IL12RB1 (NM_001290024) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CD212; IL-12R-BETA1; IL12RB; IMD30
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239356 representing NM_001290024 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCGTTGGTCTTTTCTCCTTGCTCAGCTTCAATGTGTTCCGGAGTGGGGACGGGGTGGCTGAACCTC GCAGGTGGCAGAGAGGCTCCCCTGGGGCTGTGGGGCTCTACGTGGATCCGATGGAGCCGCTGGTGACCTG GGTGGTCCCCCTCCTCTTCCTCTTCCTGCTGTCCAGGCAGGGCGCTGCCTGCAGAACCAGTGAGTGCTGT TTTCAGGACCCGCCATATCCGGATGCAGACTCAGGCTCGGCCTCGGGCCCTAGGGACCTGAGATGCTATC GGATATCCAGTGATCGTTACGAGTGCTCCTGGCAGTATGAGGGTCCCACAGCTGGGGTCAGCCACTTCCT GCGGTGTTGCCTTAGCTCCGGGCGCTGCTGCTACTTCGCCGCCGGCTCAGCCACCAGGCTGCAGTTCTCC GACCAGGCTGGGGTGTCTGTGCTGTACACTGTCACACTCTGGGTGGAATCCTGGGCCAGGAACCAGACAG AGAAGTCTCCTGAGGTGACCCTGCAGCTCTACAACTCAGTTAAATATGAGCCTCCTCTGGGAGACATCAA GGTGTCCAAGTTGGCCGGGCAGCTGCGTATGGAGTGGGAGACCCCGGATAACCAGGTTGGTGCTGAGGTG CAGTTCCGGCACCGGACACCCAGCAGCCCATGGAAGTTGGGCGACTGCGGACCTCAGGATGATGATACTG AGTCCTGCCTCTGCCCCCTGGAGATGAATGTGGCCCAGGAATTCCAGCTCCGACGACGGCAGCTGGGGAG CCAAGGAAGTTCCTGGAGCAAGTGGAGCAGCCCCGTGTGCGTTCCCCCTGAAAACCCCCCACAGCCTCAG GTGAGATTCTCGGTGGAGCAGCTGGGCCAGGATGGGAGGAGGCGGCTGACCCTGAAAGAGCAGCCAACCC AGCTGGAGCTTCCAGAAGGCTGTCAAGGGCTGGCGCCTGGCACGGAGGTCACTTACCGACTACAGCTCCA CATGCTGTCCTGCCCGTGTAAGGCCAAGGCCACCAGGACCCTGCACCTGGGGAAGATGCCCTATCTCTCG GGTGCTGCCTACAACGTGGCTGTCATCTCCTCGAACCAATTTGGTCCTGGCCTGAACCAGACGTGGCACA TTCCTGCCGACACCCACACAGAACCAGTGGCTCTGAATATCAGCGTCGGAACCAACGGGACCACCATGTA TTGGCCAGCCCGGGCTCAGAGCATGACGTATTGCATTGAATGGCAGCCTGTGGGCCAGGACGGGGGCCTT GCCACCTGCAGCCTGACTGCGCCGCAAGACCCGGATCCGGCTGGAATGGCAACCTACAGCTGGAGTCGAG AGTCTGGGGCAATGGGGCAGGAAAAGTGTTACTACATTACCATCTTTGCCTCTGCGCACCCCGAGAAGCT CACCTTGTGGTCTACGGTCCTGTCCACCTACCACTTTGGGGGCAATGCCTCAGCAGCTGGGACACCGCAC CACGTCTCGGTGAAGAATCATAGCTTGGACTCTGTGTCTGTGGACTGGGCACCATCCCTGCTGAGCACCT GTCCCGGCGTCCTAAAGGAGTATGTTGTCCGCTGCCGAGATGAAGACAGCAAACAGGTGTCAGAGCATCC CGTGCAGCCCACAGAGACCCAAGTTACCCTCAGTGGCCTGCGGGCTGGTGTAGCCTACACGGTGCAGGTG CGAGCAGACACAGCGTGGCTGAGGGGTGTCTGGAGCCAGCCCCAGCGCTTCAGCATCGAAGTGCAGGTTT CTGATTGGCTCATCTTCTTCGCCTCCCTGGGGAGCTTCCTGAGCATCCTTCTCGTGGGCGTCCTTGGCTA CCTTGGCCTGAACAGGGCCGCACGGCACCTGTGCCCGCCGCTGCCCACACCCTGTGCCAGCTCCGCCATT GAGTTCCCTGGAGGGAAGGAGACTTGGCAGTGGATCAACCCAGTGGACTTCCAGGAAGAGGCATCCCTGC AGGAGGCCCTGGTGGTAGAGATGTCCTGGGACAAAGGCGAGAGGACTGAGCCTCTCGAGAAGACAGAGCT ACCTGAGGGTGCCCCTGAGCTGGCCCTGGATACAGAGTTGTCCTTGGAGGATGGAGACAGGTGCAAGGCC AAGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239356 representing NM_001290024 Red=Cloning site Green=Tags(s) MFVGLFSLLSFNVFRSGDGVAEPRRWQRGSPGAVGLYVDPMEPLVTWVVPLLFLFLLSRQGAACRTSECC FQDPPYPDADSGSASGPRDLRCYRISSDRYECSWQYEGPTAGVSHFLRCCLSSGRCCYFAAGSATRLQFS DQAGVSVLYTVTLWVESWARNQTEKSPEVTLQLYNSVKYEPPLGDIKVSKLAGQLRMEWETPDNQVGAEV QFRHRTPSSPWKLGDCGPQDDDTESCLCPLEMNVAQEFQLRRRQLGSQGSSWSKWSSPVCVPPENPPQPQ VRFSVEQLGQDGRRRLTLKEQPTQLELPEGCQGLAPGTEVTYRLQLHMLSCPCKAKATRTLHLGKMPYLS GAAYNVAVISSNQFGPGLNQTWHIPADTHTEPVALNISVGTNGTTMYWPARAQSMTYCIEWQPVGQDGGL ATCSLTAPQDPDPAGMATYSWSRESGAMGQEKCYYITIFASAHPEKLTLWSTVLSTYHFGGNASAAGTPH HVSVKNHSLDSVSVDWAPSLLSTCPGVLKEYVVRCRDEDSKQVSEHPVQPTETQVTLSGLRAGVAYTVQV RADTAWLRGVWSQPQRFSIEVQVSDWLIFFASLGSFLSILLVGVLGYLGLNRAARHLCPPLPTPCASSAI EFPGGKETWQWINPVDFQEEASLQEALVVEMSWDKGERTEPLEKTELPEGAPELALDTELSLEDGDRCKA KM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290024
ORF Size	2106 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290024.1, NP_001276953.1
RefSeq Size	2791 bp
RefSeq ORF	2109 bp
Locus ID	3594
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
MW	77.9 kDa
Gene Summary	The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

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