UTY (NM_001258250) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	KDM6AL; KDM6C; UTY1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235215 representing NM_001258250 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCCTGCGCAGTGTCGCTCACTACCGCCGCTGTTGCCTTCGGTGATGAGGCAAAGAAAATGGCGG AAGGAAAAGCGAGCCGCGAGAGTGAAGAGGAGTCTGTTAGCCTGACAGTCGAGGAAAGGGAGGCGCTTGG TGGCATGGACAGCCGTCTCTTCGGGTTCGTGAGGCTTCATGAAGATGGCGCCAGAACGAAGACCCTACTA GGCAAGGCTGTTCGCTGCTACGAATCTTTAATCTTAAAAGCTGAAGGAAAAGTGGAGTCTGACTTCTTTT GCCAATTAGGTCACTTCAACCTCTTGTTGGAAGATTATTCAAAAGCATTATCTGCATATCAGAGATATTA CAGTTTACAGGCTGACTACTGGAAGAATGCTGCGTTTTTATATGGCCTTGGTTTGGTCTACTTCTACTAC AATGCATTTCATTGGGCAATTAAAGCATTTCAAGATGTCCTTTATGTTGACCCCAGCTTTTGTCGAGCCA AGGAAATTCATTTACGACTTGGGCTCATGTTCAAAGTGAACACAGACTACAAGTCTAGTTTAAAGCATTT TCAGTTAGCCTTGATTGACTGTAATCCATGTACTTTGTCCAATGCTGAAATTCAATTTCATATTGCCCAT TTGTATGAAACCCAGAGGAAGTATCATTCTGCAAAGGAGGCATATGAACAACTTTTGCAGACAGAAAACC TTCCTGCACAAGTAAAAGCAACTGTATTGCAACAGTTAGGTTGGATGCATCATAATATGGATCTAGTAGG AGACAAAGCCACAAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCTTTGGAGGCAGATCCTAATTCT GGCCAATCGTGGTATTTTCTTGGAAGGCAATCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTT CAATAGGTGTGTTGTATCAGCAGCAAAATCAGCCTATGGATGCTTTACAGGCATATATTTGTGCTGTACA ATTGGACCATGGGCATGCCGCAGCCTGGATGGACCTAGGTACTCTCTATGAATCCTGCAATCAACCTCAA GATGCCATTAAATGCTACCTAAATGCAGCTAGAAGCAAACGTTGTAGTAATACCTCTACGCTTGCTGCAA GAATTAAATTTCTACAGAATGGTTCTGATAACTGGAATGGTGGCCAGAGTCTTTCACATCATCCAGTACA GCAAGTTTATTCGTTGTGTTTGACACCACAGAAATTACAGCACTTGGAACAACTGCGAGCAAATAGAGAT AATTTAAATCCAGCACAGAAGCATCAGCTGGAACAGTTAGAAAGTCAGTTTGTCTTAATGCAGCAAATGA GACACAAAGAAGTTGCTCAGGGGCTTCATAAAAGTCAGAGTTCATGTTTGTCAGGACCTAATGAAGAACA ACCTCTGTTTTCCACTGGGTCAGCCCAGTATCACCAGGCAACTAGCACTGGTATTAAGAAGGCGAATGAA CATCTCACTCTGCCTAGTAATTCAGTACCACAGGGGGATGCTGACAGTCACCTCTCCTGTCATACTGCTA CCTCAGGTGGACAACAAGGCATTATGTTTACCAAAGAGAGCAAGCCTTCAAAAAATAGATCCTTGGTGCC TGAAACAAGCAGGCATACTGGAGACACATCTAATGGCTGTGCTGATGTCAAGGGACTTTCTAATCATGTT CATCAGTTGATAGCAGATGCTGTTTCCAGTCCTAACCATGGAGATTCACCAAATTTATTAATTGCAGACA ATCCTCAGCTCTCTGCTTTGTTGATTGGAAAAGCCAATGGCAATGTGGGTACTGGAACCTGTGACAAAGT GAATAATATTCACCCAGCTGTTCATACAAAGACTGATCATTCTGTTGCCTCTTCACCCTCTTCAGCCATT TCCACAGCAACACCTTCTCCTAAATCCACTGAGCAGAGAAGCATAAACAGTGTTACCAGCCTTAACAGTC CTCACAGTGGATTACACACAGTCAATGGAGAGGGGCTGGGGAAGTCACAGAGCTCTACAAAAGTAGACCT GCCTTTAGCTAGCCACAGATCTACTTCTCAGATCTTACCATCAATGTCAGTGTCTATATGCCCCAGTTCA ACAGAAGTTCTGAAAGCATGCAGGAATCCAGGTAAAAATGGCTTGTCTAATAGCTGCATTTTGTTAGATA AATGTCCACCTCCAAGACCACCAACTTCACCATACCCACCCTTGCCAAAGGACAAGTTGAATCCACCCAC ACCTAGTATTTACTTGGAAAATAAACGTGATGCTTTCTTTCCTCCATTACATCAATTTTGTACAAATCCA AAAAACCCTGTTACAGTAATACGTGGCCTTGCTGGAGCTCTTAAATTAGATCTTGGACTTTTCTCTACCA AAACTTTGGTAGAAGCTAACAATGAACATATGGTAGAAGTGAGGACACAGTTGCTGCAACCAGCAGATGA AAACTGGGATCCCACTGGAACAAAGAAAATCTGGCGTTGTGAAAGCAATAGATCTCATACTACAATTGCC AAATACGCACAATACCAGGCTTCCTCCTTCCAGGAATCATTGAGAGAAGAAAATGAGAAAAGAACACAAC ACAAAGATCATTCAGATAACGAATCCACATCTTCAGAGAATTCTGGAAGGAGAAGGAAAGGACCTTTTAA AACCATAAAATTTGGGACCAACATTGACCTCTCTGATAACAAAAAGTGGAAGTTGCAGTTACATGAACTG ACTAAACTTCCTGCTTTTGCGCGTGTGGTGTCAGCAGGAAATCTTCTAACCCATGTTGGGCATACCATTC TGGGCATGAATACAGTACAACTGTATATGAAAGTTCCAGGGAGTCGGACACCAGGTCACCAAGAAAATAA CAACTTCTGCTCTGTTAACATAAATATTGGTCCAGGAGATTGTGAATGGTTTGTTGTACCTGAAGATTAT TGGGGTGTTCTGAATGACTTCTGTGAAAAAAATAATTTGAATTTTTTAATGAGTTCTTGGTGGCCCAACC TTGAAGATCTTTATGAAGCAAATGTCCCTGTGTATAGATTTATTCAGCGACCTGGAGATTTGGTCTGGAT AAATGCAGGCACTGTGCATTGGGTTCAAGCTGTTGGCTGGTGCAATAACATTGCCTGGAATGTTGGTCCA CTTACAGCCTGCCAGTATAAATTGGCAGTGGAACGGTATGAATGGAACAAATTGAAAAGTGTGAAGTCAC CAGTACCCATGGTGCATCTTTCCTGGAATATGGCACGAAATATCAAAGTCTCAGATCCAAAGCTTTTTGA AATGATTAAGTATTGTCTTTTGAAAATTCTGAAGCAATATCAGACATTGAGAGAAGCTCTTGTTGCAGCA GGAAAAGAGGTTATATGGCATGGGCGGACAAATGATGAACCAGCTCATTACTGTAGCATTTGTGAGGTGG AGGTTTTTAATCTGCTTTTTGTCACTAATGAAAGCAATACTCAAAAAACCTACATAGTACATTGCCATGA TTGTGCACGAAAAACAAGCAAAAGTTTGGAAAATTTTGTGGTGCTCGAACAGTACAAAATGGAGGACCTA ATCCAAGTTTATGATCAATTTACACTAGCTCTTTCATTATCATCCTCATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235215 representing NM_001258250 Red=Cloning site Green=Tags(s) MKSCAVSLTTAAVAFGDEAKKMAEGKASRESEEESVSLTVEEREALGGMDSRLFGFVRLHEDGARTKTLL GKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYSKALSAYQRYYSLQADYWKNAAFLYGLGLVYFYY NAFHWAIKAFQDVLYVDPSFCRAKEIHLRLGLMFKVNTDYKSSLKHFQLALIDCNPCTLSNAEIQFHIAH LYETQRKYHSAKEAYEQLLQTENLPAQVKATVLQQLGWMHHNMDLVGDKATKESYAIQYLQKSLEADPNS GQSWYFLGRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQ DAIKCYLNAARSKRCSNTSTLAARIKFLQNGSDNWNGGQSLSHHPVQQVYSLCLTPQKLQHLEQLRANRD NLNPAQKHQLEQLESQFVLMQQMRHKEVAQGLHKSQSSCLSGPNEEQPLFSTGSAQYHQATSTGIKKANE HLTLPSNSVPQGDADSHLSCHTATSGGQQGIMFTKESKPSKNRSLVPETSRHTGDTSNGCADVKGLSNHV HQLIADAVSSPNHGDSPNLLIADNPQLSALLIGKANGNVGTGTCDKVNNIHPAVHTKTDHSVASSPSSAI STATPSPKSTEQRSINSVTSLNSPHSGLHTVNGEGLGKSQSSTKVDLPLASHRSTSQILPSMSVSICPSS TEVLKACRNPGKNGLSNSCILLDKCPPPRPPTSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNP KNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWRCESNRSHTTIA KYAQYQASSFQESLREENEKRTQHKDHSDNESTSSENSGRRRKGPFKTIKFGTNIDLSDNKKWKLQLHEL TKLPAFARVVSAGNLLTHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEDY WGVLNDFCEKNNLNFLMSSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAVGWCNNIAWNVGP LTACQYKLAVERYEWNKLKSVKSPVPMVHLSWNMARNIKVSDPKLFEMIKYCLLKILKQYQTLREALVAA GKEVIWHGRTNDEPAHYCSICEVEVFNLLFVTNESNTQKTYIVHCHDCARKTSKSLENFVVLEQYKMEDL IQVYDQFTLALSLSSSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258250
ORF Size	3621 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258250.1, NP_001245179.1
RefSeq Size	6106 bp
RefSeq ORF	3624 bp
Locus ID	7404
Protein Families	Transmembrane
MW	135.3 kDa
Gene Summary	This gene encodes a protein containing tetratricopeptide repeats which are thought to be involved in protein-protein interactions. The encoded protein is also a minor histocompatibility antigen which may induce graft rejection of male stem cell grafts. A large number of alternatively spliced transcripts have been observed for this gene, but the full length nature of some of these variants has not been determined. [provided by RefSeq, Apr 2012]

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