GCNF (NR6A1) (NM_001278546) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CT150; GCNF; GCNF1; hGCNF; hRTR; NR61; RTR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238448 representing NM_001278546 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGGACGAACCGCCGCCTAGCGGAGGGGGAGGCGGCGGGGGCTCGGCGGGGTTCCTGGAGCCTC CCGCCGCGCTCCCTCCGCCGCCGCGCAACGGTTTCTGTCAGGATGAATTGGCAGAGCTTGACCCAGGCAC TAATGATCGGGCTGAACAACGAACCTGTCTCATTTGTGGGGACCGCGCTACAGGCTTGCACTATGGGATC ATCTCCTGTGAGGGCTGCAAAGGGTTTTTCAAGCGGAGCATTTGCAACAAACGGGTATATCGATGCAGTC GTGACAAGAACTGTGTCATGTCTCGGAAGCAGAGGAACAGGTGCCAGTACTGCCGCCTGCTCAAATGCCT CCAGATGGGGATGAACCGGAAGGCTATCAGAGAAGATGGCATGCCTGGAGGCCGGAATAAGAGCATTGGG CCAGTCCAGATATCGGAAGAAGAAATCGAAAGGATCATGTCTGGGCAGGAGTTTGAGGAAGAGGCCAATC ACTGGAGCAACCATGGTGATAGTGACCACAGTTCCCCTGGGAACAGGGCTTCGGAGAGCAACCAGCCCTC ACCAGGCTCCACACTGTCTTCCAGTAGGTCTGTGGAACTGAATGGATTCATGGCCTTCAGGGAACAGTAC ATGGGAATGTCTGTGCCTCCACATTACCAATATATACCGCACCTTTTTAGCTATTCTGGCCACTCACCAC TTCTGCCCCAACAAGCTCGCAGCCTGGATCCCCAGTCATACAGTCTGATTCACCAGCTGTTATCAGCCGA GGACCTGGAACCATTGGGCACGCCCATGTTGATTGAAGATGGATACGCTGTGACACAGGCAGAACTATTT GCCCTGCTTTGCCGCCTGGCCGACGAGCTGCTCTTTAGGCAGATTGCCTGGATCAAGAAACTGCCTTTCT TCTGCGAGCTCTCAATCAAGGATTACACGTGCCTCTTGAGCTCTACGTGGCAGGAGCTAATCCTGCTGTC TTCCCTCACCGTTTACAGCAAGCAGATCTTTGGGGAACTGGCTGATGTCACTGCCAAGTACTCGCCCTCC GATGAAGAACTACACAGATTTAGTGATGAAGGGATGGAGGTGATCGAGCGGCTCATCTACCTCTATCACA AGTTCCATCAGCTAAAGGTCAGCAACGAGGAGTATGCTTGCATGAAAGCAATTAACTTCCTAAATCAAGA TATCAGGGGTCTGACCAGTGCCTCACAGCTGGAACAATTGAATAAACGATACTGGTACATTTGCCAGGAT TTTACTGAATATAAATACACACATCAGCCGAACCGCTTTCCTGATCTCATGATGTGCTTACCTGAGATTC GATATATTGCAGGAAAGATGGTGAATGTGCCCCTGGAGCAGCTGCCCCTCCTCTTTAAGGTGGTGCTGCA TTCCTGCAAGACCAGTGTGGGCAAGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238448 representing NM_001278546 Red=Cloning site Green=Tags(s) MERDEPPPSGGGGGGGSAGFLEPPAALPPPPRNGFCQDELAELDPGTNDRAEQRTCLICGDRATGLHYGI ISCEGCKGFFKRSICNKRVYRCSRDKNCVMSRKQRNRCQYCRLLKCLQMGMNRKAIREDGMPGGRNKSIG PVQISEEEIERIMSGQEFEEEANHWSNHGDSDHSSPGNRASESNQPSPGSTLSSSRSVELNGFMAFREQY MGMSVPPHYQYIPHLFSYSGHSPLLPQQARSLDPQSYSLIHQLLSAEDLEPLGTPMLIEDGYAVTQAELF ALLCRLADELLFRQIAWIKKLPFFCELSIKDYTCLLSSTWQELILLSSLTVYSKQIFGELADVTAKYSPS DEELHRFSDEGMEVIERLIYLYHKFHQLKVSNEEYACMKAINFLNQDIRGLTSASQLEQLNKRYWYICQD FTEYKYTHQPNRFPDLMMCLPEIRYIAGKMVNVPLEQLPLLFKVVLHSCKTSVGKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278546
ORF Size	1428 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278546.2
RefSeq Size	7052 bp
RefSeq ORF	1431 bp
Locus ID	2649
UniProt ID	Q15406
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
MW	54.4 kDa
Gene Summary	This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2013]

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