EI24 (NM_001290135) Human Tagged ORF Clone

CAT#: RC237455

  • TrueORF®

EI24 (myc-DDK-tagged) - Human etoposide induced 2.4 (EI24), transcript variant 3

ORF Plasmid: DDK tGFP



  "NM_001290135" in other vectors (2)

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CNY 3,990.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-EI24 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms EPG4; PIG8; TP53I8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC237455 representing NM_001290135
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGACAGTGTCAAAACCTTTCTCCAGGACCTTGCCAGACTAGATGCTCGAATCCAGCAAAAGAGAG
AGGAGCAGCGTCGAAGAAGGGCAAGTAGTGTCTTGGCACAGAGAAGAGCCCAGAGTATAGAGCGGAAGCA
AGAGAGTGAGCCACGTATTGTTAGTAGAATTTTCCAGTGTTGTGCTTGGAATGGTGGAGTGTTCTGGTTC
AGTCTCCTCTTGTTTTATCGAGTATTTATTCCTGTGCTTCAGTCGGTAACAGCCCGAATTATCGGTGACC
CATCACTACATGGAGATGTTTGGTCGTGGCTGGAATTCTTCCTCACGTCAATTTTCAGTGCTCTTTGGGT
GCTCCCCTTGTTTGTGCTTAGCAAAGTGGTGAATGCCATTTGGTTTCAGGATATAGCTGACCTGGCATTT
GAGGTATCAGGGAGGAAGCCTCACCCATTCCCTAGTGTCAGCAAAATAATTGCTGACATGCTCTTCAACC
TTTTGCTGCAGGCTCTTTTCCTCATTCAGGGAATGTTTGTGAGTCTCTTTCCCATCCATCTTGTCGGTCA
GCTGGTTAGTCTCCTGCATATGTCCCTTCTCTACTCACTGTACTGCTTTGAATATCGTTGGTTCAATAAA
GGAATTGAAATGCACCAGCGGTTGTCTAACATAGAAAGGAATTGGCCTTACTACTTTGGGTTTGGTTTGC
CCTTGGCTTTTCTCACAGCAATGCAGTCCTCATATATTATCAGTGGCTGCCTTTTCTCTATCCTCTTTCC
TTTATTCATTATCAGCGCCAATGAAGCAAAGACCCCTGGCAAAGCATATCTCTTCCAGTTGCGCCTCTTC
TCCTTGGTGGTCTTCTTAAGCAACAGACTCTTCCACAAGACAGTCTACCTGCAGTCGGCCCTGAGCAGCT
CTACTTCTGCAGAGAAGTTCCCTTCACCGCATCCGTCGCCTGCCAAACTGAAGGCTACTGCAGGTCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC237455 representing NM_001290135
Red=Cloning site Green=Tags(s)

MADSVKTFLQDLARLDARIQQKREEQRRRRASSVLAQRRAQSIERKQESEPRIVSRIFQCCAWNGGVFWF
SLLLFYRVFIPVLQSVTARIIGDPSLHGDVWSWLEFFLTSIFSALWVLPLFVLSKVVNAIWFQDIADLAF
EVSGRKPHPFPSVSKIIADMLFNLLLQALFLIQGMFVSLFPIHLVGQLVSLLHMSLLYSLYCFEYRWFNK
GIEMHQRLSNIERNWPYYFGFGLPLAFLTAMQSSYIISGCLFSILFPLFIISANEAKTPGKAYLFQLRLF
SLVVFLSNRLFHKTVYLQSALSSSTSAEKFPSPHPSPAKLKATAGH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001290135
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001290135.2
RefSeq Size 2244 bp
RefSeq ORF 981 bp
Locus ID 9538
UniProt ID O14681
Protein Families Druggable Genome, Transmembrane
Protein Pathways p53 signaling pathway
MW 37.9 kDa
Gene Summary This gene encodes a putative tumor suppressor and has higher expression in p53-expressing cells than in control cells and is an immediate-early induction target of p53-mediated apoptosis. The encoded protein may suppress cell growth by inducing apoptotic cell death through the caspase 9 and mitochondrial pathways. This gene is located on human chromosome 11q24, a region frequently altered in cancers. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, 7, and 8. [provided by RefSeq, Feb 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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