KCNMA1 (NM_001271518) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bA205K10.1; BKTM; CADEDS; hSlo; IEG16; KCa1.1; LIWAS; MaxiK; mSLO1; PNKD3; SAKCA; SLO; SLO-ALPHA; SLO1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235178 representing NM_001271518 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAATGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGAGGCAGCAGTCTTA GAATGAGTAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCCTCCTCCTCTTC CTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATC ATCCCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCCTGGCCTCCT CCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT GTGCTGCCACTGCGGGGGCAAGACGAAGGTTGTCTTAGTCTTTGCTCTCAGCATCGGTGCACTTGTAATA TACTTCATAGATTCATCAAACCCAATAGAATCCTGCCAGAATTTCTACAAAGATTTCACATTACAGATCG ACATGGCTTTCAACGTGTTCTTCCTTCTCTACTTCGGCTTGCGGTTTATTGCAGCCAACGATAAATTGTG GTTCTGGCTGGAAGTGAACTCTGTAGTGGATTTCTTCACGGTGCCCCCCGTGTTTGTGTCTGTGTACTTA AACAGAAGTTGGCTTGGTTTGAGATTTTTAAGAGCTCTGAGACTGATACAGTTTTCAGAAATTTTGCAGT TTCTGAATATTCTTAAAACAAGTAATTCCATCAAGCTGGTGAATCTGCTCTCCATATTTATCAGCACGTG GCTGACTGCAGCCGGGTTCATCCATTTGGTGGAGAATTCAGGGGACCCATGGGAAAATTTCCAAAACAAC CAGGCTCTCACCTACTGGGAATGTGTCTATTTACTCATGGTCACAATGTCCACCGTTGGTTATGGGGATG TTTATGCAAAAACCACACTTGGGCGCCTCTTCATGGTCTTCTTCATCCTCGGGGGACTGGCCATGTTTGC CAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATACGGGGGCTCCTATAGTGCGGTT AGTGGAAGAAAGCACATTGTGGTCTGCGGACACATCACTCTGGAGAGTGTTTCCAACTTCCTGAAGGACT TTCTGCACAAGGACCGGGATGACGTCAATGTGGAGATCGTTTTTCTTCACAACATCTCCCCCAACCTGGA GCTTGAAGCTCTGTTCAAACGACATTTTACTCAGGTGGAATTTTATCAGGGTTCCGTCCTCAATCCACAT GATCTTGCAAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTTGCCAACAAGTACTGCGCTGACC CGGATGCGGAGGATGCCTCGAATATCATGAGAGTAATCTCCATAAAGAACTACCATCCGAAGATAAGAAT CATCACTCAAATGCTGCAGTATCACAACAAGGCCCATCTGCTAAACATCCCGAGCTGGAATTGGAAAGAA GGTGATGACGCAATCTGCCTCGCAGAGTTGAAGTTGGGCTTCATAGCCCAGAGCTGCCTGGCTCAAGGCC TCTCCACCATGCTTGCCAACCTCTTCTCCATGAGGTCATTCATAAAGATTGAGGAAGACACATGGCAGAA ATACTACTTGGAAGGAGTCTCAAATGAAATGTACACAGAATATCTCTCCAGTGCCTTCGTGGGTCTGTCC TTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTCAAGCTCCTAATGATAGCCATTGAGTACAAGTCTG CCAACCGAGAGAGCCGAAGCCGAAAGCGTATATTAATTAATCCTGGAAACCATCTTAAGATCCAAGAAGG TACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAGGGCATTTTTTTACTGCAAGGCCTGT CATGATGACATCACAGATCCCAAAAGAATAAAAAAATGTGGCTGCAAACGGCTTGAAGATGAGCAGCCGT CAACACTATCACCAAAAAAAAAGCAACGGAATGGAGGCATGCGGAACTCACCCAACACCTCGCCTAAGCT GATGAGGCATGACCCCTTGTTAATTCCTGGCAATGATCAGATTGACAACATGGACTCCAATGTGAAGAAG TACGACTCTACTGGGATGTTTCACTGGTGTGCACCCAAGGAGATAGAGAAAGTCATCCTGACTCGAAGTG AAGCTGCCATGACCGTCCTGAGTGGCCATGTCGTGGTCTGCATCTTTGGCGACGTCAGCTCAGCCCTGAT CGGCCTCCGGAACCTGGTGATGCCGCTCCGTGCCAGCAACTTTCATTACCATGAGCTCAAGCACATTGTG TTTGTGGGCTCTATTGAGTACCTCAAGCGGGAATGGGAGACGCTTCATAACTTCCCCAAAGTGTCCATAT TGCCTGGTACGCCATTAAGTCGGGCTGATTTAAGGGCTGTCAACATCAACCTCTGTGACATGTGCGTTAT CCTGTCAGCCAATCAGAATAATATTGATGATACTTCGCTGCAGGACAAGGAATGCATCTTGGCGTCACTC AACATCAAATCTATGCAGTTTGATGACAGCATCGGAGTCTTGCAGGCTAATTCCCAAGGGTTCACACCTC CAGGAATGGATAGATCCTCTCCAGATAACAGCCCAGTGCACGGGATGTTACGTCAACCATCCATCACAAC TGGGGTCAACATCCCCATCATCACTGAACTAGTGAACGATACTAATGTTCAGTTTTTGGACCAAGACGAT GATGATGACCCTGATACAGAACTGTACCTCACGCAGCCCTTTGCCTGTGGGACAGCATTTGCCGTCAGTG TCCTGGACTCACTCATGAGCGCGACGTACTTCAATGACAATATCCTCACCCTGATACGGACCCTGGTGAC CGGAGGAGCCACGCCGGAGCTGGAGGCTCTGATTGCTGAGGAAAACGCCCTTAGAGGTGGCTACAGCACC CCGCAGACACTGGCCAATAGGGACCGCTGCCGCGTGGCCCAGTTAGCTCTGCTCGATGGGCCATTTGCGG ACTTAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAAAGCTCTGAAAACATATAATATGCTTTGTTT TGGAATTTACCGGCTGAGAGATGCTCACCTCAGCACCCCCAGTCAGTGCACAAAGAGGTATGTCATCACC AACCCGCCCTATGAGTTTGAGCTCGTGCCGACGGACCTGATCTTCTGCTTAATGCAGTTTGACCACAATG CCGGCCAGTCCCGGGCCAGCCTGTCCCATTCCTCCCACTCGTCGCAGTCCTCCAGCAAGAAGAGCTCCTC TGTTCACTCCATCCCATCCACAGCAAACCGACAGAACCGGCCCAAGTCCAGGGAGTCCCGGGACAAACAG AACAGAAAAGAAATGGTTTACAGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235178 representing NM_001271518 Red=Cloning site Green=Tags(s) MANGGGGGGGSSGGGGGGGGSSLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSSVHEPKMDALI IPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKVVLVFALSIGALVI YFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYL NRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNN QALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAV SGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPH DLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKE GDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLS FPTVCELCFVKLKLLMIAIEYKSANRESRSRKRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKAC HDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKK YDSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIV FVGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASL NIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDD DDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYST PQTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVIT NPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRQNRPKSRESRDKQ NRKEMVYR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271518
ORF Size	3384 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271518.2
RefSeq Size	11832 bp
RefSeq ORF	3387 bp
Locus ID	3778
UniProt ID	Q12791
Protein Families	Druggable Genome, Ion Channels: Potassium, Transmembrane
Protein Pathways	Vascular smooth muscle contraction
MW	126.3 kDa
Gene Summary	MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

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