ABI2 (NM_001282927) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ABI-2; ABI2B; AblBP3; AIP-1; AIP1; argBP1; argBPIA; argBPIB; SSH3BP2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237991 representing NM_001282927 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCTGCAGATGCTGGATATCCAGGCATCCCAGCTACGAAGGATGGAATCTTCAATCAATCATATTTC ACAAGCAAATTAGAGGCGTTGATCTTGAGTCGACTTTTGTGACCAAATTTGGAAACAATTGCAGTTTGAG ATTGAATGAGACAGTTGATATTCATAAAGAGAAAGTTGCAAGAAGAGAAATTGGTATTTTGACTACCAAT AAAAACACTTCAAGGACACATAAGATTATTGCTCCAGCCAACCTTGAACGACCAGTTCGTTATATTAGAA AACCTATTGACTATACAATTCTAGATGATATTGGACATGGAGTAAAGGTGAGTACCCAGAACATGAAGAT GGGTGGGCTGCCGCGTACAACACCTCCAACTCAGAAGCCCCCTAGTCCCCCTATGTCAGGGAAAGGGACA CTTGGGCGGCACTCCCCCTATCGCACACTGGAGCCAGTGCGTCCTCCAGTGGTACCAAATGATTACGTAC CTAGCCCAACCCGTAATATGGCTCCCTCGCAGCAGAGCCCTGTGAGGACAGCTTCTGTGAATCAAAGAAA TCGAACTTACAGCAGCAGTGGGAGTAGTGGAGGGAGCCACCCAAGTAGTCGGAGCAGCAGTCGAGAGAAC AGTGGAAGTGGTAGTGTGGGGGTTCCTATTGCTGTTCCTACTCCATCTCCTCCCAGTGTCTTTCCAGGTC ATCCTGTACAGTTCTACAGCATGAATAGGCCTGCCTCTCGCCATACTCCCCCAACAATAGGGGGCTCGTT GCCCTATAGACGCCCTCCTTCCATTACTTCACAAACAAGCCTTCAGAATCAGATGAATGGAGGACCTTTT TATAGCCAGAATCCAGTTTCAGATACACCACCTCCACCGCCACCTGTGGAAGAACCAGTCTTTGATGAGT CTCCCCCACCTCCTCCTCCTCCAGAAGATTACGAAGAGGAGGAAGCTGCTGTGGTTGAGTATAGTGATCC TTATGCTGAAGAGGACCCACCGTGGGCTCCACGTTCTTACTTGGAAAAGGTTGTGGCAATTTATGACTAT ACAAAAGACAAGGAAGATGAGCTGTCCTTTCAGGAAGGAGCCATTATTTATGTCATCAAGAAGAATGACG ATGGTTGGTATGAGGGAGTTATGAATGGAGTGACTGGGCTTTTTCCTGGGAATTACGTTGAGTCTATCAT GCATTATTCTGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237991 representing NM_001282927 Red=Cloning site Green=Tags(s) MSCRCWISRHPSYEGWNLQSIIFHKQIRGVDLESTFVTKFGNNCSLRLNETVDIHKEKVARREIGILTTN KNTSRTHKIIAPANLERPVRYIRKPIDYTILDDIGHGVKVSTQNMKMGGLPRTTPPTQKPPSPPMSGKGT LGRHSPYRTLEPVRPPVVPNDYVPSPTRNMAPSQQSPVRTASVNQRNRTYSSSGSSGGSHPSSRSSSREN SGSGSVGVPIAVPTPSPPSVFPGHPVQFYSMNRPASRHTPPTIGGSLPYRRPPSITSQTSLQNQMNGGPF YSQNPVSDTPPPPPPVEEPVFDESPPPPPPPEDYEEEEAAVVEYSDPYAEEDPPWAPRSYLEKVVAIYDY TKDKEDELSFQEGAIIYVIKKNDDGWYEGVMNGVTGLFPGNYVESIMHYSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282927
ORF Size	1203 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282927.2
RefSeq Size	6122 bp
RefSeq ORF	1206 bp
Locus ID	10152
UniProt ID	Q9NYB9
Protein Pathways	Regulation of actin cytoskeleton
MW	44.9 kDa
Gene Summary	Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation (PubMed:21107423). Acts as regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge (PubMed:7590236, PubMed:8649853, PubMed:10498863). Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (By similarity). In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (By similarity). Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (By similarity). Also required for nascent adherens junction assembly in epithelial cells (PubMed:15572692).[UniProtKB/Swiss-Prot Function]

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