FARP2 (NM_001282983) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	FIR; FRG; PLEKHC3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239195 representing NM_001282983 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAGATAGAAGGAACATACAGAGTCCTGCAGACTGCAGGGATGCGCTTGGGTGCCCAGACCCCTG TGGGAGTTAGCACCCTTGAGCCTGGGCAGACTCTCTTGCCCAGAATGCAAGAGAAGCACCTGCACCTCAG AGTAAAGCTGCTGGACAACACCATGGAAATATTTGACATTGAGCCTAAATGCGATGGCCAGGTATTACTG ACACAAGTGTGGAAGCGTTTAAACCTGGTAGAATGTGACTACTTCGGGATGGAGTTTCAAAATACTCAGT CCTACTGGATTTGGCTTGAACCTATGAAACCCATCATTAGGCAAATACGAAGGCCAAAGAATGTGGTGCT TCGCCTAGCTGTAAAATTTTTTCCACCTGATCCTGGTCAGCTACAAGAAGAATATACAAGATACTTGTTT GCCTTGCAACTTAAGAGAGACCTGCTGGAAGAGCGTTTGACCTGTGCTGACACCACAGCGGCCCTTCTCA CGTCCCATCTCCTGCAGTCGGAAATAGGAGATTACGATGAAACGCTGGACCGAGAGCACCTCAAAGTGAA CGAGTATTTGCCTGGCCAGCAGCACTGCCTTGAGAAGATACTAGAATTCCATCAGAAGCACGTGGGCCAG ACACCTGCTGAGTCGGATTTCCAGGTGCTCGAAATTGCTCGAAAGTTGGAAATGTACGGCATCAGATTTC ACATGGCTTCTGACAGGGAAGGAACCAAGATTCAACTGGCAGTTTCCCACATGGGTGTACTCGTGTTCCA GGGCACCACCAAAATCAACACTTTCAACTGGTCCAAGGTCCGTAAACTAAGCTTCAAGAGGAAAAGATTT CTTATCAAACTTCATCCAGAGGTTCATGGACCTTACCAGGACACATTAGAATTTTTGTTGGGTAGTAGAG ATGAATGTAAGAACTTCTGGAAGATTTGTGTGGAGTATCACACCTTTTTTAGACTTTTGGACCAACCTAA GCCAAAAGCAAAAGCCGTCTTCTTCAGCCGGGGCTCCTCCTTCAGATACAGTGGAAGAACTCAGAAACAA CTAGTAGATTATTTCAAAGACAGTGGAATGAAGAGAATTCCATATGAAAGAAGGCACAGCAAGACCCACA CGTCCGTTCGAGCTCTGACTGCAGACCTACCAAAACAGAGCATCTCATTCCCCGAGGGATTGAGGACTCC TGCCTCCCCATCTTCAGCGAATGCCTTTTACTCGCTCTCTCCCTCCACTCTGGTCCCCTCTGGCCTGCCA GAGTTTAAGGACAGCAGCAGCTCCCTCACAGATCCCCAGGTTTCCTACGTCAAGAGTCCAGCTGCAGAGA GGCGCAGTGGAGCAGTGGCTGGAGGCCCCGACACACCATCGGCCCAGCCCCTCGGGCCCCCCGCACTCCA GCCTGGTCCAGGCCTTTCCACGAAGAGTCCTCAGCCTTCTCCCTCCAGCCGGAAGAGCCCCCTGAGTCTG AGCCCTGCATTTCAGGTGCCTTTGGGCCCAGCTGAACAGGGCTCATCCCCACTCCTGAGCCCTGTCCTCA GTGATGCTGGCGGAGCCGGGATGGACTGCGAGGAGCCCAGACACAAGCGCGTGCCTGCAGACGAGGCCTA CTTCATAGTCAAAGAGATTCTCGCTACAGAACGAACATACCTCAAGGATTTAGAAGTTATTACCGTGTGG TTCCGCAGCGCAGTGGTGAAGGAGGACGCCATGCCTGCGACTCTGATGACGCTGCTCTTCTCCAACATCG ATCCCATCTATGAGTTCCACAGAGGCTTCCTGCGCGAGGTGGAGCAGAGGCTGGCACTCTGGGAAGGGCC CTCCAAAGCCCACACAAAAGGCAGTCATCAACGAATCGGGGACATCCTGCTCAGGAACATGCGCCAGTTA AAGGTGTTCCAGCTCCACGAAGGGCATGTAGCAGGGGTCACAAAAATGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239195 representing NM_001282983 Red=Cloning site Green=Tags(s) MGEIEGTYRVLQTAGMRLGAQTPVGVSTLEPGQTLLPRMQEKHLHLRVKLLDNTMEIFDIEPKCDGQVLL TQVWKRLNLVECDYFGMEFQNTQSYWIWLEPMKPIIRQIRRPKNVVLRLAVKFFPPDPGQLQEEYTRYLF ALQLKRDLLEERLTCADTTAALLTSHLLQSEIGDYDETLDREHLKVNEYLPGQQHCLEKILEFHQKHVGQ TPAESDFQVLEIARKLEMYGIRFHMASDREGTKIQLAVSHMGVLVFQGTTKINTFNWSKVRKLSFKRKRF LIKLHPEVHGPYQDTLEFLLGSRDECKNFWKICVEYHTFFRLLDQPKPKAKAVFFSRGSSFRYSGRTQKQ LVDYFKDSGMKRIPYERRHSKTHTSVRALTADLPKQSISFPEGLRTPASPSSANAFYSLSPSTLVPSGLP EFKDSSSSLTDPQVSYVKSPAAERRSGAVAGGPDTPSAQPLGPPALQPGPGLSTKSPQPSPSSRKSPLSL SPAFQVPLGPAEQGSSPLLSPVLSDAGGAGMDCEEPRHKRVPADEAYFIVKEILATERTYLKDLEVITVW FRSAVVKEDAMPATLMTLLFSNIDPIYEFHRGFLREVEQRLALWEGPSKAHTKGSHQRIGDILLRNMRQL KVFQLHEGHVAGVTKME TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282983
ORF Size	1941 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282983.2
RefSeq Size	2651 bp
RefSeq ORF	1944 bp
Locus ID	9855
UniProt ID	O94887
Protein Pathways	Adherens junction
MW	73.7 kDa
Gene Summary	Functions as guanine nucleotide exchange factor that activates RAC1. May have relatively low activity. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton. Plays a role in TNFSF11-mediated osteoclast differentiation, especially in podosome rearrangement and reorganization of the actin cytoskeleton. Regulates the activation of ITGB3, integrin signaling and cell adhesion (By similarity).[UniProtKB/Swiss-Prot Function]

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