DIAPH3 (NM_001258366) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AN; AUNA1; DIA2; diap3; DRF3; mDia2; NSDAN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235208 representing NM_001258366 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACGGCACCAGCCGCGGCTGCACCACCCGGCCCAAGGCTCAGCCGCTGGGACTCCCTACCCTTCCT CAGCCTCTCTCCGCGGCTGCCGGGAAAGCAAGATGCCGCGCAGGAAGGGCCCCCAACACCCTCCGCCGCC CAGTGGCCCCGAGGAGCCTGGGGAGAAGCGCCCCAAGTTTCTGGACAAATTTGCCAGCATAAGAATTCCA GGGAGCAAGAAAGAGAGACCTCCACTTCCCAACCTGAAGACTGCATTTGCAAGCAGTGATTGCTCAGCAG CACCTTTAGAGATGATGGAGAACTTTCCAAAGCCACTGTCAGAGAATGAACTCTTAGAACTTTTTGAAAA AATGATGGAAGATATGAATTTAAATGAAGATAAAAAGGCACCATTGCGGGAAAAGGACTTCAGTATCAAA AAAGAAATGGTGATGCAGTACATTAATACTGCTTCTAAGACAGGAAGTCTTAAGAGAAGCCGACAGATCT CACCTCAGGAATTCATTCATGAGCTGAAAATGGGGTCTGCAGATGAGAGACTTGTCACATGCCTGGAGTC TCTCCGAGTGTCTTTGACCAGCAATCCTGTGAGTTGGGTGGAAAGCTTTGGACATGAAGGGCTTGGATTA TTATTAGACATTTTGGAAAAACTGATTAGTGGAAAAATCCAAGAAAAAGTTGTAAAGAAAAATCAACATA AAGTCATACAGTGTCTAAAAGCCCTGATGAATACGCAGTATGGCTTGGAAAGAATTATGAGTGAGGAGAG GAGCCTTTCCTTATTGGCCAAAGCCGTGGATCCCAGACACCCCAATATGATGACAGATGTGGTTAAACTT CTCTCTGCGGTATGCATTGTAGGGGAAGAAAGCATCCTTGAAGAAGTTTTAGAAGCTTTAACTTCAGCTG GTGAAGAAAAAAAAATTGACAGATTTTTTTGTATTGTGGAAGGCCTCCGGCACAATTCAGTTCAACTGCA AGTAGCTTGTATGCAGCTCATCAATGCCCTGGTTACATCTCCTGATGATTTGGATTTCAGGCTTCACATC AGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAATTTAAAATGCATTAAGAATGATGGCC TGGATATCCAACTTAAAGTCTTTGATGAGCATAAAGAAGAAGATTTGTTTGAGTTATCCCATCGCCTTGA AGATATTAGAGCTGAACTTGATGAAGCATATGATGTTTACAACATGGTGTGGAGCACAGTTAAAGAAACT AGAGCAGAGGGATATTTTATTTCTATTCTTCAGCATCTTTTGCTGATTCGAAATGATTATTTTATAAGGC AACAATACTTCAAATTAATTGATGAGTGTGTATCCCAGATTGTATTGCATAGAGATGGAATGGATCCAGA CTTCACATATCGAAAAAGACTAGATTTAGATTTAACCCAGTTTGTAGACATTTGCATAGATCAAGCAAAA CTAGAAGAGTTTGAAGAGAAAGCATCAGAACTTTACAAGAAATTTGAAAAAGAGTTTACCGACCACCAAG AAACTCAGGCTGAATTGCAGAAAAAAGAGGCAAAGATTAATGAGCTTCAAGCAGAGCTACAAGCTTTTAA GTCTCAGTTTGGTGCCTTGCCAGCTGATTGTAATATTCCTTTGCCTCCCTCTAAAGAAGGTGGAACTGGC CACTCAGCACTTCCTCCTCCGCCTCCACTGCCTTCTGGTGGAGGGGTGCCGCCTCCACCTCCTCCCCCAC CACCTCCTCCACTTCCAGGAATGCGGATGCCATTCAGTGGTCCTGTGCCTCCACCACCTCCCCTGGGATT CCTTGGAGGACAAAATTCTCCTCCTCTACCAATCCTGCCATTTGGGTTGAAACCAAAGAAAGAATTTAAA CCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATCAGACCTCATGAAATGACTGAAAACTGTTTCT GGATAAAAGTAAATGAAAATAAGTATGAAAACGTGGATTTGCTTTGTAAACTTGAGAATACATTTTGTTG CCAACAAAAAGAGAGAAGAGAAGAGGAAGATATTGAAGAGAAGAAATCGATTAAGAAAAAAATTAAAGAA CTTAAGTTTTTAGATTCTAAAATTGCCCAGAACCTTTCAATCTTCCTGAGCTCTTTTCGGGTGCCATATG AGGAAATCAGAATGATGATATTGGAAGTAGATGAAACACGGTTGGCAGAGTCTATGATTCAGAACTTAAT AAAGCATCTTCCTGATCAAGAGCAATTAAATTCATTGTCTCAGTTCAAGAGTGAATATAGCAACTTATGT GAACCTGAGCAGTTTGTGGTTGTGATGAGCAATGTGAAGAGACTACGGCCACGGCTCAGTGCTATTCTCT TTAAGCTTCAGTTTGAAGAGCAGGTGAACAACATCAAACCTGACATCATGGCTGTCAGTACTGCCTGCGA AGAGATAAAGAAGAGCAAAAGCTTTAGCAAGTTGCTGGAACTTGTATTGCTAATGGGAAACTACATGAAT GCTGGCTCCCGGAATGCTCAAACCTTCGGATTTAACCTTAGCTCTCTCTGTAAACTAAAGGACACAAAAT CAGCAGATCAGAAAACAACGCTACTTCATTTCCTGGTAGAAATATGTGAAGAGAAGTACCCTGATATACT GAATTTTGTGGATGATTTGGAACCTTTAGACAAAGCTAGTAAAGTCTCTGTAGAAACGCTGGAAAAGAAT TTGAGGCAGATGGGAAGGCAGCTTCAACAGCTTGAGAAGGAATTGGAAACCTTTCCCCCTCCTGAGGACT TGCATGACAAGTTTGTGACAAAGATGTCCAGATTTGTTATCAGTGCAAAAGAACAATATGAGACACTTTC GAAGTTACACGAAAACATGGAAAAGTTATACCAGAGTATAATAGGATACTATGCCATTGATGTGAAGAAG GTGTCTGTGGAAGACTTTCTTACTGACCTGAATAACTTCAGAACCACATTCATGCAAGCAATAAAGGAGA ATATCAAAAAAAGAGAAGCAGAGGAAAAAGAAAAACGTGTCAGAATAGCTAAAGAATTAGCAGAGCGAGA AAGACTCGAACGCCAACAAAAGAAAAAGCGTTTATTAGAAATGAAGACTGAGGGTGATGAGACAGGAGTG ATGGATAATCTGCTGGAGGCCTTGCAGTCCGGGGCTGCCTTCCGCGACAGAAGAAAAAGGACACCGATGC CAAAAGATGTTCGGCAGAGTCTCAGTCCAATGTCTCAGAGGCCTGTTCTGAAAGTTTGTAACCATGAAAA TCAGAAAGTGCAGTTGACAGAAGGGTCACGTTCACACTACAATATCAATTGCAACTCAACAAGGACTCCA GTCGCCAAGGAGCTTAATTATAATCTAGACACTCATACGTCTACTGGGAGGATCAAGGCAGCTGAGAAGA AGGAAGCGTGTAATGTAGAAAGCAACAGAAAAAAGGAAACGGAACTTCTTGGCTCTTTTTCTAAAAATGA ATCAGTTCCCGAAGTTGAAGCCCTGCTGGCAAGATTACGAGCTTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235208 representing NM_001258366 Red=Cloning site Green=Tags(s) MERHQPRLHHPAQGSAAGTPYPSSASLRGCRESKMPRRKGPQHPPPPSGPEEPGEKRPKFLDKFASIRIP GSKKERPPLPNLKTAFASSDCSAAPLEMMENFPKPLSENELLELFEKMMEDMNLNEDKKAPLREKDFSIK KEMVMQYINTASKTGSLKRSRQISPQEFIHELKMGSADERLVTCLESLRVSLTSNPVSWVESFGHEGLGL LLDILEKLISGKIQEKVVKKNQHKVIQCLKALMNTQYGLERIMSEERSLSLLAKAVDPRHPNMMTDVVKL LSAVCIVGEESILEEVLEALTSAGEEKKIDRFFCIVEGLRHNSVQLQVACMQLINALVTSPDDLDFRLHI RNEFMRCGLKEILPNLKCIKNDGLDIQLKVFDEHKEEDLFELSHRLEDIRAELDEAYDVYNMVWSTVKET RAEGYFISILQHLLLIRNDYFIRQQYFKLIDECVSQIVLHRDGMDPDFTYRKRLDLDLTQFVDICIDQAK LEEFEEKASELYKKFEKEFTDHQETQAELQKKEAKINELQAELQAFKSQFGALPADCNIPLPPSKEGGTG HSALPPPPPLPSGGGVPPPPPPPPPPPLPGMRMPFSGPVPPPPPLGFLGGQNSPPLPILPFGLKPKKEFK PEISMRRLNWLKIRPHEMTENCFWIKVNENKYENVDLLCKLENTFCCQQKERREEEDIEEKKSIKKKIKE LKFLDSKIAQNLSIFLSSFRVPYEEIRMMILEVDETRLAESMIQNLIKHLPDQEQLNSLSQFKSEYSNLC EPEQFVVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKSFSKLLELVLLMGNYMN AGSRNAQTFGFNLSSLCKLKDTKSADQKTTLLHFLVEICEEKYPDILNFVDDLEPLDKASKVSVETLEKN LRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKK VSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEKRVRIAKELAERERLERQQKKKRLLEMKTEGDETGV MDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMSQRPVLKVCNHENQKVQLTEGSRSHYNINCNSTRTP VAKELNYNLDTHTSTGRIKAAEKKEACNVESNRKKETELLGSFSKNESVPEVEALLARLRAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258366
ORF Size	3546 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258366.2
RefSeq Size	4779 bp
RefSeq ORF	3549 bp
Locus ID	81624
UniProt ID	Q9NSV4
Protein Pathways	Regulation of actin cytoskeleton
MW	136.1 kDa
Gene Summary	This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

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