MSK2 / RSK-B (RPS6KA4) (NM_001300802) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MSK2; RSK-B; S6K-alpha-4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239520 representing NM_001300802 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACGAGGACGACGATGAGAGCTGCGCCGTGGAGCTGCGGATCACAGAAGCCAACCTGACCGGGC ACGAGGAGAAGGTGAGCGTGGAGAACTTCGAGCTGCTCAAGGTGCTGGGCACGGGAGCCTACGGCAAGGT GTTCCTGGTGCGGAAGGCGGGCGGGCACGACGCGGGGAAGCTGTACGCCATGAAGGTGCTGCGCAAGGCG GCGCTGGTGCAGCGCGCCAAGACGCAAGAGCACACGCGCACCGAGCGCTCGGTGCTGGAGCTGGTGCGCC AGGCGCCCTTCCTGGTCACGCTGCACTACGCTTTCCAGACGGATGCCAAGCTGCACCTCATCCTGGACTA TGTGAGCGGCGGGGAGATGTTCACCCACCTCTACCAGCGCCAGTACTTCAAGGAGGCTGAGGTGCGCGTG TATGGGGGTGAGATCGTGCTGGCCCTGGAACACCTGCACAAGCTCGGCATCATTTACCGAGACCTGAAAC TGGAGAATGTGCTGCTGGACTCCGAGGGCCACATTGTCCTCACGGACTTCGGGCTGAGCAAGGAGTTCCT GACGGAGGAGAAAGAGCGGACCTTCTCCTTCTGTGGCACCATCGAGTACATGGCCCCCGAAATCATCCGT AGCAAGACGGGGCATGGCAAGGCTGTGGACTGGTGGAGCCTGGGCATCTTGCTCTTCGAGCTGCTGACGG GGGCCTCGCCCTTCACCCTGGAGGGCGAGAGGAACACGCAGGCTGAGGTGTCTCGACGGATCCTGAAGTG CTCCCCTCCCTTCCCCCCTCGGATCGGGCCCGTGGCGCAGGACCTGCTGCAGCGGCTGCTTTGTAAGGAT CCTAAGAAGCGATTGGGCGCGGGGCCCCAGGGGGCACAAGAAGTCCGGAACCATCCCTTCTTCCAGGGCC TCGATTGGGTGGCTCTGGCTGCCAGGAAGATTCCAGCCCCATTCCGGCCCCAAATCCGCTCAGAGCTGGA TGTGGGCAACTTTGCGGAGGAATTCACTCGGCTGGAGCCTGTCTACTCACCCCCTGGCAGCCCCCCACCT GGGGACCCCCGAATCTTTCAGGGATACTCCTTTGTGGCACCCTCCATTCTCTTTGACCACAACAACGCGG TGATGACCGATGGGCTGGAAGCGCCTGGTGCTGGAGACCGGCCAGGTCGGGCAGCGGTGGCCAGGAGCGC TATGATGCAGTACGAGCTGGACCTGCGGGAGCCTGCGCTGGGCCAGGGCAGCTTTTCTGTGTGTCGCCGC TGCCGCCAGCGCCAGAGCGGCCAGGAGTTCGCAGTCAAGATCCTCAGTCGCAGGCTGGAGGCGAACACGC AGCGCGAAGTGGCTGCCCTGCGCCTGTGCCAGTCACACCCCAACGTGGTGAATCTGCACGAGGTGCATCA CGACCAGCTGCACACGTACCTGGTCCTGGAGCTGCTGCGGGGCGGGGAGCTGCTGGAGCACATCCGCAAG AAGCGGCACTTCAGCGAGTCGGAAGCAAGCCAGATCCTGCGCAGCCTCGTGTCGGCCGTGAGCTTCATGC ACGAGGAGGCGGGCGTGGTGCACCGCGACCTCAAGCCGGAGAACATCCTGTACGCCGACGACACGCCCGG GGCCCCGGTGAAAATCATCGACTTCGGGTTCGCGCGGTTGCGGCCGCAGAGTCCCGGGGTGCCCATGCAG ACGCCCTGCTTCACGCTGCAGTACGCTGCCCCCGAGCTGCTGGCGCAGCAGGGCTACGACGAGTCCTGCG ACCTCTGGAGCCTGGGCGTCATTCTGTACATGATGCTGTCGGGGCAGGTCCCCTTCCAGGGGGCCTCTGG CCAGGGCGGGCAGAGCCAGGCGGCCGAGATCATGTGCAAAATCCGCGAGGGGCGCTTCTCCCTTGACGGG GAGGCCTGGCAGGGTGTATCCGAGGAAGCCAAGGAGCTGGTCCGAGGGCTCCTGACCGTGGACCCCGCCA AGCGGCTGAAGCTCGAGGGACTGCGGGGCAGCTCGTGGCTGCAGGACGGCAGCGCGCGCTCCTCGCCCCC GCTCCGGACGCCCGACGTGCTCGAGTCCTCTGGGCCCGCAGTGCGCTCGGGTCTCAACGCCACCTTCATG GCATTCAACCGGGGCAAGCGGGAGGGCTTCTTCCTGAAGAGCGTGGAGAATGCACCCCTGGCCAAGCGGC GGAAGCAGAAGCTGCGGAGCGCCACCGCCTCCCGCCGGGGCTCCCCTGCACCAGCCAACCCGGGCCGAGC CCCCGTCGCCTCCAAAGGGGCCCCCCGCCGAGCCAACGGCCCCCTGCCCCCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239520 representing NM_001300802 Red=Cloning site Green=Tags(s) MGDEDDDESCAVELRITEANLTGHEEKVSVENFELLKVLGTGAYGKVFLVRKAGGHDAGKLYAMKVLRKA ALVQRAKTQEHTRTERSVLELVRQAPFLVTLHYAFQTDAKLHLILDYVSGGEMFTHLYQRQYFKEAEVRV YGGEIVLALEHLHKLGIIYRDLKLENVLLDSEGHIVLTDFGLSKEFLTEEKERTFSFCGTIEYMAPEIIR SKTGHGKAVDWWSLGILLFELLTGASPFTLEGERNTQAEVSRRILKCSPPFPPRIGPVAQDLLQRLLCKD PKKRLGAGPQGAQEVRNHPFFQGLDWVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPP GDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVARSAMMQYELDLREPALGQGSFSVCRR CRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLELLRGGELLEHIRK KRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQSPGVPMQ TPCFTLQYAAPELLAQQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIREGRFSLDG EAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRSGLNATFM AFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAPVASKGAPRRANGPLPPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300802
ORF Size	2295 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300802.2
RefSeq Size	3128 bp
RefSeq ORF	2298 bp
Locus ID	8986
UniProt ID	O75676
Protein Families	Druggable Genome, Protein Kinase, Transcription Factors
Protein Pathways	MAPK signaling pathway, Neurotrophin signaling pathway
MW	85.2 kDa
Gene Summary	This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

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