TAOK2 (NM_001252043) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MAP3K17; PSK; PSK1; PSK1-BETA; TAO1; TAO2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235175 representing NM_001252043 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCTGGGGGCCGGGCCGGGAGCCTGAAGGACCCAGATGTGGCTGAGCTCTTCTTCAAGGATGACC CAGAAAAGCTCTTCTCTGACCTCCGGGAAATTGGCCATGGCAGCTTTGGAGCCGTATACTTTGCCCGGGA TGTCCGGAATAGTGAGGTGGTGGCCATCAAGAAGATGTCCTACAGTGGGAAGCAGTCCAATGAGAAATGG CAAGACATCATCAAGGAGGTGCGGTTCTTACAGAAGCTCCGGCATCCCAACACCATTCAGTACCGGGGCT GTTACCTGAGGGAGCACACGGCTTGGCTGGTAATGGAGTATTGCCTGGGCTCAGCTTCTGACCTTCTAGA AGTGCACAAGAAACCCCTTCAGGAGGTAGAGATCGCAGCTGTGACCCACGGGGCGCTTCAGGGCCTGGCA TATCTGCACTCCCACAACATGATCCATAGGGATGTGAAGGCTGGAAACATCCTGCTGTCAGAGCCAGGGT TAGTGAAGCTAGGGGACTTTGGTTCTGCGTCCATCATGGCACCTGCCAACTCCTTCGTGGGCACCCCATA CTGGATGGCACCCGAGGTGATCCTGGCCATGGATGAGGGGCAGTACGATGGCAAAGTGGACGTCTGGTCC TTGGGGATAACCTGCATCGAGCTGGCTGAACGGAAACCACCGCTCTTTAACATGAATGCGATGAGTGCCT TATACCACATTGCACAGAACGAATCCCCCGTGCTCCAGTCAGGACACTGGTCTGAGTACTTCCGGAATTT TGTCGACTCCTGTCTTCAGAAAATCCCTCAAGACAGACCAACCTCAGAGGTTCTCCTGAAGCACCGCTTT GTGCTCCGGGAGCGGCCACCCACAGTCATCATGGACCTGATCCAGAGGACCAAGGATGCCGTGCGGGAGC TGGACAACCTGCAGTACCGCAAGATGAAGAAGATCCTGTTCCAAGAGGCACCCAACGGCCCTGGTGCCGA GGCCCCAGAGGAGGAAGAGGAGGCCGAGCCCTACATGCACCGGGCCGGGACTCTGACCAGCCTCGAGAGT AGCCACTCAGTGCCCAGCATGTCCATCAGCGCCTCCAGCCAGAGCAGCTCCGTCAACAGCCTAGCAGATG CCTCAGACAACGAGGAAGAGGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAAGGCCCTGAAGCCCG GGAGATGGCCATGATGCAGGAGGGGGAGCACACAGTCACCTCTCACAGCTCCATTATCCACCGGCTGCCG GGCTCTGACAACCTATATGATGACCCCTACCAGCCAGAGATAACCCCCAGCCCTCTCCAGCCGCCTGCAG CCCCAGCTCCCACTTCCACCACCTCTTCCGCCCGCCGCCGGGCCTACTGCCGTAACCGAGACCACTTTGC CACCATCCGAACCGCCTCCCTGGTCAGCCGTCAGATCCAGGAGCATGAGCAGGACTCTGCGCTGCGGGAG CAGCTGAGCGGCTATAAGCGGATGCGACGACAGCACCAGAAGCAGCTGCTGGCCCTGGAGTCACGGCTGA GGGGTGAACGGGAGGAGCACAGTGCACGGCTGCAGCGGGAGCTTGAGGCGCAGCGGGCTGGCTTTGGGGC AGAGGCAGAAAAGCTGGCCCGGCGGCACCAGGCCATAGGTGAGAAGGAGGCACGAGCTGCCCAGGCCGAG GAGCGGAAGTTCCAGCAGCACATCCTTGGGCAGCAGAAGAAGGAGCTGGCTGCCCTGCTGGAGGCACAGA AGCGGACCTACAAACTTCGCAAGGAACAGCTGAAGGAGGAGCTCCAGGAGAACCCCAGCACTCCCAAGCG GGAGAAGGCCGAGTGGCTGCTGCGGCAGAAGGAGCAGCTCCAGCAGTGCCAGGCGGAGGAGGAAGCAGGG CTGCTGCGGCGGCAGCGCCAGTACTTTGAGCTGCAGTGTCGCCAGTACAAGCGCAAGATGTTGCTGGCTC GGCACAGCCTGGACCAGGACCTGCTGCGGGAGGACCTGAACAAGAAGCAGACCCAGAAGGACTTGGAGTG TGCACTGCTGCTTCGGCAGCACGAGGCCACGCGGGAGCTGGAGCTGCGGCAGCTCCAGGCCGTGCAGCGC ACGCGGGCTGAGCTCACCCGCCTGCAGCACCAGACGGAGCTGGGCAACCAGCTGGAGTACAACAAGCGGC GTGAGCAAGAGTTGCGGCAGAAGCATGCGGCCCAGGTTCGCCAGCAGCCCAAGAGCCTCAAAGAGAGGAG CATTGTTGGCCAGGAGGAGGCTGGGACATGGAGCTTGTGGGGGAAGGAGGATGAGAGTCTTCTGGATGAG GAGTTTGAGCTTGGCTGGGTCCAGGGCCCAGCACTGACTCCCGTCCCTGAGGAGGAGGAAGAAGAGGAAG AGGGGGCTCCGATTGGGACCCCTAGGGATCCTGGAGATGGTTGTCCTTCCCCCGACATCCCTCCTGAACC CCCTCCAACACACCTGAGGCCCTGCCCTGCCAGCCAGCTCCCTGGACTCCTGTCCCATGGCCTCCTGGCC GGCCTCTCCTTTGCAGTGGGGTCCTCCTCTGGCCTCCTGCCCCTCCTGCTGCTGCTGCTGCTTCCATTGC TGGCAGCCCAGGGTGGGGGTGGCCTGCAGGCAGCGCTGCTGGCCCTTGAGGTGGGGCTGGTGGGTCTGGG GGCCTCCTACCTGCTCCTTTGTACAGCCCTGCACCTGCCCTCCAGTCTTTTCCTACTCCTGGCCCAGGGT ACCGCACTGGGGGCCGTCCTGGGCCTGAGCTGGCGCCGAGGCCTCATGGGTGTTCCCCTGGGCCTTGGAG CTGCCTGGCTCTTAGCTTGGCCAGGCCTAGCTCTACCTCTGGTGGCTATGGCAGCGGGGGGCAGATGGGT GCGGCAGCAGGGCCCCCGGGTGCGCCGGGGCATATCTCGACTCTGGTTGCGGGTTCTGCTGCGCCTGTCA CCCATGGCCTTCCGGGCCCTGCAGGGCTGTGGGGCTGTGGGGGACCGGGGTCTGTTTGCACTGTACCCCA AAACCAACAAGGATGGCTTCCGCAGCCGCCTGCCCGTCCCTGGGCCCCGGCGGCGTAATCCCCGCACCAC CCAACACCCATTAGCTCTGTTGGCAAGGGTCTGGGTCCTGTGCAAGGGCTGGAACTGGCGTCTGGCACGG GCCAGCCAGGGTTTAGCATCCCACTTGCCCCCGTGGGCCATCCACACACTGGCCAGCTGGGGCCTGCTTC GGGGTGAACGGCCCACCCGAATCCCCCGGCTACTACCACGCAGCCAGCGCCAGCTAGGGCCCCCTGCCTC CCGCCAGCCACTGCCAGGGACTCTAGCCGGGCGGAGGTCACGCACCCGCCAGTCCCGGGCCCTGCCCCCC TGGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235175 representing NM_001252043 Red=Cloning site Green=Tags(s) MPAGGRAGSLKDPDVAELFFKDDPEKLFSDLREIGHGSFGAVYFARDVRNSEVVAIKKMSYSGKQSNEKW QDIIKEVRFLQKLRHPNTIQYRGCYLREHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAVTHGALQGLA YLHSHNMIHRDVKAGNILLSEPGLVKLGDFGSASIMAPANSFVGTPYWMAPEVILAMDEGQYDGKVDVWS LGITCIELAERKPPLFNMNAMSALYHIAQNESPVLQSGHWSEYFRNFVDSCLQKIPQDRPTSEVLLKHRF VLRERPPTVIMDLIQRTKDAVRELDNLQYRKMKKILFQEAPNGPGAEAPEEEEEAEPYMHRAGTLTSLES SHSVPSMSISASSQSSSVNSLADASDNEEEEEEEEEEEEEEEGPEAREMAMMQEGEHTVTSHSSIIHRLP GSDNLYDDPYQPEITPSPLQPPAAPAPTSTTSSARRRAYCRNRDHFATIRTASLVSRQIQEHEQDSALRE QLSGYKRMRRQHQKQLLALESRLRGEREEHSARLQRELEAQRAGFGAEAEKLARRHQAIGEKEARAAQAE ERKFQQHILGQQKKELAALLEAQKRTYKLRKEQLKEELQENPSTPKREKAEWLLRQKEQLQQCQAEEEAG LLRRQRQYFELQCRQYKRKMLLARHSLDQDLLREDLNKKQTQKDLECALLLRQHEATRELELRQLQAVQR TRAELTRLQHQTELGNQLEYNKRREQELRQKHAAQVRQQPKSLKERSIVGQEEAGTWSLWGKEDESLLDE EFELGWVQGPALTPVPEEEEEEEEGAPIGTPRDPGDGCPSPDIPPEPPPTHLRPCPASQLPGLLSHGLLA GLSFAVGSSSGLLPLLLLLLLPLLAAQGGGGLQAALLALEVGLVGLGASYLLLCTALHLPSSLFLLLAQG TALGAVLGLSWRRGLMGVPLGLGAAWLLAWPGLALPLVAMAAGGRWVRQQGPRVRRGISRLWLRVLLRLS PMAFRALQGCGAVGDRGLFALYPKTNKDGFRSRLPVPGPRRRNPRTTQHPLALLARVWVLCKGWNWRLAR ASQGLASHLPPWAIHTLASWGLLRGERPTRIPRLLPRSQRQLGPPASRQPLPGTLAGRRSRTRQSRALPP WR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252043
ORF Size	3366 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252043.2
RefSeq Size	4621 bp
RefSeq ORF	3369 bp
Locus ID	9344
UniProt ID	Q9UL54
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways	MAPK signaling pathway
MW	126.7 kDa
Gene Summary	This gene encodes a serine/threonine protein kinase that is involved in many different processes, including, cell signaling, microtubule organization and stability, and apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

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