CACNA1F (NM_001256790) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AIED; Cav1.4; Cav1.4alpha1; COD3; COD4; CORDX; CORDX3; CSNB2; CSNB2A; CSNBX2; JM8; JMC8; OA2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235349 representing NM_001256790 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGAATCTGAAGGCGGGAAAGGTGAGAGAATCCTTCCATCCCTGCAGACCCTTGGAGCAAGCATCG TGGAGTGGAAGCCCTTCGACATCCTCATCCTGCTGACCATCTTTGCCAACTGCGTGGCCCTGGGAGTTTA CATCCCCTTCCCTGAGGACGACTCCAACACTGCCAACCACAACCTGGAGCAGGTGGAGTACGTATTCCTG GTGATTTTCACTGTGGAGACGGTGCTCAAGATCGTGGCCTACGGGCTGGTGCTCCACCCCAGCGCCTACA TCCGCAATGGCTGGAACCTACTCGACTTCATCATCGTCGTGGTCGGGCTGTTCAGCGTTCTGCTGGAGCA GGGCCCCGGACGGCCAGGCGACGCCCCGCACACCGGGGGAAAGCCAGGAGGCTTCGATGTGAAGGCATTG AGGGCGTTTCGGGTGCTGCGGCCACTGAGGCTGGTGTCTGGGGTCCCGAGCCTGCACATAGTGCTCAATT CCATCATGAAGGCTCTGGTGCCGCTGCTGCACATTGCACTGCTCGTGCTCTTCGTCATCATCATTTATGC CATCATTGGGCTCGAGCTGTTCCTTGGACGAATGCACAAGACGTGCTACTTCCTGGGATCCGACATGGAA GCGGAGGAGGACCCATCGCCCTGTGCGTCTTCGGGATCAGGGCGTGCGTGCACGCTGAACCAGACTGAGT GCCGCGGGCGCTGGCCAGGGCCCAATGGAGGCATCACCAACTTTGACAACTTCTTCTTCGCCATGCTGAC AGTCTTCCAGTGTGTCACCATGGAAGGCTGGACCGATGTGCTCTACTGGATGCAAGATGCCATGGGGTAT GAACTGCCCTGGGTGTACTTTGTGAGCCTTGTCATCTTTGGGTCCTTCTTCGTCCTCAACCTTGTGCTTG GCGTCCTGAGTGGGGAGTTCTCCAAGGAGAGAGAGAAAGCGAAAGCTCGCGGGGACTTCCAGAAGCAGCG GGAGAAGCAGCAGATGGAGGAAGACCTGCGGGGCTACCTGGACTGGATCACTCAAGCCGAAGAGCTGGAC ATGGAGGACCCCTCCGCCGATGACAACCTTGGTTCTATGGCTGAAGAGGGCCGGGCGGGCCATCGGCCAC AGCTGGCCGAGCTGACCAATAGGAGGCGTGGACGTCTGCGCTGGTTCAGTCATTCTACTCGCTCCACACA CTCCACCAGCAGCCATGCCAGCCTCCCAGCCAGTGACACCGGTTCCATGACAGAGACCCAAGGCGATGAG GATGAGGAGGAGGGGGCTCTGGCCAGCTGTACACGCTGCCTAAACAAGATCATGAAAACCAGAGTCTGCC GCCGCCTCCGCCGAGCCAACCGGGTCCTTCGGGCACGCTGCCGTCGGGCAGTGAAGTCCAATGCCTGCTA CTGGGCTGTGCTGTTGCTCGTCTTCCTCAACACGTTGACCATCGCCTCTGAGCACCACGGGCAGCCTGTG TGGCTCACCCAGATCCAGGAGTATGCCAACAAAGTGTTGCTCTGTCTGTTCACGGTGGAGATGCTTCTCA AATTGTACGGTCTGGGCCCCTCTGCCTATGTGTCTTCCTTCTTCAACCGCTTTGACTGCTTTGTGGTCTG TGGGGGCATCCTAGAGACCACCTTGGTGGAGGTGGGTGCCATGCAGCCCTTGGGCATCTCAGTGCTCCGA TGTGTGCGCCTCCTCAGGATCTTTAAGGTCACCAGACACTGGGCTTCTCTGAGCAATCTGGTGGCATCCC TGCTCAATTCAATGAAATCCATCGCATCCTTGCTGCTTCTCCTCTTCCTCTTCATCATTATCTTCTCCCT GCTTGGCATGCAGCTGTTTGGGGGCAAGTTCAACTTTGACCAGACCCACACCAAGCGAAGCACCTTTGAC ACGTTCCCCCAGGCCCTCCTCACTGTCTTTCAGATCCTGACAGGTGAGGACTGGAACGTGGTCATGTATG ATGGTATCATGGCATATGGTGGCCCCTTCTTCCCAGGAATGTTGGTGTGCATCTATTTCATCATTCTCTT CATCTGTGGCAACTACATCCTGTTGAACGTGTTTCTTGCCATTGCTGTGGACAACCTGGCCAGTGGAGAT GCAGGCACTGCCAAGGACAAGGGCGGGGAGAAGAGCAATGAGAAGGATCTCCCACAGGAGAATGAAGGCC TGGTGCCTGGTGTGGAGAAAGAGGAAGAGGAGGGTGCAAGGAGGGAAGGAGCAGACATGGAGGAGGAGGA GGAGGAGGAAGAAGAGGAAGAAGAGGAAGAAGAGGAAGAGGGTGCAGGGGGTGTGGAACTCCTGCAGGAA GTTGTACCCAAGGAGAAGGTGGTACCCATCCCTGAGGGCAGCGCCTTCTTCTGCCTCAGCCAAACCAACC CGCTGAGGAAGGGCTGCCACACCCTCATCCACCATCATGTCTTCACCAATCTTATCCTGGTGTTCATCAT CCTCAGCAGTGTGTCCCTGGCCGCTGAGGACCCCATCCGAGCCCACTCCTTCCGCAACCATATTCTGGGT TACTTCGATTATGCCTTCACCTCCATTTTCACTGTGGAGATTCTACTAAAGATGACAGTGTTTGGGGCCT TCCTGCACCGCGGCTCCTTCTGCCGTAGCTGGTTTAATATGTTGGATCTGCTGGTGGTCAGTGTGTCCCT CATCTCCTTTGGCATCCACTCCAGCGCCATCTCGGTGGTGAAGATTCTGCGAGTACTCCGAGTACTGCGG CCCCTCCGAGCCATCAACAGGGCCAAGGGACTCAAGCATGTGGTGCAGTGTGTATTTGTGGCCATCCGGA CCATCGGAAACATCATGATTGTCACCACACTTCTGCAATTTATGTTCGCCTGCATCGGGGTGCAGCTCTT CAAGGGGAAATTCTACACCTGCACGGACGAGGCCAAACACACCCCTCAAGAATGCAAGGGCTCCTTCCTG GTATACCCAGATGGAGACGTGTCACGGCCCCTGGTCCGGGAGCGGCTCTGGGTCAACAGTGATTTCAACT TTGACAATGTCCTTTCAGCCATGATGGCCCTGTTCACTGTCTCCACCTTTGAAGGCTGGCCTGCACTGCT ATACAAGGCCATCGATGCATATGCAGAGGACCACGGCCCCATCTATAATTACCGTGTGGAGATCTCAGTG TTCTTCATTGTCTACATCATCATCATTGCGTTCTTCATGATGAACATCTTCGTGGGCTTCGTCATCATCA CTTTCCGTGCCCAGGGCGAGCAGGAGTACCAAAACTGTGAGCTGGACAAGAACCAGCGTCAATGTGTGGA ATATGCCCTCAAGGCCCAGCCACTCCGCCGTTACATCCCCAAGAACCCGCATCAGTATCGTGTGTGGGCC ACTGTGAACTCTGCTGCCTTTGAGTACCTGATGTTCCTGCTCATCCTGCTCAACACAGTTGCCCTAGCCA TGCAGCACTATGAGCAGACTGCTCCCTTCAACTATGCCATGGACATCCTCAACATGGTCTTCACTGGCCT CTTCACTATTGAGATGGTGCTCAAAATCATCGCCTTCAAGCCCAAGCATTACTTCACTGATGCCTGGAAC ACGTTTGACGCTCTTATTGTGGTGGGCAGCATAGTGGATATTGCCGTCACTGAAGTCAATAATGGTGGCC ACCTTGGCGAGAGCTCTGAGGACAGCTCCCGCATTTCCATTACCTTCTTTCGCCTCTTCCGAGTTATGCG GCTGGTCAAGCTTCTCAGTAAGGGTGAAGGGATCCGCACATTGCTCTGGACATTCATCAAGTCCTTCCAG GCCTTGCCCTATGTGGCTCTTCTCATCGCAATGATATTCTTCATCTATGCCGTCATTGGCATGCAGATGT TCGGCAAGGTGGCTCTTCAGGATGGCACACAGATAAACCGAAACAACAACTTCCAGACCTTTCCACAGGC TGTGCTGCTTCTGTTCAGGTGTGCCACTGGTGAGGCATGGCAGGAGATAATGCTTGCCAGCCTTCCCGGA AATCGGTGTGATCCTGAGTCTGACTTCGGCCCTGGTGAAGAGTTTACCTGTGGTAGCAATTTTGCCATCG CCTATTTCATCAGCTTCTTCATGCTCTGTGCCTTCCTGATCATAAATCTCTTTGTGGCTGTGATCATGGA CAACTTTGATTATCTCACCAGAGATTGGTCCATCCTGGGCCCCCATCACCTTGATGAATTCAAGAGGATC TGGTCTGAATATGACCCTGGGGCCAAGGGCCGCATCAAACACTTGGATGTGGTTGCCCTGCTGAGACGTA TCCAGCCCCCTCTGGGATTTGGGAAGCTGTGCCCACACCGAGTGGCCTGCAAGAGACTTGTGGCAATGAA CATGCCCCTCAACTCAGATGGGACGGTGACATTCAACGCCACACTCTTTGCCCTGGTCCGGACATCCCTG AAGATCAAAACAGAAGGGAACCTGGAGCAAGCCAACCAGGAGCTGCGGATTGTCATCAAAAAGATCTGGA AGCGGATGAAACAGAAGCTGCTAGATGAGGTCATCCCCCCACCAGACGAGGAGGAGGTCACCGTGGGCAA ATTCTACGCCACATTTCTGATCCAGGACTATTTCCGCAAATTCCGGCGGAGGAAAGAAAAAGGGCTACTA GGCAACGACGCCGCCCCTAGCACCTCTTCCGCCCTTCAGGCTGGTCTGCGGAGCCTGCAGGACTTGGGTC CTGAGATGCGGCAGGCCCTCACCTGTGACACAGAGGAGGAGGAAGAAGAGGGGCAGGAGGGAGTGGAGGA GGAAGATGAAAAGGACTTGGAAACTAACAAAGCCACGATGGTCTCCCAGCCCTCAGCTCGCCGGGGCTCC GGGATTTCTGTGTCTCTGCCTGTCGGGGACAGACTTCCAGATTCACTCTCCTTTGGGCCCAGTGATGATG ACAGGGGGACTCCCACCTCCAGTCAGCCCAGTGTGCCCCAGGCTGGATCCAACACCCACAGGAGAGGCTC TGGGGCTCTCATTTTCACCATCCCAGAAGAAGGAAATTCTCAGCCCAAGGGAACCAAAGGGCAAAACAAG CAAGATGAGGATGAGGAAGTCCCTGATCGGCTTTCCTACCTAGATGAGCAGGCAGGGACTCCCCCGTGCT CAGTCCTTTTGCCACCTCACAGAGCTCAGAGATACATGGATGGGCACCTGGTACCACGCCGCCGTCTGCT GCCCCCCACACCTGCAGGTCGGAAGCCCTCCTTCACCATCCAGTGTCTGCAGCGCCAGGGCAGTTGTGAG GATTTACCCATCCCAGGCACCTATCATCGTGGGCGAAATTCAGGGCCCAATAGGGCTCAGGGTTCCTGGG CAACACCACCTCAGCGGGGTCGGCTCCTGTATGCCCCGCTGTTGTTGGTGGAAGAGGGCGCAGCGGGGGA GGGGTACCTCGGCAGATCCAGTGGCCCACTGCGCACCTTCACCTGTCTGCACGTGCCTGGAACCCACTCG GACCCCAGCCATGGGAAGAGGGGCAGTGCCGACAGCTTGGTGGAGGCTGTGCTTATCTCAGAGGGTCTGG GCCTCTTTGCTCGAGACCCACGTTTCGTGGCCCTGGCCAAGCAGGAGATTGCAGATGCGTGTCGCCTGAC GCTGGATGAGATGGACAATGCTGCCAGTGACCTGCTGGCACAGGGAACCAGCTCTCTCTATAGCGACGAG GAGTCCATCCTCTCCCGCTTCGATGAGGAGGACTTGGGAGACGAGATGGCCTGCGTCCACGCCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235349 representing NM_001256790 Red=Cloning site Green=Tags(s) MSESEGGKGERILPSLQTLGASIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFL VIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKAL RAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDME AEEDPSPCASSGSGRACTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGY ELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELD MEDPSADDNLGSMAEEGRAGHRPQLAELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTETQGDE DEEEGALASCTRCLNKIMKTRVCRRLRRANRVLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPV WLTQIQEYANKVLLCLFTVEMLLKLYGLGPSAYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLR CVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFD TFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLASGD AGTAKDKGGEKSNEKDLPQENEGLVPGVEKEEEEGARREGADMEEEEEEEEEEEEEEEEEGAGGVELLQE VVPKEKVVPIPEGSAFFCLSQTNPLRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILG YFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSAISVVKILRVLRVLR PLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFL VYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISV FFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWA TVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWN TFDALIVVGSIVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQ ALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPG NRCDPESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRI WSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSL KIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLL GNDAAPSTSSALQAGLRSLQDLGPEMRQALTCDTEEEEEEGQEGVEEEDEKDLETNKATMVSQPSARRGS GISVSLPVGDRLPDSLSFGPSDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGTKGQNK QDEDEEVPDRLSYLDEQAGTPPCSVLLPPHRAQRYMDGHLVPRRRLLPPTPAGRKPSFTIQCLQRQGSCE DLPIPGTYHRGRNSGPNRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGRSSGPLRTFTCLHVPGTHS DPSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDNAASDLLAQGTSSLYSDE ESILSRFDEEDLGDEMACVHAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256790
ORF Size	5736 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256790.3
RefSeq Size	5875 bp
RefSeq ORF	5739 bp
Locus ID	778
UniProt ID	O60840
Protein Families	Druggable Genome, Ion Channels: Calcium, Transmembrane
Protein Pathways	Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Vascular smooth muscle contraction
MW	214.5 kDa
Gene Summary	This gene encodes a multipass transmembrane protein that functions as an alpha-1 subunit of the voltage-dependent calcium channel, which mediates the influx of calcium ions into the cell. The encoded protein forms a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Mutations in this gene can cause X-linked eye disorders, including congenital stationary night blindness type 2A, cone-rod dystropy, and Aland Island eye disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2013]

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