TTC23 (NM_001288615) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HCC-8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238286 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGAATCACAGGAAACCCACATATCCAACCACCTAGATGAAGTTGTTGCTGCTGTTAGCATCACTC ATAGAAAGAAGTTCCAAAACAAGCTGCTTCAGACAGCACTATTCCAGCCTCCTCGAGAGAAACTCCACCT CTGTGAAGAGAAAGCAAAGTCCTATTCCAACAGTCATGAGTACAAACAGGCCGTCCATGAGCTTGTGCGT TGCGTAGCACTGACAAGAATTTGCTATGGAGACTCACATTGGAAACTAGCAGAGGCACATGTTAATCTGG CTCAAGGCTACCTCCAGCTGAAAGGACTGTCACTGCAAGCAAAACAACATGCAGAAAAAGCCAGACAAAT CCTCGCCAACTCCATTGTGCCTCCCTATAGTGAGAATACAGATGTTTTCAAGTTTTCCATTGAGCTTTTC CATACCATGGGCAGAGCTTTACTCTCCCTTCAAAAATTTAAGGAAGCTGCAGAGAATTTGACAAAAGCAG AGAGACTTTCAAAGGAGCTGCTACAATGTGGAAGAATTATAAAGGAAGAATGGATAGAAATTGAAGCACG GATCAGATTATCATTTGCACAGGTGTATCAAGGTCAGAAGAAGTCAAAAGAAGCTTTGTCCCACTATCAA GCAGCTTTGGAATATGTTGAGATCAGTAAAGGTGAAACAAGTCGTGAGTGTGTACCCATATTGAGAGAAT TAGCAGGTGTAGAGCAAGCCCTGGGACTCCACGATGTATCCATCAACCACTTCCTCCAGGCACATCTCAT CATCCTGAGTAGAAGCCCCTCTCAAGTGGAGGCAGCAGACTCGGCACACATCGTCGCCCATGCTGCTGTC GCTTCAGGGAGACACGAGCACCATGATGTAGCTGAGCAGTATTTTCAAGAGAGCATGGCTCATCTTAAGG ATTCTGAAGGGATGGGAAGAACCAAATTTCTTTCAATTCAAGATGAATTTTGCCATTTTCTACAAATGAC TGGACAAAAAGAGAGAGCAACCTCGATCCTGAGAGAGTCCCTGGAAGCCAAAGTGGAAGCATTTGGCGAT TTCAGTCCCGAGGTGGCAGAGACATACCGGCTCCTGGGAGGAGCAGACCTGGCGCAGGGGAACCACAGTG GGGCCCGCAAGAAACTGAAGAAGTGTCTCCAGATCCAGACCCTCTTATATGGACCGCAGGACAAAAGGAC TCTGGCCACCCAGCAGGCCATGGGCATGCTGTCCACGGCCCCCAAGGTTGCTTCGAAGCCAAGGCAGGCA TCAAAAGCCAAAGTGGCCTTCTGCACCAGCATCCCTCAGGACACCCTGCTGGGGAAGGCCCGGCCCGGCA CAACAGCAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238286 protein sequence Red=Cloning site Green=Tags(s) MQESQETHISNHLDEVVAAVSITHRKKFQNKLLQTALFQPPREKLHLCEEKAKSYSNSHEYKQAVHELVR CVALTRICYGDSHWKLAEAHVNLAQGYLQLKGLSLQAKQHAEKARQILANSIVPPYSENTDVFKFSIELF HTMGRALLSLQKFKEAAENLTKAERLSKELLQCGRIIKEEWIEIEARIRLSFAQVYQGQKKSKEALSHYQ AALEYVEISKGETSRECVPILRELAGVEQALGLHDVSINHFLQAHLIILSRSPSQVEAADSAHIVAHAAV ASGRHEHHDVAEQYFQESMAHLKDSEGMGRTKFLSIQDEFCHFLQMTGQKERATSILRESLEAKVEAFGD FSPEVAETYRLLGGADLAQGNHSGARKKLKKCLQIQTLLYGPQDKRTLATQQAMGMLSTAPKVASKPRQA SKAKVAFCTSIPQDTLLGKARPGTTAD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001288615
ORF Size	1341 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288615.1, NP_001275544.1
RefSeq Size	3933 bp
RefSeq ORF	1344 bp
Locus ID	64927
UniProt ID	Q5W5X9
MW	50 kDa
Gene Summary	Participates positively in the ciliary Hedgehog (Hh) signaling.[UniProtKB/Swiss-Prot Function]

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