PRUNE (PRUNE1) (NM_001303229) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DRES-17; DRES17; H-PRUNE; HTCD37; NMIHBA; PRUNE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237063 representing NM_001303229 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTTAAAATTGGAAAGGCAACCCCAAAGGACAGCAAATATGTGGAGAAACTAGAGGCCCTTTTCC CAGACCTACCCAAGAGAAATGATATATTTGATTCCCTACAAAAGGCAAAGTTTGATGTATCAGGACTGAC CACTGAGCAGATGCTGAGAAAAGACCAGAAGACTATCTATAGACAAGGCGTCAAGGTGGCCATTAGTGCA ATATATATGGATTTGGAGGCCTTTCTGCAGAGGTCTAACCTCCTTGCAGATCTCCATGCTTTCTGCCAGG CTCACAGCTATGATGTCCTGGTTGCCATGACTATCTTTTTCAACACTCACAATGAGCCAGTGCGGCAGTT GGCTATTTTCTGTCCCCATGTGGCACTCCAAACAACGATCTGTGAAGTCCTGGAACGCTCCCACTCTCCA CCCCTGAAGCTGACCCCTGCCTCAAGTACCCACCCTAACCTCCATGCCTATCTTCAAGGCAACACCCAGG TCTCTCGAAAGAAACTTCTGCCCCTGCTCCAGGAAGCCCTGTCAGCATATTTTGACTCCATGAAGATCCC TTCAGGACAGCCTGAGACAGCAGATGTGTCCAGGGAGCAAGTGGACAAGGAATTGGACAGGGCAAGTAAC TCCCTGATTTCTGGCCTGAGTCAAGATGAGGAGGACCCTCCGCTGCCCCCGACGCCCATGAACAGCTTGG TGGATGAGTGCCCTCTAGATCAGGGGCTGCCTAAACTCTCTGCTGAGGCCGTCTTCGAGAAGTGCAGTCA GATCTCACTGTCACAGTCTACCACAGCCTCCCTGTCCAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237063 representing NM_001303229 Red=Cloning site Green=Tags(s) MDLKIGKATPKDSKYVEKLEALFPDLPKRNDIFDSLQKAKFDVSGLTTEQMLRKDQKTIYRQGVKVAISA IYMDLEAFLQRSNLLADLHAFCQAHSYDVLVAMTIFFNTHNEPVRQLAIFCPHVALQTTICEVLERSHSP PLKLTPASSTHPNLHAYLQGNTQVSRKKLLPLLQEALSAYFDSMKIPSGQPETADVSREQVDKELDRASN SLISGLSQDEEDPPLPPTPMNSLVDECPLDQGLPKLSAEAVFEKCSQISLSQSTTASLSKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001303229
ORF Size	813 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303229.2
RefSeq Size	2898 bp
RefSeq ORF	816 bp
Locus ID	58497
UniProt ID	Q86TP1
Protein Pathways	Purine metabolism
MW	30.6 kDa
Gene Summary	This gene encodes a member of the DHH protein superfamily of phosphoesterases. This protein has been found to function as both a nucleotide phosphodiesterase and an exopolyphosphatase. This protein is believed to stimulate cancer progression and metastases through the induction of cell motility. A pseuodgene has been identified on chromosome 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

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