RPS6KC1 (NM_001287220) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	humS6PKh1; RPK118; RSKL1; S6K-delta-1; S6PKh1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239049 representing NM_001287220 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTAAAGCTCTGCCTTTGAAGAGTAGTCTTACTCCAAGTTCTCAAGATGACAGCAACCAGGAAGATG ATGGCCAAGATAGCTCTCCAAAGTGGCCAGATTCTGGTTCAAGTTCAGAAGAAGAATGTACTACTAGTTA TTTAACATTATGCAATGAATATGGGCAAGAAAAGATTGAACCAGGGTCTTTGAATGAGGAGCCCTTCATG AAGACTGAAGGGAATGGTGTTGATACAAAAGCTATTAAAAGCTTCCCAGCACACCTTGCTGCTGACAGTG ACAGCCCCAGCACACAGCTGAGAGCTCACGAGCTGAAGTTCTTCCCCAACGATGACCCAGAAGCAGTTAG TTCTCCAAGAACATCAGATTCCCTCAGTAGATCAAAAAATAGCCCCATGGAATTCTTTAGGATAGACAGT AAGGATAGCGCAAGTGAACTCCTGGGACTTGACTTTGGAGAAAAATTGTATAGTCTAAAATCAGAACCTT TGAAACCATTCTTTACTCTTCCAGATGGAGACAGTGCTTCTAGGAGTTTTAATACTAGTGAAAGCAAGGT AGAGTTTAAAGCTCAGGACACCATTAGCAGGGGCTCAGATGACTCAGTGCCAGTTATTTCATTTAAAGAT GCTGCTTTTGATGATGTCAGTGGTACTGATGAAGGAAGACCTGATCTTCTTGTAAATTTACCTGGTGAAT TGGAGTCAACAAGAGAAGCTGCAGCAATGGGACCTACTAAGTTTACACAAACTAATATAGGGATAATAGA AAATAAACTCTTGGAAGCCCCTGATGTTTTATGCCTCAGGCTTAGTACTGAACAATGCCAAGCACATGAG GAGAAAGGCATAGAGGAACTGAGTGATCCCTCTGGGCCCAAATCCTATAGTATAACAGAGAAACACTATG CACAGGAGGATCCCAGGATGTTATTTGTAGCAGCTGTTGATCATAGTAGTTCAGGAGATATGTCTTTGTT ACCCAGCTCAGATCCTAAGTTTCAAGGACTTGGAGTGGTTGAGTCAGCAGTAACTGCAAACAACACAGAA GAAAGCTTATTCCGTATTTGTAGTCCACTCTCAGGTGCTAATGAATATATTGCAAGCACAGACACTTTAA AAACAGAAGAAGTATTGCTGTTTACAGATCAGACTGATGATTTGGCTAAAGAGGAACCAACTTCTTTATT CCAGAGAGACTCTGAGACTAAGGGTGAAAGTGGTTTAGTGCTAGAAGGAGACAAGGAAATACATCAGATT TTTGAGGACCTTGATAAAAAATTAGCACTAGCCTCCAGGTTTTACATCCCAGAGGGCTGCATTCAAAGAT GGGCAGCTGAAATGGTGGTAGCCCTTGATGCTTTACATAGAGAGGGAATTGTGTGCCGCGATTTGAACCC AAACAACATCTTATTGAATGATAGAGGACACATTCAGCTAACGTATTTTAGCAGGTGGAGTGAGGTTGAA GATTCCTGTGACAGCGATGCCATAGAGAGAATGTACTGTGCCCCAGAGGTTGGAGCAATCACTGAAGAAA CTGAAGCCTGTGATTGGTGGAGTTTGGGTGCTGTCCTCTTTGAACTTCTCACTGGCAAGACTCTGGTTGA ATGCCATCCAGCAGGAATAAATACTCACACTACTTTGAACATGCCAGAATGTGTCTCTGAAGAGGCTCGC TCACTCATTCAACAGCTCTTGCAGTTCAATCCTCTGGAACGACTTGGTGCTGGAGTTGCTGGTGTTGAAG ATATCAAATCTCATCCATTTTTTACCCCTGTGGATTGGGCAGAACTGATGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239049 representing NM_001287220 Red=Cloning site Green=Tags(s) MLKALPLKSSLTPSSQDDSNQEDDGQDSSPKWPDSGSSSEEECTTSYLTLCNEYGQEKIEPGSLNEEPFM KTEGNGVDTKAIKSFPAHLAADSDSPSTQLRAHELKFFPNDDPEAVSSPRTSDSLSRSKNSPMEFFRIDS KDSASELLGLDFGEKLYSLKSEPLKPFFTLPDGDSASRSFNTSESKVEFKAQDTISRGSDDSVPVISFKD AAFDDVSGTDEGRPDLLVNLPGELESTREAAAMGPTKFTQTNIGIIENKLLEAPDVLCLRLSTEQCQAHE EKGIEELSDPSGPKSYSITEKHYAQEDPRMLFVAAVDHSSSGDMSLLPSSDPKFQGLGVVESAVTANNTE ESLFRICSPLSGANEYIASTDTLKTEEVLLFTDQTDDLAKEEPTSLFQRDSETKGESGLVLEGDKEIHQI FEDLDKKLALASRFYIPEGCIQRWAAEMVVALDALHREGIVCRDLNPNNILLNDRGHIQLTYFSRWSEVE DSCDSDAIERMYCAPEVGAITEETEACDWWSLGAVLFELLTGKTLVECHPAGINTHTTLNMPECVSEEAR SLIQQLLQFNPLERLGAGVAGVEDIKSHPFFTPVDWAELMR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001287220
ORF Size	1803 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287220.2
RefSeq Size	4038 bp
RefSeq ORF	1806 bp
Locus ID	26750
Protein Families	Druggable Genome, Protein Kinase
MW	66.6 kDa
Gene Summary	Sphingosine kinase catalyzes the formation of sphingosine 1 phosphate, a lipid cellular messenger. The protein encoded by this gene can bind to sphingosine kinase and to phosphatidylinositol 3-phosphate, suggesting a role in sphingosine 1 phophate signaling. The encoded protein can also bind to peroxiredoxin-3 and may help transport it to mitochondria. [provided by RefSeq, Mar 2017]

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