CATSPER2 (NM_001282310) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238768 representing NM_001282310 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCTGGGCTCAGACATGGCCGCTTACCAACAAGAAGAGCAGATGCAGCTTCCCCGAGCTGATGCCA TTCGTTCACGTCTCATCGATACTTTCTCTCTCATTGAGCATTTGCAAGGCTTGAGCCAAGCTGTGCCGCG GCACACTATCAGGGAGTTACTTGATCCTTCCCGCCAGAAGAAACTTGTATTGGGAGATCAACACCAGCTA GTGCGTTTCTCTATAAAGCCTCAGCGTATAGAACAGATTTCACATGCCCAGAGGCTGTTGAGCAGGCTTC ATGTGCGCTGCAGTCAGAGGCCACCTCTTTCTTTGTGGGCCGGATGGGTCCTTGAGTGTCCTCTCTTCAA AAACTTCATCATCTTCCTGGTCTTTTTGAATACGATCATATTGATGGTTGAAATAGAATTGCTGGAATCC ACAAATACCAAACTATGGCCATTGAAGCTGACCTTGGAGGTGGCAGCTTGGTTTATCTTGCTTATTTTCA TCCTGGAGATCCTTCTTAAGTGGCTATCCAACTTTTCTGTTTTCTGGAAGAGTGCCTGGAATGTCTTTGA CTTTGTTGTTACCATGTTGTCCCTGCTTCCCGAGGTTGTGGTATTGGTAGGGGTAACAGGCCAATCGGTG TGGCTTCAGCTTCTGAGGATCTGCCGGGTGCTGAGGTCTCTCAAACTCCTTGCACAATTCCGTCAAATTC AAATTATTATTTTGGTCCTGGTCAGGGCCCTCAAGAGCATGACCTTCCTCTTGATGTTGCTGCTCATCTT CTTCTACATTTTTGCTGTGACTGGTGTCTACGTCTTCTCAGAGTACACCCGTTCACCTCGTCAGGACCTG GAGTACCATGTGTTCTTCTCGGACCTCCCGAATTCCCTGGTAACAGTGTTCATTCTCTTCACCTTGGATC ATTGGTATGCACTGCTTCAGGACGTCTGGAAGGTGCCTGAAGTCAGTCGCATCTTCAGCAGCATCTATTT CATCCTTTGGTTGTTGCTTGGCTCCATTATCTTTCGAAGTATCATAGTAGCCATGATGGTTACTAACTTT CAGAATATCAGGAAAGAGCTGAATGAGGAGATGGCGCGTCGGGAGGTTCAGCTCAAAGCTGACATGTTCA AGCGGCAGATCATCCAGAGGAGAAAAAACATGTCACATGAAGCACTGACGTCAAGCCATAGCAAAATAGA GGACAGAGGAGCTAGTCAACAAAGGGAAAGTTTGGACTTATCAGAAGTGTCTGAAGTAGAGTCTAATTAT GGTGCCACTGAAGAGGATTTAATAACATCTGCATCAAAAACAGAAGAGACCTTGTCAAAAAAGAGAGAGT ACCAGTCTTCCTCCTGTGTCTCCTCCACATCCTCTTCCTATTCTTCCTCTTCTGAATCCAGATTTTCTGA ATCTATTGGTCGTTTGGACTGGGAGACTCTTGTGCACGAAAATCTGCCCGGGCTAATGGAAATGGATCAG GATGACCGTGTTTGGCCCAGAGACTCACTCTTCCGATATTTTGAGTTGCTAGAAAAGCTTCAGTATAACC TAGAGGAACGTAAGAAGTTACAAGAGTTTGCAGTGCAGGCACTGATGAACTTGGAAGACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238768 representing NM_001282310 Red=Cloning site Green=Tags(s) MVLGSDMAAYQQEEQMQLPRADAIRSRLIDTFSLIEHLQGLSQAVPRHTIRELLDPSRQKKLVLGDQHQL VRFSIKPQRIEQISHAQRLLSRLHVRCSQRPPLSLWAGWVLECPLFKNFIIFLVFLNTIILMVEIELLES TNTKLWPLKLTLEVAAWFILLIFILEILLKWLSNFSVFWKSAWNVFDFVVTMLSLLPEVVVLVGVTGQSV WLQLLRICRVLRSLKLLAQFRQIQIIILVLVRALKSMTFLLMLLLIFFYIFAVTGVYVFSEYTRSPRQDL EYHVFFSDLPNSLVTVFILFTLDHWYALLQDVWKVPEVSRIFSSIYFILWLLLGSIIFRSIIVAMMVTNF QNIRKELNEEMARREVQLKADMFKRQIIQRRKNMSHEALTSSHSKIEDRGASQQRESLDLSEVSEVESNY GATEEDLITSASKTEETLSKKREYQSSSCVSSTSSSYSSSSESRFSESIGRLDWETLVHENLPGLMEMDQ DDRVWPRDSLFRYFELLEKLQYNLEERKKLQEFAVQALMNLEDK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282310
ORF Size	1602 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282310.2
RefSeq Size	1755 bp
RefSeq ORF	1605 bp
Locus ID	117155
Protein Families	Druggable Genome, Ion Channels: Other, Transmembrane
MW	62.9 kDa
Gene Summary	This gene encodes a member of a family of cation channel proteins that localize to the flagellum of spermatozoa. Defects at this locus causes male infertility. Alternatively spliced transcript variants have been observed at this locus. Readthrough transcription originates upstream of this locus in diphosphoinositol pentakisphosphate kinase 1 pseudogene 1 and is represented by GeneID:110006325. Related pseudogenes are found next to this locus on chromosome 15 and on chromosome 5. [provided by RefSeq, Mar 2017]

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