PASK (NM_001252122) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PASKIN; STK37
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235255 representing NM_001252122 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACGGGGGCTTAACAGCCTTTGAAGAGGACCAGAGATGCCTTTCCCAGAGCCTCCCCTTGCCAG TGTCAGCAGAGGGCCCAGCTGCACAGACCACTGCTGAGCCCAGCAGGTCGTTTTCCTCAGCCCACAGACA CCTGAGCAGAAGGAATGGGCTTTCCAGACTCTGCCAGAGCAGGACAGCGCTCTCTGAAGACAGATGGAGC TCCTATTGTCTATCATCACTGGCTGCCCAGAATATTTGTACAAGTAAACTGCACTGCCCTGCTGCCCCTG AGCACACGGACCCGTCCGAACCGCGGGGCAGTGTGTCCTGCTGCTCCCTGCTGCGGGGACTGTCCTCAGG GTGGTCCTCACCTCTGCTTCCGGCCCCTGTGTGCAACCCTAACAAGGCCATCTTCACGGTGGATGCCAAG ACCACAGAGATCCTGGTTGCTAACGACAAAGCTTGCGGGCTCCTGGGGTACAGCAGCCAGGACCTGATTG GCCAGAAGCTCACGCAGTTCTTTCTGAGGTCAGATTCTGATGTGGTGGAGGCCCTCAGCGAGGAGCACAT GGAGGCCGACGGCCACGCTGCGGTGGTGTTTGGCACGGTGGTGGACATCATCAGCCGTAGTGGGGAGAAG ATTCCAGTGTCTGTGTGGATGAAGAGGATGCGGCAGGAGCGCCGCCTATGCTGCGTGGTGGTCCTGGAGC CCGTGGAGAGGGTCTCGACCTGGGTCGCTTTCCAGAGCGATCTCCCTCCTTCTGGCCAGCACATCCCAAA GAATCTCAAGATTCAGAGGTCTGTTGGAAGAGCCAGGGACGGTACCACCTTCCCTCTGAGCTTAAAGCTG AAATCCCAACCCAGCAGCGAGGAGGCGACCACCGGTGAGGCGGCCCCTGTGAGCGGCTACCGGGCATCTG TCTGGGTGTTCTGCACCATCAGTGGCCTCATCACCCTCCTGCCGGATGGGACCATCCACGGCATCAACCA CAGCTTCGCGCTGACACTGTTTGGTTACGGAAAGACGGAGCTCCTGGGCAAGAATATCACTTTCCTGATT CCTGGTTTCTACAGCTACATGGACCTTGCGTACAACAGCTCATTACAGCTCCCAGACCTGGCCAGCTGCC TGGACGTCGGCAATGAGAGTGGGTGTGGGGAGAGAACCTTGGACCCGTGGCAGGGCCAGGACCCAGCTGA GGGGGGCCAGGATCCAAGGATTAATGTCGTGCTTGCTGGTGGCCACGTTGTGCCCCGAGATGAGATCCGG AAGCTGATGGAAAGCCAAGACATCTTCACCGGGACTCAGACTGAGCTGATTGCTGGAGGCCAGCTCCTTT CCTGCCTCTCACCTCAGCCTGCTCCAGGGGTGGACAATGTCCCAGAAGGAAGCCTGCCAGTGCACGGTGA ACAGGCGCTGCCCAAGGACCAGCAAATCACTGCCTTGGGGAGAGAGGAACCTGTGGCAATAGAGAGCCCC GGACAGGATCTTCTGGGAGAAAGCAGGTCTGAACCAGTGGATGTGAAGCCATTTGCTTCCTGCGAAGATT CTGAAGCTCCAGTCCCAGCTGAGGATGGGGGCAGTGATGCTGGCATGTGTGGCCTGTGTCAGAAGGCCCA GCTAGAGCGGATGGGAGTCAGTGGTCCCAGCGGTTCAGACCTTTGGGCTGGGGCTGCCGTGGCCAAGCCC CAGGCCAAGGGTCAGCTGGCGGGGGGCAGCCTCCTGATGCACTGCCCTTGCTATGGGAGTGAATGGGGCT TGTGGTGGCGAAGCCAGGACTTGGCCCCCAGCCCCTCTGGGATGGCAGGCCTCTCGTTTGGGACACCTAC TCTAGATGAGCCGTGGCTGGGAGTGGAAAACGACCGAGAAGAGCTGCAGACCTGCTTGATTAAGGAGCAG CTGTCCCAGTTGAGCCTTGCAGGAGCCCTGGATGTCCCCCACGCCGAACTCGTTCCGACAGAGTGCCAGG CTGTCACCGCTCCTGTGTCGTCCTGCGATCTGGGAGGCAGAGACCTGTGCGGTGGCTGCACGGGCAGCTC CTCAGCCTGCTATGCCTTGGCCACGGACCTCCCTGGGGGCCTGGAAGCAGTGGAGGCCCAGGAGGTTGAT GTGAATTCGTTTTCCTGGAACCTCAAGGAACTCTTTTTCAGTGACCAGACAGACCAAACGTCATCAAATT GTTCCTGTGCTACGTCTGAACTCAGAGAGACACCCTCTTCCTTGGCAGTGGGCTCCGATCCAGATGTAGG CAGTCTCCAGGAACAGGGGTCGTGTGTCCTGGATGACAGGGAGCTGTTACTACTGACCGGCACCTGTGTT GACCTTGGCCAAGGCCGACGGTTCCGGGAGAGCTGTGTGGGACATGATCCAACAGAACCGCTTGAGGTTT GTTTGGTGTCCTCTGAGCATTATGCAGCAAGCGACAGAGAAAGCCCAGGACACGTTCCTTCCACGTTGGA TGCTGGCCCTGAGGACACGTGCCCATCAGCAGAGGAGCCAAGGCTGAACGTCCAGGTCACCTCCACGCCC GTGATCGTGATGCGCGGGGCTGCTGGCCTGCAGCGGGAGATCCAGGAGGGTGCCTACTCCGGGAGCTGCT ACCATCGAGATGGCTTACGGCTGAGTATACAGTTTGAGGTGAGGCGGGTGGAGCTCCAGGGCCCCACACC TCTGTTCTGCTGCTGGCTGGTGAAAGACCTCCTCCACAGCCAACGCGACTCAGCCGCCAGGACCCGCCTG TTCCTTGCCAGCCTGCCCGGCTCCACCCACTCTACCGCTGCTGAGCTCACCGGACCCAGCCTGGTGGAAG TGCTCAGAGCCAGACCCTGGTTTGAGGAGCCCCCCAAGGCTGTGGAACTGGAGGGGTTGGCGGCCTGTGA GGGCGAGTACTCCCAAAAGTACAGTACCATGAGCCCGCTGGGCAGTGGGGCCTTCGGCTTCGTGTGGACT GCTGTGGACAAGGAAAAAAACAAGGAGGTGGTGGTGAAGTTTATTAAGAAGGAGAAGGTCTTGGAGGATT GTTGGATTGAGGATCCCAAACTTGGGAAAGTTACTTTAGAGATCGCAATTCTATCCAGGGTGGAGCACGC CAATATCATCAAGGTATTGGATATATTTGAAAACCAAGGGTTCTTCCAGCTTGTGATGGAGAAGCACGGC TCCGGCCTAGACCTCTTCGCTTTCATCGACCGCCACCCCAGGCTGGATGAGCCCCTGGCGAGCTACATCT TCCGACAACTAGTGTCAGCAGTGGGATACCTGCGCTTGAAGGACATCATCCACCGTGACATCAAGGATGA GAACATCGTGATCGCCGAGGACTTCACAATCAAGCTGATAGACTTTGGCTCGGCCGCCTACTTGGAAAGG GGAAAATTATTTTATACTTTTTGTGGGACCATCGAGTACTGTGCACCGGAAGTTCTCATGGGGAATCCCT ACAGAGGGCCGGAGCTGGAGATGTGGTCTCTGGGAGTCACTCTGTACACGCTGGTCTTTGAGGAGAACCC CTTCTGTGAGCTGGAGGAGACCGTGGAGGCTGCCATACACCCGCCATACCTGGTGTCCAAAGAACTCATG AGCCTTGTGTCTGGGCTGCTGCAGCCAGTCCCTGAGAGACGCACCACCTTGGAGAAGCTGGTGACAGACC CGTGGGTAACACAGCCTGTGAATCTTGCTGACTATACATGGGAAGAGGTGTTTCGAGTAAACAAGCCAGA AAGTGGAGTTCTGTCCGCTGCGAGCCTGGAGATGGGGAACAGGAGCCTGAGTGATGTGGCCCAGGCTCAG GAGCTTTGTGGGGGCCCCGTTCCAGGCGAGGCTCCTAATGGCCAAGGCTGTTTGCATCCCGGGGATCCCC GTCTGCTGACCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235255 representing NM_001252122 Red=Cloning site Green=Tags(s) MEDGGLTAFEEDQRCLSQSLPLPVSAEGPAAQTTAEPSRSFSSAHRHLSRRNGLSRLCQSRTALSEDRWS SYCLSSLAAQNICTSKLHCPAAPEHTDPSEPRGSVSCCSLLRGLSSGWSSPLLPAPVCNPNKAIFTVDAK TTEILVANDKACGLLGYSSQDLIGQKLTQFFLRSDSDVVEALSEEHMEADGHAAVVFGTVVDIISRSGEK IPVSVWMKRMRQERRLCCVVVLEPVERVSTWVAFQSDLPPSGQHIPKNLKIQRSVGRARDGTTFPLSLKL KSQPSSEEATTGEAAPVSGYRASVWVFCTISGLITLLPDGTIHGINHSFALTLFGYGKTELLGKNITFLI PGFYSYMDLAYNSSLQLPDLASCLDVGNESGCGERTLDPWQGQDPAEGGQDPRINVVLAGGHVVPRDEIR KLMESQDIFTGTQTELIAGGQLLSCLSPQPAPGVDNVPEGSLPVHGEQALPKDQQITALGREEPVAIESP GQDLLGESRSEPVDVKPFASCEDSEAPVPAEDGGSDAGMCGLCQKAQLERMGVSGPSGSDLWAGAAVAKP QAKGQLAGGSLLMHCPCYGSEWGLWWRSQDLAPSPSGMAGLSFGTPTLDEPWLGVENDREELQTCLIKEQ LSQLSLAGALDVPHAELVPTECQAVTAPVSSCDLGGRDLCGGCTGSSSACYALATDLPGGLEAVEAQEVD VNSFSWNLKELFFSDQTDQTSSNCSCATSELRETPSSLAVGSDPDVGSLQEQGSCVLDDRELLLLTGTCV DLGQGRRFRESCVGHDPTEPLEVCLVSSEHYAASDRESPGHVPSTLDAGPEDTCPSAEEPRLNVQVTSTP VIVMRGAAGLQREIQEGAYSGSCYHRDGLRLSIQFEVRRVELQGPTPLFCCWLVKDLLHSQRDSAARTRL FLASLPGSTHSTAAELTGPSLVEVLRARPWFEEPPKAVELEGLAACEGEYSQKYSTMSPLGSGAFGFVWT AVDKEKNKEVVVKFIKKEKVLEDCWIEDPKLGKVTLEIAILSRVEHANIIKVLDIFENQGFFQLVMEKHG SGLDLFAFIDRHPRLDEPLASYIFRQLVSAVGYLRLKDIIHRDIKDENIVIAEDFTIKLIDFGSAAYLER GKLFYTFCGTIEYCAPEVLMGNPYRGPELEMWSLGVTLYTLVFEENPFCELEETVEAAIHPPYLVSKELM SLVSGLLQPVPERRTTLEKLVTDPWVTQPVNLADYTWEEVFRVNKPESGVLSAASLEMGNRSLSDVAQAQ ELCGGPVPGEAPNGQGCLHPGDPRLLTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252122
ORF Size	3864 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252122.2
RefSeq Size	4483 bp
RefSeq ORF	3867 bp
Locus ID	23178
UniProt ID	Q96RG2
Protein Families	Druggable Genome, Protein Kinase, Stem cell - Pluripotency
MW	139.7 kDa
Gene Summary	This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

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