ULK3 (NM_001284365) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237685 representing NM_001284365 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCAATTAGCTAGCGCCCTGCAATTCCTGCATGAACGGAATATCTCTCACCTGGATCTGAAGCCAC AGAACATTCTACTGAGCTCCTTGGAGAAGCCCCACCTAAAACTGGCAGACTTTGGTTTCGCACAACACAT GTCCCCGTGGGATGAGAAGCACGTGCTCCGTGGCTCCCCCCTCTACATGGCCCCCGAGATGGTGTGCCAG CGGCAGTATGACGCCCGCGTGGACCTCTGGTCCATGGGGGTCATCCTGTATGAAGCCCTCTTCGGGCAGC CCCCCTTTGCCTCCAGGTCGTTCTCGGAGCTGGAAGAGAAGATCCGTAGCAACCGGGTCATCGAGCTCCC CTTGCGGCCCCTGCTCTCCCGAGACTGCCGGGACCTACTGCAGCGGCTCCTGGAGCGGGACCCCAGCCGT CGCATCTCCTTCCAGGACTTTTTTGCGCACCCCTGGGTGGACCTGGAGCACATGCCCAGTGGGGAGAGTC TGGGGCGAGCAACCGCCCTGGTGGTGCAGGCTGTGAAGAAAGACCAGGAGGGGGATTCAGCAGCTGCCTT ATCACTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCCCTGCACTATGAAGTGGATGCCCAGCGGAAG GAGGCAATTAAGGCAAAGGTGGGGCAGTACGTGTCCCGGGCTGAGGAGCTCAAGGCCATCGTCTCCTCTT CCAATCAGGCCCTGCTGAGGCAGGGGACCTCTGCCCGAGACCTGCTCAGAGAGATGGCCCGGGACAAGCC ACGCCTCCTAGCTGCCCTGGAAGTGGCTTCAGCTGCCATGGCCAAGGAGGAGGCCGCCGGCGGGGAGCAG GATGCCCTGGACCTGTACCAGCACAGCCTGGGGGAGCTACTGCTGTTGCTGGCAGCGGAGCCCCCGGGCC GGAGGCGGGAGCTGCTTCACACTGAGGTTCAGAACCTCATGGCCCGAGCTGAATACTTGAAGGAGCAGGT CAAGATGAGGGAATCTCGCTGGGAAGCTGACACCCTGGACAAAGAGGGACTGTCGGAATCTGTTCGTAGC TCTTGCACCCTTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237685 representing NM_001284365 Red=Cloning site Green=Tags(s) MQQLASALQFLHERNISHLDLKPQNILLSSLEKPHLKLADFGFAQHMSPWDEKHVLRGSPLYMAPEMVCQ RQYDARVDLWSMGVILYEALFGQPPFASRSFSELEEKIRSNRVIELPLRPLLSRDCRDLLQRLLERDPSR RISFQDFFAHPWVDLEHMPSGESLGRATALVVQAVKKDQEGDSAAALSLYCKALDFFVPALHYEVDAQRK EAIKAKVGQYVSRAEELKAIVSSSNQALLRQGTSARDLLREMARDKPRLLAALEVASAAMAKEEAAGGEQ DALDLYQHSLGELLLLLAAEPPGRRRELLHTEVQNLMARAEYLKEQVKMRESRWEADTLDKEGLSESVRS SCTLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284365
ORF Size	1065 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284365.3
RefSeq Size	2567 bp
RefSeq ORF	1068 bp
Locus ID	25989
UniProt ID	Q6PHR2
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	mTOR signaling pathway, Regulation of autophagy
MW	40.7 kDa
Gene Summary	Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.[UniProtKB/Swiss-Prot Function]

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