AASDH (NM_001286670) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ACSF4; LYS2; NRPS998; NRPS1098
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239089 representing NM_001286670 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCTAAAGATGCTTCAGTAAAAGAATACATCTTTAAAGAACTGCAGAAATATCTTCCAAGTCATG CAGTCCCGGATGAGCTTGTATTGATCGACTCTCTACCATTTACATCCCACGGCAAAATTGATGTTTCTGA GTTAAACAAGATATATTTAAACTACATAAACTTGAAGTCTGAGAATAAGCTCAGTGGGAAAGAGGACCTT TGGGAAAAATTACAGTATTTGTGGAAGTCTACTCTGAATCTCCCAGAAGATCTTTTGAGGGTTCCTGATG AGTCACTCTTCTTAAATAGTGGTGGAGATTCCTTAAAGTCCATCCGGCTCCTCAGTGAGATTGAAAAACT TGTTGGTACATCAGTACCTGGGCTTCTGGAAATTATTCTCAGCAGTTCCATTTTAGAGATTTATAATCAC ATCCTTCAAACAGTGGTTCCAGATGAAGATGTGACATTCAGGAAGAGTTGTGCCACAAAAAGGAAACTCA GCGACATTAATCAAGAGGAAGCCAGTGGAACATCTTTACATCAGAAAGCCATCATGACTTTCACTTGCCA CAATGAGATTAATGCTTTTGTTGTACTGAGCAGAGGGAGTCAAATTTTGTCTCTGAATTCCACTAGGTTT TTAACAAAGTTAGGACATTGCTCTTCAGCCTGTCCTTCTGACTCAGTTTCACAGACCAACATTCAAAATT TGAAAGGCTTAAATTCTCCAGTTCTTATTGGGAAGTCAAAAGATCCATCCTGTGTTGCAAAAGTTTCTGA AGAGGGGAAACCTGCGATAGGGACTCAGAAAATGGAGTTACATGTGAGGTGGAGGTCAGACACAGGCAAA TGTGTAGATGCTTCACCGCTGGTTGTAATACCCACTTTTGATAAGTCATCTACAACTGTGTACATTGGTT CCCATTCTCATAGAATGAAGGCAGTTGACTTTTACTCTGGGAAGGTAAAATGGGAACAGATTTTGGGAGA TCGAATTGAATCCTCAGCATGTGTATCTAAGTGTGGAAACTTTATTGTGGTGGGCTGTTATAATGGATTA GTTTATGTTCTGAAAAGTAATAGTGGAGAAAAATACTGGATGTTTACTACTGAAGATGCTGTCAAAAGCT CGGCAACCATGGATCCAACCACAGGACTCATTTACATTGGATCTCATGACCAGCACGCATATGCTTTAGA TATTTATAGAAAGAAGTGTGTTTGGAAGTCAAAATGTGGAGGAACTGTCTTTTCCTCTCCGTGTTTGAAC CTGATTCCACATCATTTGTATTTTGCTACATTGGGAGGACTTTTACTGGCTGTAAATCCTGCTACTGGGA ACGTTATTTGGAAACATTCCTGTGGAAAACCACTCTTCTCTTCCCCACAATGTTGCTCACAGTATATTTG TATTGGCTGTGTAGATGGGAATTTACTCTGCTTTACTCACTTTGGAGAACAGGTTTGGCAGTTCTCTACC AGTGGACCAATCTTTTCATCCCCGTGTACCTCACCATCAGAGCAAAAAATATTTTTTGGTTCCCATGATT GCTTTATCTACTGTTGTAACATGAAAGGTCACCTGCAGTGGAAATTTGAAACTACTTCAAGGGTCTATGC AACACCGTTTGCTTTCCATAACTACAATGGCAGCAATGAAATGTTGCTGGCAGCAGCATCTACTGATGGG AAAGTGTGGATCTTGGAATCTCAGAGTGGACAATTGCAAAGTGTTTATGAACTTCCTGGAGAAGTCTTCT CTTCTCCTGTGGTCCTGGAATCAATGCTCATTATTGGGTGTAGAGATAATTATGTTTATTGTCTGGATTT ATTGGGTGGCAATCAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239089 representing NM_001286670 Red=Cloning site Green=Tags(s) MVSKDASVKEYIFKELQKYLPSHAVPDELVLIDSLPFTSHGKIDVSELNKIYLNYINLKSENKLSGKEDL WEKLQYLWKSTLNLPEDLLRVPDESLFLNSGGDSLKSIRLLSEIEKLVGTSVPGLLEIILSSSILEIYNH ILQTVVPDEDVTFRKSCATKRKLSDINQEEASGTSLHQKAIMTFTCHNEINAFVVLSRGSQILSLNSTRF LTKLGHCSSACPSDSVSQTNIQNLKGLNSPVLIGKSKDPSCVAKVSEEGKPAIGTQKMELHVRWRSDTGK CVDASPLVVIPTFDKSSTTVYIGSHSHRMKAVDFYSGKVKWEQILGDRIESSACVSKCGNFIVVGCYNGL VYVLKSNSGEKYWMFTTEDAVKSSATMDPTTGLIYIGSHDQHAYALDIYRKKCVWKSKCGGTVFSSPCLN LIPHHLYFATLGGLLLAVNPATGNVIWKHSCGKPLFSSPQCCSQYICIGCVDGNLLCFTHFGEQVWQFST SGPIFSSPCTSPSEQKIFFGSHDCFIYCCNMKGHLQWKFETTSRVYATPFAFHNYNGSNEMLLAAASTDG KVWILESQSGQLQSVYELPGEVFSSPVVLESMLIIGCRDNYVYCLDLLGGNQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286670
ORF Size	1839 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286670.2
RefSeq Size	3017 bp
RefSeq ORF	1842 bp
Locus ID	132949
UniProt ID	Q4L235
Protein Pathways	Lysine biosynthesis, Lysine degradation, Metabolic pathways
MW	68.3 kDa
Gene Summary	This gene encodes a member of the non-ribosome peptide syntesase (NRPS) enzyme family. The encoded protein contains an AMP-binding domain, PP-binding (phosphopantetheine, or pantetheine 4'phosphate-binding) domain and the Pyrrolo-quinoline quinon (PQQ) binding domain. The protein is expressed in several adult tissues. [provided by RefSeq, Apr 2016]

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