FMO1 (NM_001282692) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238784 representing NM_001282692 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGAGAACATGGCCAAGCGAGTTGCCATTGTGGGAGCTGGGGTCAGCGGCCTGGCCTCCATCAAGT GCTGTCTGGAAGAAGGACTGGAGCCCACCTGCTTTGAGAGGAGCGATGACCTTGGGGGGCTGTGGAGATT CACCGAACATGTTGAAGAAGGCAGAGCCAGTCTCTACAAGTCTGTGGTTTCCAACAGCTGCAAGGAGATG TCTTGTTACTCAGACTTTCCATTCCCAGAAGATTATCCAAACTATGTGCCAAATTCTCAATTCCTGGAAT ATCTCAAAATGTATGCAAACCACTTTGACCTTCTGAAACACATTCAATTCAAGACCAAAGTCTGCAGTGT AACAAAATGCTCAGATTCTGCTGTCTCTGGCCAATGGGAGGTGGTCACTATGCATGAAGAGAAGCAAGAG TCAGCCATCTTTGATGCTGTCATGGTCTGCACTGGCTTTCTTACTAATCCTTATTTGCCACTGGATTCCT TTCCAGGTATTAATGCCTTTAAAGGCCAGTACTTTCATAGCCGGCAATATAAGCATCCAGATATATTTAA GGACAAGAGAGTCCTTGTGATTGGAATGGGAAATTCTGGCACAGACATTGCTGTGGAGGCCAGCCACCTG GCGGAAAAGGTGTTCCTCAGCACCACCGGAGGGGGATGGGTGATCAGCCGAATCTTTGACTCGGGCTACC CATGGGACATGGTGTTCATGACACGCTTTCAGAACATGTTGAGAAATTCCCTCCCAACCCCAATTGTGAC TTGGTTGATGGAGCGAAAGATAAACAACTGGCTCAATCATGCAAATTACGGCTTAATACCAGAAGACAGG ACTCAGCTGAAAGAGTTTGTGCTAAATGATGAGCTCCCAGGACGCATCATCACTGGGAAAGTGTTCATCA GGCCAAGCATAAAAGAGGTAAAGGAAAACTCTGTCATATTTAACAATACTTCAAAGGAAGAGCCTATTGA CATCATTGTCTTTGCCACTGGATACACATTTGCTTTCCCCTTCCTTGATGAGTCTGTAGTGAAAGTTGAA GATGGCCAGGCCTCACTGTACAAGTATATCTTCCCTGCACATCTGCAAAAGCCAACCCTGGCCATTATTG GCCTCATCAAACCCTTGGGCTCCATGATACCTACAGGAGAAACACAAGCTCGGTGGGCTGTTCGAGTCCT GAAAGGTGTAAATAAGTTACCACCACCAAGTGTCATGATAGAGGAAATTAATGCAAGGAAAGAAAACAAG CCCAGTTGGTTTGGCTTGTGCTACTGCAAGGCTTTACAATCAGATTATATCACATACATAGATGAACTCC TGACCTATATCAATGCAAAACCCAACCTGTTCTCTATGCTCCTAACGGATCCACATCTGGCTCTGACCGT CTTCTTTGGCCCATGCTCACCATACCAGTTCCGCTTGACTGGCCCAGGAAAATGGGAAGGAGCCAGAAAT GCCATCATGACCCAGTGGGACCGAACATTCAAGGTCATCAAAGCTCGAGTTGTACAAGAGTCTCCATCTC CCTTTGAAAGTTTTCTTAAAGTCTTTAGCTTTCTGGCTTTGCTTGTGGCTATTTTTCTGATTTTCCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238784 representing NM_001282692 Red=Cloning site Green=Tags(s) MQENMAKRVAIVGAGVSGLASIKCCLEEGLEPTCFERSDDLGGLWRFTEHVEEGRASLYKSVVSNSCKEM SCYSDFPFPEDYPNYVPNSQFLEYLKMYANHFDLLKHIQFKTKVCSVTKCSDSAVSGQWEVVTMHEEKQE SAIFDAVMVCTGFLTNPYLPLDSFPGINAFKGQYFHSRQYKHPDIFKDKRVLVIGMGNSGTDIAVEASHL AEKVFLSTTGGGWVISRIFDSGYPWDMVFMTRFQNMLRNSLPTPIVTWLMERKINNWLNHANYGLIPEDR TQLKEFVLNDELPGRIITGKVFIRPSIKEVKENSVIFNNTSKEEPIDIIVFATGYTFAFPFLDESVVKVE DGQASLYKYIFPAHLQKPTLAIIGLIKPLGSMIPTGETQARWAVRVLKGVNKLPPPSVMIEEINARKENK PSWFGLCYCKALQSDYITYIDELLTYINAKPNLFSMLLTDPHLALTVFFGPCSPYQFRLTGPGKWEGARN AIMTQWDRTFKVIKARVVQESPSPFESFLKVFSFLALLVAIFLIFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282692
ORF Size	1608 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282692.1, NP_001269621.1
RefSeq Size	2198 bp
RefSeq ORF	1611 bp
Locus ID	2326
UniProt ID	Q01740
Protein Families	Druggable Genome
Protein Pathways	Drug metabolism - cytochrome P450
MW	61.3 kDa
Gene Summary	Metabolic N-oxidation of the diet-derived amino-trimethylamine (TMA) is mediated by flavin-containing monooxygenase and is subject to an inherited FMO3 polymorphism in man resulting in a small subpopulation with reduced TMA N-oxidation capacity resulting in fish odor syndrome Trimethylaminuria. Three forms of the enzyme, FMO1 found in fetal liver, FMO2 found in adult liver, and FMO3 are encoded by genes clustered in the 1q23-q25 region. Flavin-containing monooxygenases are NADPH-dependent flavoenzymes that catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

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