered in neuroblastoma. The protein kinase encoded by this gene can be cleaved by caspases and may play a role in cell apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

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