BRSK2 (NM_001282218) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C11orf7; PEN11B; SAD1; SADA; STK29
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239091 representing NM_001282218 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTGGAGCGGGAGATCGCGATCCTGAAGCTCATTGAGCACCCCCACGTCCTAAAGCTGCACGACG TTTATGAAAACAAAAAATATTTGTACCTGGTGCTAGAACACGTGTCAGGTGGTGAGCTCTTCGACTACCT GGTGAAGAAGGGGAGGCTGACGCCTAAGGAGGCTCGGAAGTTCTTCCGGCAGATCATCTCTGCGCTGGAC TTCTGCCACAGCCACTCCATATGCCACAGGGATCTGAAACCTGAAAACCTCCTGCTGGACGAGAAGAACA ACATCCGCATCGCAGACTTTGGCATGGCGTCCCTGCAGGTTGGCGACAGCCTGTTGGAGACCAGCTGTGG GTCCCCCCACTACGCCTGCCCCGAGGTGATCCGGGGGGAGAAGTATGACGGCCGGAAGGCGGACGTGTGG AGCTGCGGCGTCATCCTGTTCGCCTTGCTGGTGGGGGCTCTGCCCTTCGACGATGACAACTTGCGACAGC TGCTGGAGAAGGTGAAGCGGGGCGTGTTCCACATGCCGCACTTTATCCCGCCCGACTGCCAGAGTCTGCT ACGGGGCATGATCGAGGTGGACGCCGCACGCCGCCTCACGCTAGAGCACATTCAGAAACACATATGGTAT ATAGGGGGCAAGAATGAGCCCGAACCAGAGCAGCCCATTCCTCGCAAGGTGCAGATCCGCTCGCTGCCCA GCCTGGAGGACATCGACCCCGACGTGCTGGACAGCATGCACTCACTGGGCTGCTTCCGAGACCGCAACAA GCTGCTGCAGGACCTGCTGTCCGAGGAGGAGAACCAGGAGAAGATGATTTACTTCCTCCTCCTGGACCGG AAAGAAAGGTACCCGAGCCAGGAGGATGAGGACCTGCCCCCCCGGAACGAGATAGACCCTCCCCGGAAGC GTGTGGACTCCCCGATGCTGAACCGGCACGGCAAGCGGCGGCCAGAACGCAAATCCATGGAGGTGCTCAG CGTGACGGACGGCGGCTCCCCGGTGCCTGCGCGGCGGGCCATTGAGATGGCCCAGCACGGCCAGAGGTCT CGGTCCATCAGCGGTGCCTCCTCAGGCCTTTCCACCAGCCCACTCAGCAGCCCCCGGGTGACCCCTCACC CCTCACCAAGGGGCAGTCCCCTCCCCACCCCCAAGGGGACACCTGTCCACACGCCAAAGGAGAGCCCGGC TGGCACGCCCAACCCCACGCCCCCGTCCAGCCCCAGCGTCGGAGGGGTGCCCTGGAGGGCGCGGCTCAAC TCCATCAAGAACAGCTTTCTGGGCTCACCCCGCTTCCACCGCCGGAAACTGCAAGTTCCGACGCCGGAGG AGATGTCCAACCTGACACCAGAGTCGTCCCCAGAGCTGGCGAAGAAGTCCTGGTTTGGGAACTTCATCAG CCTGGAGAAGGAGGAGCAGATCTTCGTGGTCATCAAAGACAAACCTCTGAGCTCCATCAAGGCTGACATC GTGCACGCCTTCCTGTCGATTCCCAGTCTCAGCCACAGCGTCATCTCCCAAACGAGCTTCCGGGCCGAGT ACAAGGCCACGGGGGGGCCAGCCGTGTTCCAGAAGCCGGTCAAGTTCCAGGTTGATATCACCTACACGGA GGGTGGGGAGGCGCAGAAGGAGAACGGCATCTACTCCGTCACCTTCACCCTGCTCTCAGGCCCCAGCCGT CGCTTCAAGAGGGTGGTGGAGACCATCCAGGCCCAGCTGCTGAGCACACACGACCCGCCTGCGGCCCAGC ACTTGTCAGAACCCCCCCCACCAGCGCCAGGACTAAGCTGGGGTGCTGGGCTTAAGGGCCAGAAGGTGGC CACCAGCTACGAGAGTAGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239091 representing NM_001282218 Red=Cloning site Green=Tags(s) MKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIISALD FCHSHSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIRGEKYDGRKADVW SCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVDAARRLTLEHIQKHIWY IGGKNEPEPEQPIPRKVQIRSLPSLEDIDPDVLDSMHSLGCFRDRNKLLQDLLSEEENQEKMIYFLLLDR KERYPSQEDEDLPPRNEIDPPRKRVDSPMLNRHGKRRPERKSMEVLSVTDGGSPVPARRAIEMAQHGQRS RSISGASSGLSTSPLSSPRVTPHPSPRGSPLPTPKGTPVHTPKESPAGTPNPTPPSSPSVGGVPWRARLN SIKNSFLGSPRFHRRKLQVPTPEEMSNLTPESSPELAKKSWFGNFISLEKEEQIFVVIKDKPLSSIKADI VHAFLSIPSLSHSVISQTSFRAEYKATGGPAVFQKPVKFQVDITYTEGGEAQKENGIYSVTFTLLSGPSR RFKRVVETIQAQLLSTHDPPAAQHLSEPPPPAPGLSWGAGLKGQKVATSYESSL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282218
ORF Size	1842 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282218.2
RefSeq Size	4127 bp
RefSeq ORF	1845 bp
Locus ID	9024
UniProt ID	Q8IWQ3
Protein Families	Druggable Genome, Protein Kinase
MW	69.2 kDa
Gene Summary	Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-174 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-260 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress.[UniProtKB/Swiss-Prot Function]

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