EFTUD2 (NM_001258354) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MFDGA; MFDM; SNRNP116; Snrp116; Snu114; U5-116KD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235082 representing NM_001258354 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATACCGACTTATATGATGAGTTTGGGAATTATATTGGACCAGAGCTTGATTCTGATGAAGATGATG ATGAATTGGGTAGAGAGACCAAAGATCTTGATGAGATGGATGATGATGACGACGACGATGACGTAGGAGA TCATGACGATGACCACCCTGGGATGGAGGTGGTGCTGCATGAGGACAAGAAGTACTACCCAACAGCCGAG GAGGTGTATGGTCCTGAGGTGGAGACCATAGTTCAAGAGGAAGACACTCAGCCTCTCACAGAACCCATTA TTAAGCCAGTGAAAACCAAGAAATTCACTCTGATGGAGCAGACATTACCTGTTACGGTGTATGAGATGGA TTTCTTGGCGGATCTGATGGATAACTCAGAGCTCATCAGAAATGTGACCCTTTGTGGACATCTCCACCAT GGCAAGACTCACCCGGAAATCAGAAAGCGCTATGACCAAGATCTGTGCTATACTGACATCCTCTTCACAG AGCAAGAGAGAGGTGTAGGCATCAAAAGCACTCCTGTGACAGTGGTCTTGCCAGACACCAAAGGAAAATC TTATCTCTTCAATATCATGGACACTCCAGGACATGTGAATTTCTCTGATGAGGTCACAGCTGGCTTGCGC ATCTCAGATGGAGTGGTCCTTTTCATTGATGCTGCTGAGGGGGTGATGCTGAACACAGAGCGGCTGATCA AGCATGCGGTGCAGGAGAGGCTGGCAGTCACTGTGTGCATCAACAAGATTGACCGGCTGATCCTGGAGCT GAAGCTGCCTCCAACTGATGCTTATTACAAGCTGCGCCACATTGTGGATGAGGTCAATGGATTAATAAGC ATGTATTCCACTGATGAGAACCTGATCCTTTCCCCACTCCTGGGTAACGTCTGCTTCTCCAGCTCCCAGT ACAGCATCTGCTTCACGCTGGGCTCCTTTGCCAAGATCTATGCCGACACCTTTGGTGACATTAATTACCA AGAATTTGCTAAAAGACTCTGGGGTGACATCTACTTCAACCCTAAGACGCGAAAGTTCACCAAAAAGGCC CCAACTAGCAGCTCCCAGAGAAGTTTCGTGGAGTTTATCTTGGAGCCTCTTTATAAGATCCTCGCCCAGG TTGTAGGTGACGTGGACACCAGCCTCCCACGGACCCTAGACGAGCTTGGCATCCACCTGACGAAGGAGGA GCTGAAGCTGAACATCCGCCCCTTGCTCAGGCTGGTCTGCAAAAAGTTCTTTGGCGAGTTCACAGGCTTT GTGGACATGTGTGTGCAGCATATCCCTTCTCCAAAGGTGGGCGCCAAGCCCAAGATTGAGCACACCTACA CCGGTGGTGTGGACTCCGACCTCGGCGAGGCTATGAGTGACTGTGACCCTGATGGCCCCCTGATGTGCCA CACTACTAAGATGTACAGCACAGATGATGGAGTCCAGTTTCACGCCTTTGGCCGGGTGCTGAGTGGCACC ATTCATGCTGGGCAGCCTGTGAAGGTACTGGGGGAGAACTACACCCTGGAGGATGAGGAAGACTCCCAGA TATGCACCGTGGGCCGCCTTTGGATCTCTGTGGCCAGGTACCACATCGAGGTGAACCGTGTTCCTGCTGG CAACTGGGTTCTGATTGAAGGTGTTGATCAACCAATTGTGAAGACAGCAACCATAACCGAACCCCGAGGC AATGAGGAGGCTCAGATTTTCCGACCCTTGAAGTTCAATACCACATCTGTTATCAAGATTGCTGTGGAGC CAGTCAACCCCTCAGAGCTGCCCAAGATGCTTGATGGCCTGCGCAAGGTCAACAAGAGCTATCCATCCCT CACCACCAAGGTGGAGGAGTCTGGCGAGCATGTGATCCTGGGCACTGGGGAGCTCTACCTGGACTGTGTG ATGCATGATTTGCGGAAGATGTACTCAGAGATAGACATCAAGGTGGCTGACCCAGTTGTCACGTTTTGTG AGACGGTGGTGGAAACATCCTCCCTCAAGTGCTTTGCTGAAACGCCTAATAAGAAGAACAAGATCACCAT GATTGCTGAGCCTCTTGAGAAGGGCCTGGCAGAGGACATAGAGAATGAGGTGGTCCAGATTACGTGGAAC AGGAAGAAGCTGGGAGAGTTCTTCCAGACCAAGTACGATTGGGATCTGCTGGCTGCCCGTTCCATCTGGG CTTTTGGCCCTGATGCGACTGGCCCCAACATTCTGGTGGATGATACTCTGCCCTCTGAGGTGGACAAGGC TCTTCTTGGTTCAGTGAAGGACAGCATCGTTCAAGGTTTCCAGTGGGGAACCAGGGAGGGCCCCCTCTGT GATGAATTGATTCGGAATGTCAAGTTTAAGATCCTGGATGCGGTGGTTGCCCAGGAGCCCCTGCACCGGG GCGGGGGCCAGATCATCCCCACAGCCAGGAGAGTCGTCTACTCTGCCTTCCTCATGGCTACTCCTCGTCT GATGGAGCCTTACTACTTTGTAGAGGTCCAGGCCCCTGCAGATTGCGTCTCTGCAGTTTATACCGTCCTG GCCAGGCGCAGGGGGCACGTGACTCAGGATGCACCCATCCCAGGCTCCCCTCTGTACACCATCAAAGCTT TTATCCCGGCCATCGACTCTTTTGGCTTTGAGACTGATCTCCGGACTCACACCCAGGGACAAGCCTTTTC TCTGTCTGTCTTCCACCACTGGCAGATTGTGCCTGGTGATCCCCTGGACAAGAGCATTGTCATCCGCCCC TTGGAGCCACAGCCAGCTCCTCACCTGGCCCGGGAATTCATGATCAAAACCCGCCGTAGGAAGGGCCTCA GTGAAGATGTGAGCATCAGCAAATTCTTCGATGATCCTATGTTGCTGGAACTTGCCAAACAGGATGTTGT GCTCAATTACCCCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235082 representing NM_001258354 Red=Cloning site Green=Tags(s) MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAE EVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHH GKTHPEIRKRYDQDLCYTDILFTEQERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLR ISDGVVLFIDAAEGVMLNTERLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLIS MYSTDENLILSPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVCKKFFGEFTGF VDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTKMYSTDDGVQFHAFGRVLSGT IHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIEVNRVPAGNWVLIEGVDQPIVKTATITEPRG NEEAQIFRPLKFNTTSVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCV MHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWN RKKLGEFFQTKYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVL ARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSVFHHWQIVPGDPLDKSIVIRP LEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLELAKQDVVLNYPM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001258354
ORF Size	2886 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258354.1, NP_001245283.1
RefSeq Size	4137 bp
RefSeq ORF	2889 bp
Locus ID	9343
UniProt ID	Q15029
Protein Pathways	Spliceosome
MW	108.7 kDa
Gene Summary	This gene encodes a GTPase which is a component of the spliceosome complex which processes precursor mRNAs to produce mature mRNAs. Mutations in this gene are associated with mandibulofacial dysostosis with microcephaly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

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