ATP1A3 (NM_001256213) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AHC2; ATP1A1; CAPOS; DYT12; RDP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235125 representing NM_001256213 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGGCTGGGAGGAGGAGAGGAACAGGAGAGCCACGGACAAGAAAGATGACAAGGACTCACCCAAGA AGAACAAGGGCAAGGAGCGCCGGGACCTGGATGACCTCAAGAAGGAGGTGGCTATGACAGAGCACAAGAT GTCAGTGGAAGAGGTCTGCCGGAAATACAACACAGACTGTGTGCAGGGTTTGACCCACAGCAAAGCCCAG GAGATCCTGGCCCGGGATGGGCCTAACGCACTCACGCCACCGCCTACCACCCCAGAGTGGGTCAAGTTTT GCCGGCAGCTCTTCGGGGGCTTCTCCATCCTGCTGTGGATCGGGGCTATCCTCTGCTTCCTGGCCTACGG TATCCAGGCGGGCACCGAGGACGACCCCTCTGGTGACAACCTGTACCTGGGCATCGTGCTGGCGGCCGTG GTGATCATCACTGGCTGCTTCTCCTACTACCAGGAGGCCAAGAGCTCCAAGATCATGGAGTCCTTCAAGA ACATGGTGCCCCAGCAAGCCCTGGTGATCCGGGAAGGTGAGAAGATGCAGGTGAACGCTGAGGAGGTGGT GGTCGGGGACCTGGTGGAGATCAAGGGTGGAGACCGAGTGCCAGCTGACCTGCGGATCATCTCAGCCCAC GGCTGCAAGGTGGACAACTCCTCCCTGACTGGCGAATCCGAGCCCCAGACTCGCTCTCCCGACTGCACTC ACGACAACCCCTTGGAGACTCGGAACATCACCTTCTTTTCCACCAACTGTGTGGAAGGCACGGCTCGGGG CGTGGTGGTGGCCACGGGCGACCGCACTGTCATGGGCCGTATCGCCACCCTGGCATCAGGGCTGGAGGTG GGCAAGACGCCCATCGCCATCGAGATTGAGCACTTCATCCAGCTCATCACCGGCGTGGCTGTCTTCCTGG GTGTCTCCTTCTTCATCCTCTCCCTCATTCTCGGATACACCTGGCTTGAGGCTGTCATCTTCCTCATCGG CATCATCGTGGCCAATGTCCCAGAGGGTCTGCTGGCCACTGTCACTGTGTGTCTGACGCTGACCGCCAAG CGCATGGCCCGGAAGAACTGCCTGGTGAAGAACCTGGAGGCTGTAGAAACCCTGGGCTCCACGTCCACCA TCTGCTCAGATAAGACAGGGACCCTCACTCAGAACCGCATGACAGTCGCCCACATGTGGTTTGACAACCA GATCCACGAGGCTGACACCACTGAGGACCAGTCAGGGACCTCATTTGACAAGAGTTCGCACACCTGGGTG GCCCTGTCTCACATCGCTGGGCTCTGCAATCGCGCTGTCTTCAAGGGTGGTCAGGACAACATCCCTGTGC TCAAGAGGGATGTGGCTGGGGATGCGTCTGAGTCTGCCCTGCTCAAGTGCATCGAGCTGTCCTCTGGCTC CGTGAAGCTGATGCGTGAACGCAACAAGAAAGTGGCTGAGATTCCCTTCAATTCCACCAACAAATACCAG CTCTCCATCCATGAGACCGAGGACCCCAACGACAACCGATACCTGCTGGTGATGAAGGGTGCCCCCGAGC GCATCCTGGACCGCTGCTCCACCATCCTGCTACAGGGCAAGGAGCAGCCTCTGGACGAGGAAATGAAGGA GGCCTTCCAGAATGCCTACCTTGAGCTCGGTGGCCTGGGCGAGCGCGTGCTTGGTTTCTGCCATTATTAC CTGCCCGAGGAGCAGTTCCCCAAGGGCTTTGCCTTCGACTGTGATGACGTGAACTTCACCACGGACAACC TCTGCTTTGTGGGCCTCATGTCCATGATCGACCCACCCCGGGCAGCCGTCCCTGACGCGGTGGGCAAGTG TCGCAGCGCAGGCATCAAGGTCATCATGGTCACCGGCGATCACCCCATCACGGCCAAGGCCATTGCCAAG GGTGTGGGCATCATCTCTGAGGGCAACGAGACTGTGGAGGACATCGCCGCCCGGCTCAACATTCCCGTCA GCCAGGTTAACCCCCGGGATGCCAAGGCCTGCGTGATCCACGGCACCGACCTCAAGGACTTCACCTCCGA GCAAATCGACGAGATCCTGCAGAATCACACCGAGATCGTCTTCGCCCGCACATCCCCCCAGCAGAAGCTC ATCATTGTGGAGGGCTGTCAGAGACAGGGTGCAATTGTGGCTGTGACCGGGGATGGTGTGAACGACTCCC CCGCTCTGAAGAAGGCCGACATTGGGGTGGCCATGGGCATCGCTGGCTCTGACGTCTCCAAGCAGGCAGC TGACATGATCCTGCTGGACGACAACTTTGCCTCCATCGTCACAGGGGTGGAGGAGGGCCGCCTGATCTTC GACAACCTAAAGAAGTCCATTGCCTACACCCTGACCAGCAATATCCCGGAGATCACGCCCTTCCTGCTGT TCATCATGGCCAACATCCCGCTGCCCCTGGGCACCATCACCATCCTCTGCATCGATCTGGGCACTGACAT GGTCCCTGCCATCTCACTGGCGTACGAGGCTGCCGAAAGCGACATCATGAAGAGACAGCCCAGGAACCCG CGGACGGACAAATTGGTCAATGAGAGACTCATCAGCATGGCCTACGGGCAGATTGGAATGATCCAGGCTC TCGGTGGCTTCTTCTCTTACTTTGTGATCCTGGCAGAAAATGGCTTCTTGCCCGGCAACCTGGTGGGCAT CCGGCTGAACTGGGATGACCGCACCGTCAATGACCTGGAAGACAGTTACGGGCAGCAGTGGACATACGAG CAGAGGAAGGTGGTGGAGTTCACCTGCCACACGGCCTTCTTTGTGAGCATCGTTGTCGTCCAGTGGGCCG ATCTGATCATCTGCAAGACCCGGAGGAACTCGGTCTTCCAGCAGGGCATGAAGAACAAGATCCTGATCTT CGGGCTGTTTGAGGAGACGGCCCTGGCTGCCTTCCTGTCCTACTGCCCCGGCATGGACGTGGCCCTGCGC ATGTACCCTCTCAAGCCCAGCTGGTGGTTCTGTGCCTTCCCCTACAGTTTCCTCATCTTCGTCTACGACG AAATCCGCAAACTCATCCTGCGCAGGAACCCAGGGGGTTGGGTGGAGAAGGAAACCTACTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235125 representing NM_001256213 Red=Cloning site Green=Tags(s) MGGWEEERNRRATDKKDDKDSPKKNKGKERRDLDDLKKEVAMTEHKMSVEEVCRKYNTDCVQGLTHSKAQ EILARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGAILCFLAYGIQAGTEDDPSGDNLYLGIVLAAV VIITGCFSYYQEAKSSKIMESFKNMVPQQALVIREGEKMQVNAEEVVVGDLVEIKGGDRVPADLRIISAH GCKVDNSSLTGESEPQTRSPDCTHDNPLETRNITFFSTNCVEGTARGVVVATGDRTVMGRIATLASGLEV GKTPIAIEIEHFIQLITGVAVFLGVSFFILSLILGYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAK RMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGTSFDKSSHTWV ALSHIAGLCNRAVFKGGQDNIPVLKRDVAGDASESALLKCIELSSGSVKLMRERNKKVAEIPFNSTNKYQ LSIHETEDPNDNRYLLVMKGAPERILDRCSTILLQGKEQPLDEEMKEAFQNAYLELGGLGERVLGFCHYY LPEEQFPKGFAFDCDDVNFTTDNLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAK GVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVIHGTDLKDFTSEQIDEILQNHTEIVFARTSPQQKL IIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIF DNLKKSIAYTLTSNIPEITPFLLFIMANIPLPLGTITILCIDLGTDMVPAISLAYEAAESDIMKRQPRNP RTDKLVNERLISMAYGQIGMIQALGGFFSYFVILAENGFLPGNLVGIRLNWDDRTVNDLEDSYGQQWTYE QRKVVEFTCHTAFFVSIVVVQWADLIICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMDVALR MYPLKPSWWFCAFPYSFLIFVYDEIRKLILRRNPGGWVEKETYY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256213
ORF Size	3072 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256213.1, NP_001243142.1
RefSeq Size	3504 bp
RefSeq ORF	3075 bp
Locus ID	478
UniProt ID	P13637
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cardiac muscle contraction
MW	113.6 kDa
Gene Summary	The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

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