GTF2A1 (NM_001278940) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	TF2A1; TFIIA; TFIIA-42; TFIIAL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237454 representing NM_001278940 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTCCAGGGCAGTAGATGGATTTCATTCAGAAGAGCAGCAGCTTCTACTGCAAGTTCAACAGCAGC ATCAACCCCAGCAGCAGCAGCATCACCACCATCACCATCATCAGCAAGCTCAGCCTCAGCAGACAGTACC TCAGCAAGCGCAGACCCAGCAGGTTCTTATTCCTGCATCACAGCAAGCCACAGCACCACAAGTTATTGTT CCAGATTCTAAGTTGATACAGCATATGAATGCATCAAACATGAGTGCTGCTGCTACAGCTGCTACCTTAG CACTCCCTGCAGGTGTGACTCCTGTTCAGCAGATATTAACAAATTCAGGCCAGCTTCTTCAGGTGGTCAG AGCAGCCAATGGTGCCCAATATATCTTTCAGCCTCAGCAGTCAGTGGTTCTACAACAACAGGTTATACCA CAAATGCAGCCTGGTGGAGTACAAGCTCCTGTTATACAGCAGGTGCTGGCTCCTCTTCCTGGAGGGATTT CACCACAGACAGGTGTCATCATCCAGCCTCAGCAAATCTTATTTACAGGAAATAAGACTCAAGTTATACC TACGACAGTGGCAGCACCTACACCAGCCCAAGCACAGATAACTGCAACTGGCCAGCAGCAACCGCAGGCC CAGCCTGCTCAAACACAAGCTCCATTGGTCTTACAAGTTGATGGAACTGGGGATACATCATCTGAAGAAG ATGAAGATGAAGAAGAAGACTATGATGATGATGAGGAGGAAGACAAAGAGAAAGATGGAGCTGAAGATGG GCAGGTGGAAGAAGAGCCCCTCAATAGTGAAGATGATGTGAGTGATGAGGAAGGACAGGAACTCTTTGAC ACAGAAAATGTTGTTGTATGCCAATATGATAAGATACACAGAAGTAAAAACAAATGGAAATTTCATCTCA AGGATGGCATTATGAATCTTAATGGAAGAGATTATATATTTTCCAAAGCCATTGGAGATGCAGAATGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237454 representing NM_001278940 Red=Cloning site Green=Tags(s) MQSRAVDGFHSEEQQLLLQVQQQHQPQQQQHHHHHHHQQAQPQQTVPQQAQTQQVLIPASQQATAPQVIV PDSKLIQHMNASNMSAAATAATLALPAGVTPVQQILTNSGQLLQVVRAANGAQYIFQPQQSVVLQQQVIP QMQPGGVQAPVIQQVLAPLPGGISPQTGVIIQPQQILFTGNKTQVIPTTVAAPTPAQAQITATGQQQPQA QPAQTQAPLVLQVDGTGDTSSEEDEDEEEDYDDDEEEDKEKDGAEDGQVEEEPLNSEDDVSDEEGQELFD TENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIGDAEW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278940
ORF Size	978 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278940.2
RefSeq Size	6403 bp
RefSeq ORF	981 bp
Locus ID	2957
UniProt ID	P52655
Protein Families	Transcription Factors
Protein Pathways	Basal transcription factors
MW	36.2 kDa
Gene Summary	Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]

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