PIB5PA (INPP5J) (NM_001284289) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	INPP5; PIB5PA; PIPP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239180 representing NM_001284289 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTCCCAAGGCTTGGCACACAGAGTACAGGGCCTGGCAGGTGCCTGAGCCCCAACCTTCAGGCCC AAGAAGCCCCAGCCCCAGTCACCACCTCCTCTTCTACATCCACCCTGTCATCCTCCCCTTGGTCAGCTCA GCCTACCTGGAAGAGCGACCCCGGCTTCCGGATCACTGTGGTCACATGGAACGTGGGCACTGCCATGCCC CCAGACGATGTCACATCCCTCCTCCACCTGGGCGGTGGTGACGACAGCGACGGCGCAGACATGATCGCCA TAGGGTTGCAGGAAGTGAACTCCATGCTCAACAAGCGACTCAAGGACGCCCTCTTCACGGACCAGTGGAG TGAGCTGTTCATGGATGCGCTAGGGCCCTTCAACTTCGTGCTGGTGAGTTCGGTGAGGATGCAGGGTGTC ATCCTGCTGCTGTTCGCCAAGTACTACCACCTGCCCTTCCTGCGAGACGTGCAGACCGACTGCACGCGCA CTGGCCTGGGCGGCTACTGGGGTAACAAGGGTGGCGTGAGCGTGCGCCTGGCGGCCTTCGGGCACATGCT CTGCTTCCTGAACTGCCACTTGCCTGCGCATATGGACAAGGCGGAGCAGCGCAAAGACAACTTCCAGACC ATCCTCAGCCTCCAGCAGTTCCAAGGGCCGGGCGCACAGGGCATCCTGGATCATGACCTCGTGTTCTGGT TCGGGGACCTGAACTTCCGCATTGAGAGCTATGACCTGCACTTTGTCAAGTTTGCCATCGACAGTGACCA GCTCCATCAGCTCTGGGAGAAGGACCAGCTCAACATGGCCAAGAACACCTGGCCCATTCTGAAGGGCTTT CAGGAGGGGCCCCTCAACTTCGCTCCCACCTTCAAGTTTGATGTGGGTACCAACAAATACGATACCAGTG CCAAGAAACGGAAGCCAGCTTGGACAGACCGTATCCTATGGAAGGTCAAGGCTCCAGGTGGGGGTCCCAG CCCCTCAGGACGGAAGAGCCACCGACTCCAGGTGACGCAGCACAGCTACCGCAGCCACATGGAATACACA GTCAGCGACCACAAGCCTGTGGCTGCCCAGTTCCTCCTGCAGTTTGCCTTCAGGGACGACATGCCACTGG TGCGGCTGGAGGTGGCAGATGAGTGGGTGCGGCCCGAGCAGGCGGTGGTGAGGTACCGCATGGAAACAGT GTTCGCCCGCAGCTCCTGGGACTGGATCGGCTTATACCGGGTGGGTTTCCGCCATTGCAAGGACTATGTG GCTTATGTCTGGGCCAAACATGAAGATGTGGATGGGAATACCTACCAGGTAACATTCAGTGAGGAATCAC TGCCCAAGGGCCATGGAGACTTCATCCTGGGCTACTATAGTCACAACCACAGCATCCTCATCGGCATCAC TGAACCCTTCCAGATCTCGCTGCCTTCCTCGGAGTTGGCCAGCAGCAGCACAGACAGCTCAGGCACCAGC TCAGAGGGAGAGGATGACAGCACACTGGAGCTCCTTGCACCCAAGTCCCGCAGCCCCAGTCCTGGCAAGT CCAAGCGACACCGCAGCCGCAGCCCGGGACTGGCCAGGTTCCCTGGGCTTGCCCTACGGCCCTCATCCCG TGAACGCCGTGGTGCCAGCCGTAGCCCCTCACCCCAGAGCCGCCGCCTGTCCCGAGTGGCTCCTGACAGG AGCAGTAATGGCAGCAGCCGGGGCAGTAGTGAAGAGGGGCCCTCTGGGTTGCCTGGCCCCTGGGCCTTCC CACCAGCTGTGCCTCGAAGCCTGGGCCTGTTGCCCGCCTTGCGCCTAGAGACTGTAGACCCTGGTGGTGG TGGCTCCTGGGGACCTGATCGGGAGGCCCTGGCGCCCAACAGCCTGTCTCCTAGTCCCCAGGGCCATCGG GGGCTGGAGGAAGGGGGCCTGGGGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239180 representing NM_001284289 Red=Cloning site Green=Tags(s) MALPRLGTQSTGPGRCLSPNLQAQEAPAPVTTSSSTSTLSSSPWSAQPTWKSDPGFRITVVTWNVGTAMP PDDVTSLLHLGGGDDSDGADMIAIGLQEVNSMLNKRLKDALFTDQWSELFMDALGPFNFVLVSSVRMQGV ILLLFAKYYHLPFLRDVQTDCTRTGLGGYWGNKGGVSVRLAAFGHMLCFLNCHLPAHMDKAEQRKDNFQT ILSLQQFQGPGAQGILDHDLVFWFGDLNFRIESYDLHFVKFAIDSDQLHQLWEKDQLNMAKNTWPILKGF QEGPLNFAPTFKFDVGTNKYDTSAKKRKPAWTDRILWKVKAPGGGPSPSGRKSHRLQVTQHSYRSHMEYT VSDHKPVAAQFLLQFAFRDDMPLVRLEVADEWVRPEQAVVRYRMETVFARSSWDWIGLYRVGFRHCKDYV AYVWAKHEDVDGNTYQVTFSEESLPKGHGDFILGYYSHNHSILIGITEPFQISLPSSELASSSTDSSGTS SEGEDDSTLELLAPKSRSPSPGKSKRHRSRSPGLARFPGLALRPSSRERRGASRSPSPQSRRLSRVAPDR SSNGSSRGSSEEGPSGLPGPWAFPPAVPRSLGLLPALRLETVDPGGGGSWGPDREALAPNSLSPSPQGHR GLEEGGLGP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284289
ORF Size	1917 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284289.1, NP_001271218.1
RefSeq Size	2603 bp
RefSeq ORF	1920 bp
Locus ID	27124
UniProt ID	Q15735
Protein Families	Druggable Genome
Protein Pathways	Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
MW	70.9 kDa
Gene Summary	Inositol 5-phosphatase, which converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate. Also converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate in vitro. May be involved in modulation of the function of inositol and phosphatidylinositol polyphosphate-binding proteins that are present at membranes ruffles (By similarity).[UniProtKB/Swiss-Prot Function]

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