Glutathione S Transferase theta 1 (GSTT1) (NM_001293807) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC236718 representing NM_001293807 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCTGGAGCTGTACCTGGACCTGCTGTCCCAGCCCTGCCGCGCTGTTTACATCTTTGCCAAGAAGA ACGACATTCCCTTCGAGCTGCGCATCGTGGATCTGATTAAAGACGGAGTCTCGCTCTGTCGCCCAGGCTG GAGTGCAGTGGCAGGATCACAGCTCACTGCAACCTCTGCCGCCCGAAACCTTGTCCTCTGCCTCTTGTTC CTGCTGGGGAGGTCAGCACTTAAGCGATGCCTTTGCCCAGGTGAACCCCCTCAAGAAGGTGCCAGCCTTG AAGGACGGGGACTTCACCTTGACGGAGAGGTGATGTTCCCTGTTTTCCTGGGTGAGCCAGTATCTCCCCA GACACTGGCAGCCACCCTGGCAGAGTTGGATGTGACCCTGCAGTTGCTCGAGGACAAGTTCCTCCAGAAC AAGGCCTTCCTTACTGGTCCTCACATCTCCTTAGCTGACCTCGTAGCCATCACGGAGCTGATGCATCCCG TGGGTGCTGGCTGCCAAGTCTTCGAAGGCCGACCCAAGCTGGCCACATGGCGGCAGCGCGTGGAGGCAGC AGTGGGGGAGGACCTCTTCCAGGAGGCCCATGAGGTCATTCTGAAGGCCAAGGACTTCCCACCTGCAGAC CCCACCATAAAGCAGAAGCTGATGCCCTGGGTGCTGGCCATGATCCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC236718 representing NM_001293807 Red=Cloning site Green=Tags(s) MGLELYLDLLSQPCRAVYIFAKKNDIPFELRIVDLIKDGVSLCRPGWSAVAGSQLTATSAARNLVLCLLF LLGRSALKRCLCPGEPPQEGASLEGRGLHLDGEVMFPVFLGEPVSPQTLAATLAELDVTLQLLEDKFLQN KAFLTGPHISLADLVAITELMHPVGAGCQVFEGRPKLATWRQRVEAAVGEDLFQEAHEVILKAKDFPPAD PTIKQKLMPWVLAMIR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293807
ORF Size	678 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293807.1, NP_001280736.1
RefSeq Size	1067 bp
RefSeq ORF	681 bp
Locus ID	2952
UniProt ID	P30711
Protein Pathways	Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450
MW	25.2 kDa
Gene Summary	The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2015]

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