USP19 (NM_001199162) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ZMYND9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235284 representing NM_001199162 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGGCGGGGCCAGTGCCACAGGCCCAAGGAGAGGGCCCCCAGGACTGGAGGACACCACTAGTAAGA AGAAGCAGAAGGATCGAGCAAACCAGGAGAGCAAGGATGGAGATCCTAGGAAAGAGACAGGGTCTCGATA TGTTGCCCAGGCTGGTCTTGAACCTCTGGCCTCAGGTGATCCTTCTGCCTCAGCCTCCCATGCAGCTGGG ATCACAGGCTCACGCCACCGTACCCGGCTGTTCTTTCCTTCATCGTCAGGGTCAGCATCCACTCCTCAAG AGGAGCAGACCAAAGAGGGAGCTTGTGAAGACCCTCATGATCTCTTGGCTACTCCCACTCCAGAGTTGTT GCTCGATTGGAGGCAGAGTGCAGAAGAGGTGATTGTCAAGCTTCGTGTGGGAGTAGGTCCCCTGCAGCTG GAGGATGTAGATGCTGCTTTCACAGATACAGACTGTGTGGTGCGGTTTGCAGGTGGTCAGCAGTGGGGTG GTGTCTTCTATGCTGAGATAAAAAGCTCTTGTGCTAAAGTGCAAACCCGCAAGGGCAGTCTCCTGCACCT GACACTGCCCAAAAAGAAGAAACCTCTAGGGACCCAGGAGCTGGTGCCGGGGCTGCGGTGCCAGGAGAAT GGGCAGGAACTGTCTCCCATTGCCCTGGAGCCAGGCCCTGAGCCCCACCGGGCTAAGCAGGAGGCCCGGA ACCAGAAGCGGGCCCAGGGCCGTGGTGAGGTAGGCGCAGGGGCTGGCCCCGGGGCCCAGGCAGGGCCCAG CGCCAAGAGGGCTGTGCATCTCTGCAGAGGGCCAGAGGGGGACGGGTCCAGGGATGACCCTGGACCCCGG GGTGATGCCCCACCCTTCGTGGCTGACCCAGCCACCCAGGTTGAGGCTGATGAACAGCTTTGCATACCAC CGCTGAACTCCCAAACCTGCCTCCTGGGCTCAGAGGAGAATTTAGCCCCTTTGGCAGGAGAGAAAGCAGT GCCTCCCGGGAATGACCCAGTCTCTCCAGCCATGGTCCGGAGCAGAAACCCTGGGAAAGATGACTGTGCC AAGGAGGAGATGGCAGTGGCAGCAGATGCTGCAACCTTGGTGGATGAGCCCGAGTCGATGGTGAACCTGG CGTTTGTCAAGAATGACTCGTATGAGAAGGGCCCGGATTCAGTGGTGGTGCACGTGTACGTGAAGGAGAT CTGCAGGGACACCTCAAGAGTACTTTTCCGTGAGCAGGACTTCACGCTCATCTTCCAGACCAGGGATGGA AACTTCCTGAGGCTGCACCCGGGCTGTGGGCCCCACACCACCTTCCGTTGGCAGGTGAAGCTCAGGAATC TGATTGAGCCAGAGCAGTGCACCTTCTGTTTCACGGCTTCTCGCATCGACATCTGCCTTCGTAAGAGGCA GAGTCAGCGCTGGGGGGGCCTGGAGGCCCCGGCTGCACGAGTGGGTGGTGCAAAGGTTGCCGTGCCGACA GGTCCAACCCCTCTGGATTCAACCCCACCAGGAGGTGCTCCCCACCCCCTGACAGGCCAGGAGGAGGCCC GGGCTGTGGAGAAGGATAAATCCAAGGCACGATCTGAGGACACAGGGCTAGACAGTGTGGCAACCCGCAC ACCCATGGAGCATGTAACCCCAAAGCCAGAGACACACCTGGCCTCGCCCAAGCCTACATGCATGGTGCCT CCCATGCCCCACAGCCCAGTTAGTGGAGACAGCGTGGAGGAGGAGGAAGAGGAAGAGAAGAAGGTGTGTC TGCCAGGCTTCACTGGCCTTGTCAATTTAGGCAACACCTGCTTCATGAACAGCGTCATTCAGTCTCTGTC CAACACTCGGGAACTCCGGGACTTCTTCCATGACCGCTCCTTTGAGGCTGAGATCAACTACAACAACCCA CTAGGGACTGGTGGGCGTCTGGCCATTGGCTTTGCCGTGCTGCTTCGGGCGCTGTGGAAGGGCACCCACC ATGCCTTCCAGCCTTCCAAGTTGAAGGCCATTGTGGCGAGTAAGGCCAGCCAGTTCACAGGCTATGCACA GCATGATGCCCAGGAGTTCATGGCTTTCCTGCTGGATGGGCTGCACGAGGACCTGAATCGCATTCAGAAC AAGCCCTACACAGAGACCGTGGATTCAGATGGGCGGCCCGATGAGGTGGTAGCTGAGGAAGCATGGCAGC GGCACAAGATGAGGAATGACTCTTTCATCGTGGACCTATTTCAGGGGCAGTACAAGTCGAAGCTGGTGTG CCCTGTGTGTGCCAAGGTCTCCATCACTTTTGACCCGTTTCTTTATCTGCCGGTGCCCTTGCCACAAAAG CAAAAGGTTCTCCCTGTCTTTTATTTTGCCCGAGAGCCCCACAGCAAGCCCATCAAGTTCCTGGTGAGCG TCAGCAAGGAGAACTCCACTGCGAGCGAAGTATTGGACTCCCTCTCTCAGAGTGTTCATGTGAAGCCTGA GAACCTGCGTTTGGCGGAGGTAATTAAGAATCGTTTTCATCGTGTGTTCCTACCCTCCCACTCACTGGAC ACTGTGTCCCCATCTGATACGCTCCTCTGCTTTGAGCTGCTATCCTCAGAGTTGGCTAAGGAGCGGGTAG TGGTGCTAGAGGTGCAACAGCGCCCCCAGGTGCCCAGCGTCCCCATCTCCAAGTGTGCAGCCTGCCAGCG GAAGCAACAGTCGGAGGATGAAAAGCTGAAGCGCTGTACCCGGTGCTACCGTGTGGGCTACTGCAACCAG CTCTGCCAGAAAACCCACTGGCCTGACCACAAGGGCCTCTGCCGACCTGAGAACATTGGCTACCCCTTCC TGGTCAGTGTACCTGCCTCACGCCTCACTTATGCCCGCCTCGCTCAGTTGCTAGAGGGCTATGCCCGGTA CTCTGTGAGTGTATTCCAGCCACCCTTTCAGCCAGGCCGCATGGCCTTGGAGTCTCAGAGCCCTGGCTGC ACCACACTGCTCTCCACAGGTTCCCTGGAGGCTGGGGACAGCGAGAGAGACCCCATTCAGCCACCTGAGC TCCAGCTGGTGACCCCTATGGCTGAGGGGGACACAGGGCTTCCCCGGGTGTGGGCAGCCCCTGACCGGGG TCCTGTGCCCAGCACCAGTGGAATTTCTTCTGAGATGCTGGCCAGTGGGCCCATTGAGGTTGGCTCCTTG CCAGCTGGCGAGAGGGTGTCCCGACCCGAAGCTGCTGTGCCTGGGTACCAGCATCCAAGTGAAGCTATGA ATGCCCACACACCCCAGTTCTTCATCTATAAAATTGATTCATCCAACCGAGAGCAGCGGCTAGAGGACAA AGGAGACACCCCACTGGAGCTGGGTGACGACTGTAGCCTGGCTCTCGTCTGGCGGAACAATGAGCGCTTG CAGGAGTTTGTGTTGGTAGCCTCCAAGGAGCTGGAATGTGCTGAGGATCCAGGCTCTGCCGGTGAGGCTG CCCGGGCCGGCCACTTCACCCTGGACCAGTGCCTCAACCTCTTCACACGGCCTGAGGTGCTGGCACCCGA GGAGGCCTGGTACTGCCCACAGTGCAAACAGCACCGTGAGGCCTCCAAGCAGCTGTTGCTATGGCGCCTG CCAAATGTTCTCATCGTGCAGCTCAAGCGCTTCTCCTTTCGTAGTTTTATCTGGCGTGACAAGATCAATG ACTTGGTGGAGTTCCCTGTTAGGAACCTGGACCTGAGCAAGTTCTGCATTGGTCAGAAAGAGGAGCAGCT GCCCAGCTACGATCTATATGCTGTCATCAACCACTATGGAGGCATGATTGGTGGCCACTACACTGCCTGT GCACGCCTGCCCAATGATCGTAGCAGTCAGCGCAGTGACGTGGGCTGGCGCTTGTTTGATGACAGCACAG TGACAACGGTAGACGAGAGCCAGGTTGTGACGCGTTATGCCTATGTACTCTTCTACCGCCGGCGGAACTC TCCTGTGGAGAGGCCCCCCAGGGCAGGTCACTCTGAGCACCACCCAGACCTAGGCCCTGCAGCTGAGGCT GCTGCCAGCCAGGGACTAGGCCCTGGCCAGGCCCCCGAGGTGGCCCCCACGCGGACAGCCCCTGAACGCT TCGCCCCCCCTGTGGATCGGCCAGCCCCCACCTACAGCAACATGGAGGAGGTGGAT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235284 representing NM_001199162 Red=Cloning site Green=Tags(s) MSGGASATGPRRGPPGLEDTTSKKKQKDRANQESKDGDPRKETGSRYVAQAGLEPLASGDPSASASHAAG ITGSRHRTRLFFPSSSGSASTPQEEQTKEGACEDPHDLLATPTPELLLDWRQSAEEVIVKLRVGVGPLQL EDVDAAFTDTDCVVRFAGGQQWGGVFYAEIKSSCAKVQTRKGSLLHLTLPKKKKPLGTQELVPGLRCQEN GQELSPIALEPGPEPHRAKQEARNQKRAQGRGEVGAGAGPGAQAGPSAKRAVHLCRGPEGDGSRDDPGPR GDAPPFVADPATQVEADEQLCIPPLNSQTCLLGSEENLAPLAGEKAVPPGNDPVSPAMVRSRNPGKDDCA KEEMAVAADAATLVDEPESMVNLAFVKNDSYEKGPDSVVVHVYVKEICRDTSRVLFREQDFTLIFQTRDG NFLRLHPGCGPHTTFRWQVKLRNLIEPEQCTFCFTASRIDICLRKRQSQRWGGLEAPAARVGGAKVAVPT GPTPLDSTPPGGAPHPLTGQEEARAVEKDKSKARSEDTGLDSVATRTPMEHVTPKPETHLASPKPTCMVP PMPHSPVSGDSVEEEEEEEKKVCLPGFTGLVNLGNTCFMNSVIQSLSNTRELRDFFHDRSFEAEINYNNP LGTGGRLAIGFAVLLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHEDLNRIQN KPYTETVDSDGRPDEVVAEEAWQRHKMRNDSFIVDLFQGQYKSKLVCPVCAKVSITFDPFLYLPVPLPQK QKVLPVFYFAREPHSKPIKFLVSVSKENSTASEVLDSLSQSVHVKPENLRLAEVIKNRFHRVFLPSHSLD TVSPSDTLLCFELLSSELAKERVVVLEVQQRPQVPSVPISKCAACQRKQQSEDEKLKRCTRCYRVGYCNQ LCQKTHWPDHKGLCRPENIGYPFLVSVPASRLTYARLAQLLEGYARYSVSVFQPPFQPGRMALESQSPGC TTLLSTGSLEAGDSERDPIQPPELQLVTPMAEGDTGLPRVWAAPDRGPVPSTSGISSEMLASGPIEVGSL PAGERVSRPEAAVPGYQHPSEAMNAHTPQFFIYKIDSSNREQRLEDKGDTPLELGDDCSLALVWRNNERL QEFVLVASKELECAEDPGSAGEAARAGHFTLDQCLNLFTRPEVLAPEEAWYCPQCKQHREASKQLLLWRL PNVLIVQLKRFSFRSFIWRDKINDLVEFPVRNLDLSKFCIGQKEEQLPSYDLYAVINHYGGMIGGHYTAC ARLPNDRSSQRSDVGWRLFDDSTVTTVDESQVVTRYAYVLFYRRRNSPVERPPRAGHSEHHPDLGPAAEA AASQGLGPGQAPEVAPTRTAPERFAPPVDRPAPTYSNMEEVD SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199162
ORF Size	4116 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199162.1, NP_001186091.1
RefSeq Size	4847 bp
RefSeq ORF	4119 bp
Locus ID	10869
UniProt ID	O94966
Protein Families	Druggable Genome, Protease, Transmembrane
MW	151.1 kDa
Gene Summary	Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This protein is a ubiquitin protein ligase and plays a role in muscle wasting. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2017]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.