ADA2 (NM_001282226) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ADGF; CECR1; IDGFL; PAN; SNEDS; VAIHS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238646 representing NM_001282226 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGTGGATGGCCCATCTGAGCGGCCAGCCCTGTGCTTCTTGCTGTTGGCTGTGGCAATGTCTTTCT TCGGCTCAGCTCTATCCATAGATGAAACACGGGCGCATCTGTTGTTGAAAGAAAAGATGATGCGGCTGGG GGGGCGGCTGGTGCTGAACACCAAGGAGGAGCTGGCCAATGAGAGGCTCATGACGCTCAAAATCGCTGAG ATGAAGGAGGCCATGAGGACCCTGATATTCCCACCCAGCATGCACTTTTTCCAGGCCAAGCATCTCATTG AGAGAAGTCAAGTGTTTAATATTCTAAGGATGATGCCAAAAGGGGCTGCCTTGCACCTCCATGACATTGG CATCGTGACTATGGACTGGCTGGTGAGGAATGTCACCTACAGGCCTCACTGCCACATCTGTTTCACCCCA AGGGGGATCATGCAGTTCAGATTTGCTCACCCAACTCCCCGTCCATCAGAAAAATGTTCCAAGTGGATTC TGCTGGAGGATTATCGGAAGCGGGTGCAGAACGTCACTGAGTTTGATGACAGCTTGCTGAGGAATTTCAC TCTGGTGACCCAGCACCCGGAGGTGATTTACACAAACCAAAATGTTGTCTGGTCGAAATTTGAAACCATC TTCTTCACCATCTCTGGTCTCATCCATTACGCACCAGTGTTCAGAGACTATGTCTTCCGGAGCATGCAGG AGTTCTACGAGGACAACGTGCTCTACATGGAGATCAGAGCCAGGCTGCTGCCGGTGTATGAGCTCAGTGG AGAGCACCATGACGAAGAGTGGTCAGTGAAGACTTACCAGGAAGTAGCTCAGAAGTTTGTGGAAACTCAC CCTGAGTTTATTGGAATCAAAATCATTTATTCGGATCACAGATCCAAAGATGTGGCTGTCATCGCAGAAT CCATCCGAATGGCCATGGGGCTCCGAATCAAGTTCCCCACGGTGGTGGCAGGGTTTGACCTGGTGGGGCA TGAGGACACTGGCCACTCCTTGCATGACTACAAGGAAGCTCTGATGATCCCCGCCAAGGATGGCGTTAAG CTGCCTTACTTCTTCCACGCCGGAGAAACAGACTGGCAGGGTACTTCCATAGACAGGAACATTCTGGATG CTCTGATGCTGAACACTACCAGAATCGGCCATGGATTTGCTTTGAGCAAACACCCCGCAGTCAGGACTTA CTCCTGGAAAAAGGACATCCCCATAGAAGTCTGTCCCATCTCTAACCAGGTGCTGAAACTGGTGTCTGAC TTGAGGAACCACCCTGTAGCCACTCTGATGGCCACTGGGCACCCCATGGTGATCAGCTCTGATGACCCAG CTATGTTTGGTGCCAAAGGCTTGTCCTATGATTTCTATGAGGTCTTCATGGGCATTGGGGGGATGAAGGC TGACCTGAGGACCCTCAAACAGCTGGCCATGAACTCTATCAAGTACAGTACCCTGTTGGAGAGTGAGAAA AATACTTTCATGGAAATCTGGAAGAAGAGATGGGATAAGTTCATAGCAGATGTGGCTACAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238646 representing NM_001282226 Red=Cloning site Green=Tags(s) MLVDGPSERPALCFLLLAVAMSFFGSALSIDETRAHLLLKEKMMRLGGRLVLNTKEELANERLMTLKIAE MKEAMRTLIFPPSMHFFQAKHLIERSQVFNILRMMPKGAALHLHDIGIVTMDWLVRNVTYRPHCHICFTP RGIMQFRFAHPTPRPSEKCSKWILLEDYRKRVQNVTEFDDSLLRNFTLVTQHPEVIYTNQNVVWSKFETI FFTISGLIHYAPVFRDYVFRSMQEFYEDNVLYMEIRARLLPVYELSGEHHDEEWSVKTYQEVAQKFVETH PEFIGIKIIYSDHRSKDVAVIAESIRMAMGLRIKFPTVVAGFDLVGHEDTGHSLHDYKEALMIPAKDGVK LPYFFHAGETDWQGTSIDRNILDALMLNTTRIGHGFALSKHPAVRTYSWKKDIPIEVCPISNQVLKLVSD LRNHPVATLMATGHPMVISSDDPAMFGAKGLSYDFYEVFMGIGGMKADLRTLKQLAMNSIKYSTLLESEK NTFMEIWKKRWDKFIADVATK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282226
ORF Size	1533 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282226.2
RefSeq Size	4477 bp
RefSeq ORF	1536 bp
Locus ID	51816
UniProt ID	Q9NZK5
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
MW	59.4 kDa
Gene Summary	This gene encodes a member of a subfamily of the adenosine deaminase protein family. The encoded protein is one of two adenosine deaminases found in humans, which regulate levels of the signaling molecule, adenosine. The encoded protein is secreted from monocytes undergoing differentiation and may regulate cell proliferation and differentiation. This gene may be responsible for some of the phenotypic features associated with cat eye syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

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