MYH (MUTYH) (NM_001293190) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MYH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238781 representing NM_001293190 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACCGCTCGTCTCCCGCCTGAGTCGTCTGTGGGCCATCATGAGGAAGCCACGAGCAGCCGTGGGAA GTGGTCACAGGAAGCAGGCAGCCAGCCAGGAAGGGAGGCAGAAGCATGCTAAGAACAACAGTCAGGCCAA GCCTTCTGCCTGTGATGCAGGCCTGGCCAGGCAGCCGGAAGAGGTGGTATTGCAGGCCTCTGTCTCCTCA TACCATCTATTCAGAGACGTAGCTGAAGTCACAGCCTTCCGAGGGAGCCTGCTAAGCTGGTACGACCAAG AGAAACGGGACCTACCATGGAGAAGACGGGCAGAAGATGAGATGGACCTGGACAGGCGGGCATATGCTGT GTGGGTCTCAGAGGTCATGCTGCAGCAGACCCAGGTTGCCACTGTGATCAACTACTATACCGGATGGATG CAGAAGTGGCCTACACTGCAGGACCTGGCCAGTGCTTCCCTGGAGGAGGTGAATCAACTCTGGGCTGGCC TGGGCTACTATTCTCGTGGCCGGCGGCTGCAGGAGGGAGCTCGGAAGGTGGTAGAGGAGCTAGGGGGCCA CATGCCACGTACAGCAGAGACCCTGCAGCAGCTCCTGCCTGGCGTGGGGCGCTACACAGCTGGGGCCATT GCCTCTATCGCCTTTGGCCAGGCAACCGGTGTGGTGGATGGCAACGTAGCACGGGTGCTGTGCCGTGTCC GAGCCATTGGTGCTGATCCCAGCAGCACCCTTGTTTCCCAGCAGCTCTGGGGTCTAGCCCAGCAGCTGGT GGACCCAGCCCGGCCAGGAGATTTCAACCAAGCAGCCATGGAGCTAGGGGCCACAGTGTGTACCCCACAG CGCCCACTGTGCAGCCAGTGCCCTGTGGAGAGCCTGTGCCGGGCACGCCAGAGAGTGGAGCAGGAACAGC TCTTAGCCTCAGGGAGCCTGTCGGGCAGTCCTGACGTGGAGGAGTGTGCTCCCAACACTGGACAGTGCCA CCTGTGCCTGCCTCCCTCGGAGCCCTGGGACCAGACCCTGGGAGTGGTCAACTTCCCCAGAAAGGCCAGC CGCAAGCCCCCCAGGGAGGAGAGCTCTGCCACCTGTGTTCTGGAACAGCCTGGGGCCCTTGGGGCCCAAA TTCTGCTGGTGCAGAGGCCCAACTCAGGTCTGCTGGCAGGACTGTGGGAGTTCCCGTCCGTGACCTGGGA GCCCTCAGAGCAGCTTCAGCGCAAGGCCCTGCTGCAGGAACTACAGCGTTGGGCTGGGCCCCTCCCAGCC ACGCACCTCCGGCACCTTGGGGAGGTTGTCCACACCTTCTCTCACATCAAGCTGACATATCAAGTATATG GGCTGGCCTTGGAAGGGCAGACCCCAGTGACCACCGTACCACCAGGTGCTCGCTGGCTGACGCAGGAGGA ATTTCACACCGCAGCTGTTTCCACCGCCATGAAAAAGGTTTTCCGTGTGTATCAGGGCCAACAGCCAGGG ACCTGTATGGGTTCCAAAAGGTCCCAGGTGTCCTCTCCGTGCAGTCGGAAAAAGCCCCGCATGGGCCAGC AAGTCCTGGATAATTTCTTTCGGTCTCACATCTCCACTGATGCACACAGCCTCAACAGTGCAGCCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238781 representing NM_001293190 Red=Cloning site Green=Tags(s) MTPLVSRLSRLWAIMRKPRAAVGSGHRKQAASQEGRQKHAKNNSQAKPSACDAGLARQPEEVVLQASVSS YHLFRDVAEVTAFRGSLLSWYDQEKRDLPWRRRAEDEMDLDRRAYAVWVSEVMLQQTQVATVINYYTGWM QKWPTLQDLASASLEEVNQLWAGLGYYSRGRRLQEGARKVVEELGGHMPRTAETLQQLLPGVGRYTAGAI ASIAFGQATGVVDGNVARVLCRVRAIGADPSSTLVSQQLWGLAQQLVDPARPGDFNQAAMELGATVCTPQ RPLCSQCPVESLCRARQRVEQEQLLASGSLSGSPDVEECAPNTGQCHLCLPPSEPWDQTLGVVNFPRKAS RKPPREESSATCVLEQPGALGAQILLVQRPNSGLLAGLWEFPSVTWEPSEQLQRKALLQELQRWAGPLPA THLRHLGEVVHTFSHIKLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPG TCMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293190
ORF Size	1608 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293190.1, NP_001280119.1
RefSeq Size	1906 bp
RefSeq ORF	1611 bp
Locus ID	4595
UniProt ID	Q9UIF7
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Base excision repair
MW	59.6 kDa
Gene Summary	This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. This gene product is thought to play a role in signaling apoptosis by the introduction of single-strand breaks following oxidative damage. Mutations in this gene result in heritable predisposition to colorectal cancer, termed MUTYH-associated polyposis (MAP). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2017]

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