PXK (NM_001289099) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MONAKA; SLOB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238561 representing NM_001289099 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGTGAATTCATAGCTGAAAGGCAGAAAGGTCTTCAGAACTATCTCAACGTGATCACAACAAATC ATATCTTGTCTAATTGTGAGCTGGTTAAGAAGTTTTTAGATCCAAACAACTATTCCGCAAACTATACTGA GATTGCCTTGCAACAGGTTTCCATGTTCTTCCGATCAGAACCAAAGTGGGAGGTGGTGGAACCTTTGAAA GACATAGGTTGGAGAATAAGGAAGAAATATTTCTTGATGAAGATTAAAAATCAGCCAAAGGAACGGCTAG TGTTAAGCTGGGCTGACCTTGGCCCAGACAAGTATTTGTCAGATAAAGATTTTCAGTGTCTAATCAAACT TCTGCCTTCTTGTTTGCACCCTTACATCTATCGGGTTACCTTTGCCACAGCTAATGAATCCTCAGCGTTG CTAATTAGGATGTTTAACGAAAAGGGAACATTGAAGGATCTGATCTACAAGGCAAAACCAAAAGACCCAT TTCTAAAGAAGTACTGCAACCCTAAGAAGATTCAGGGCCTGGAACTCCAGCAAATAAAAACATATGGACG GCAAATATTAGAGGTACTGAAGTTTCTTCATGACAAGGGATTCCCTTATGGGCATCTTCACGCCTCCAAT GTGATGCTCGATGGGGACACTTGCCGGCTGCTGGACCTTGAGAATTCCTTATTGGGCCTGCCTTCCTTCT ACCGATCTTATTTTTCACAATTCAGGAAAATCAATACATTGGAAAGTGTGGATGTCCACTGCTTTGGCCA CTTACTGTATGAAATGACTTATGGACGACCGCCAGACTCGGTGCCTGTGGACTCCTTCCCTCCTGCCCCG TCCATGGCTGTGGTGGCCGTGTTGGAGTCTACGCTGTCTTGTGAAGCCTGTAAAAATGGCATGCCTACCA TCTCCCGGCTCTTACAGATGCCATTATTCAGCGATGTTTTACTAACCACTTCTGAAAAACCACAGTTTAA GATCCCTACAAAGTTAAAAGAGGCATTGAGAATTGCCAAAGAATGTATAGAGAAGAGACTAATTGAGGAA CAGAAACAGATTCACCAGCATCGAAGACTGACAAGAGCTCAGTCCCACCATGGATCTGAGGAGGAAAGAA AAAAAAGAAAGATTTTAGCTCGAAAGAAGTCAAAACGATCTGCTCTTGAAAATAGTGAAGAGCATTCAGC GAAGTACAGCAACTCCAATAATTCAGCAGGATCTGGGGCCAGCTCACCTCTCACGTCCCCGTCATCGCCA ACTCCACCCTCTACATCAGGGATATCTGCATTACCTCCACCTCCTCCACCTCCACCACCACCAGCAGCTC CCTTGCCTCCTGCGAGCACCGAGGCACCTGCCCAGCTCTCGTCTCAGGCTGTGAATGGCATGAGCCGAGG GGCCTTGCTCAGCTCCATCCAGAATTTCCAAAAAGGAACTTTGAGGAAAGCCAAAACCTGTGATCACAGT GCTCCGAAGATCGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238561 representing NM_001289099 Red=Cloning site Green=Tags(s) MDREFIAERQKGLQNYLNVITTNHILSNCELVKKFLDPNNYSANYTEIALQQVSMFFRSEPKWEVVEPLK DIGWRIRKKYFLMKIKNQPKERLVLSWADLGPDKYLSDKDFQCLIKLLPSCLHPYIYRVTFATANESSAL LIRMFNEKGTLKDLIYKAKPKDPFLKKYCNPKKIQGLELQQIKTYGRQILEVLKFLHDKGFPYGHLHASN VMLDGDTCRLLDLENSLLGLPSFYRSYFSQFRKINTLESVDVHCFGHLLYEMTYGRPPDSVPVDSFPPAP SMAVVAVLESTLSCEACKNGMPTISRLLQMPLFSDVLLTTSEKPQFKIPTKLKEALRIAKECIEKRLIEE QKQIHQHRRLTRAQSHHGSEEERKKRKILARKKSKRSALENSEEHSAKYSNSNNSAGSGASSPLTSPSSP TPPSTSGISALPPPPPPPPPPAAPLPPASTEAPAQLSSQAVNGMSRGALLSSIQNFQKGTLRKAKTCDHS APKIG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289099
ORF Size	1485 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289099.1, NP_001276028.1
RefSeq Size	3125 bp
RefSeq ORF	1488 bp
Locus ID	54899
UniProt ID	Q7Z7A4
Protein Families	Druggable Genome, Protein Kinase
MW	56.2 kDa
Gene Summary	This gene encodes a phox (PX) domain-containing protein which may be involved in synaptic transmission and the ligand-induced internalization and degradation of epidermal growth factors. Variations in this gene may be associated with susceptibility to systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

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