ADCY5 (NM_001199642) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AC5; FDFM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235022 representing NM_001199642 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTCTCAGAAAGAGGGCTGTTGCAGCAGAGGTGACCTGTCCATTCAGACAGGTCCTGGAGGAGAAT GGGCCCCACGGAGGCTTGTCTCCAATGTTCTCATTTTCTCCTGCACCAACATCGTGGGTGTCTGCACCCA CTATCCGGCTGAGGTCTCCCAGAGACAGGCTTTCCAGGAGACCCGAGAGTGCATCCAGGCGCGGCTCCAC TCGCAGCGGGAGAACCAGCAGCAGGAACGGCTCCTGCTGTCTGTCCTTCCCCGTCATGTTGCCATGGAGA TGAAAGCAGACATCAACGCCAAGCAGGAGGATATGATGTTCCATAAGATTTACATCCAGAAACATGACAA CGTGAGCATCCTGTTTGCTGACATCGAGGGCTTCACCAGCCTGGCGTCCCAGTGCACTGCACAGGAACTG GTCATGACCCTCAACGAGCTCTTCGCCCGCTTTGACAAGCTGGCCGCAGAGAATCACTGTTTACGTATTA AGATCCTTGGGGATTGTTATTACTGCGTCTCGGGGCTGCCTGAAGCAAGGGCTGACCACGCCCACTGCTG TGTGGAGATGGGCATGGACATGATCGAGGCCATCTCGTTGGTCCGGGAGGTGACAGGGGTGAACGTGAAC ATGCGTGTGGGAATTCACAGCGGGCGAGTACACTGCGGTGTCCTTGGTCTCAGGAAGTGGCAGTTCGACG TCTGGTCTAACGATGTCACGCTAGCCAACCACATGGAGGCTGGCGGCAAGGCAGGACGCATCCACATCAC CAAGGCTACACTCAACTACCTGAATGGGGACTACGAGGTGGAGCCAGGCTGTGGGGGCGAGCGCAACGCC TACCTCAAGGAGCACAGTATCGAGACCTTCCTCATCCTGCGCTGCACCCAGAAGCGGAAAGAAGAGAAGG CCATGATCGCCAAGATGAACCGCCAGAGAACCAACTCCATCGGGCACAACCCACCACACTGGGGGGCTGA GCGCCCCTTCTACAACCACCTGGGTGGCAACCAGGTGTCCAAGGAGATGAAGCGGATGGGCTTTGAAGAC CCCAAGGACAAGAACGCCCAGGAGAGTGCGAACCCTGAGGATGAAGTGGATGAGTTTCTGGGCCGTGCCA TTGACGCCAGGAGCATTGATAGGCTTCGGTCTGAGCACGTCCGCAAGTTCCTCCTGACCTTCAGGGAGCC TGACTTAGAGAAGAAGTACTCCAAGCAGGTAGACGACCGATTTGGTGCCTATGTGGCGTGTGCCTCGCTC GTCTTCCTCTTCATCTGCTTTGTCCAGATCACCATCGTGCCCCACTCCATATTCATGCTCAGCTTCTACC TGACCTGTTCCCTGCTGCTGACCTTGGTGGTGTTTGTGTCTGTGATCTACTCCTGCGTAAAGCTCTTCCC CTCCCCACTGCAGACCCTCTCCAGGAAGATCGTGCGGTCCAAGATGAACAGCACCCTGGTTGGGGTGTTC ACCATCACCCTGGTGTTCCTGGCGGCTTTTGTCAACATGTTCACGTGCAACTCCAGGGACCTGCTGGGCT GCTTGGCACAGGAGCACAACATCAGCGCGAGCCAGGTCAACGCGTGTCACGTGGCGGAGTCGGCCGTCAA CTACAGCCTGGGCGATGAGCAGGGCTTCTGTGGCAGCCCCTGGCCCAACTGCAACTTCCCCGAGTACTTC ACCTACAGCGTGCTGCTCAGCCTGCTGGCCTGCTCCGTGTTCCTGCAGATCAGCTGCATCGGGAAGCTGG TGCTCATGCTGGCCATCGAGCTCATCTACGTGCTCATCGTGGAGGTGCCAGGTGTCACGCTCTTCGACAA CGCCGACCTGCTGGTCACCGCCAACGCCATAGACTTCTTCAACAACGGGACCTCCCAGTGCCCTGAGCAT GCAACCAAGGTGGCATTGAAGGTGGTGACGCCCATCATCATCTCAGTCTTTGTGCTGGCCCTGTACCTGC ACGCCCAGCAGGTGGAGTCCACTGCCCGCCTCGACTTCCTCTGGAAACTGCAGGCCACAGAGGAGAAAGA GGAGATGGAGGAGCTGCAGGCCTACAACCGGCGGCTGCTGCACAACATCCTGCCCAAGGACGTGGCCGCT CACTTCCTGGCCCGCGAGCGGCGCAATGATGAGCTCTACTATCAGTCCTGTGAGTGTGTGGCGGTCATGT TCGCCTCCATCGCCAACTTCTCCGAGTTCTACGTTGAGCTGGAGGCCAACAACGAGGGTGTCGAGTGCCT GCGGCTACTCAATGAGATCATCGCTGACTTTGATGAGATCATCAGCGAGGATCGGTTCCGGCAGCTGGAG AAGATCAAGACCATCGGCAGCACCTACATGGCTGCCTCCGGCCTCAACGACTCTACCTACGACAAGGTGG GCAAGACCCACATCAAGGCACTGGCCGACTTTGCCATGAAGCTGATGGACCAGATGAAGTACATCAATGA GCACTCCTTCAACAACTTCCAGATGAAGATCGGGCTCAACATCGGCCCCGTGGTGGCCGGGGTGATAGGG GCACGAAAGCCTCAGTACGACATCTGGGGCAATACCGTGAACGTGGCCAGCCGCATGGACAGCACCGGTG TACCCGACCGCATCCAGGTCACCACAGACATGTACCAGGTGCTGGCTGCCAACACGTACCAGCTGGAGTG CCGGGGCGTGGTCAAGGTCAAGGGCAAAGGCGAGATGATGACCTACTTCCTCAATGGAGGGCCCCCGCTC AGT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235022 representing NM_001199642 Red=Cloning site Green=Tags(s) MKSQKEGCCSRGDLSIQTGPGGEWAPRRLVSNVLIFSCTNIVGVCTHYPAEVSQRQAFQETRECIQARLH SQRENQQQERLLLSVLPRHVAMEMKADINAKQEDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQEL VMTLNELFARFDKLAAENHCLRIKILGDCYYCVSGLPEARADHAHCCVEMGMDMIEAISLVREVTGVNVN MRVGIHSGRVHCGVLGLRKWQFDVWSNDVTLANHMEAGGKAGRIHITKATLNYLNGDYEVEPGCGGERNA YLKEHSIETFLILRCTQKRKEEKAMIAKMNRQRTNSIGHNPPHWGAERPFYNHLGGNQVSKEMKRMGFED PKDKNAQESANPEDEVDEFLGRAIDARSIDRLRSEHVRKFLLTFREPDLEKKYSKQVDDRFGAYVACASL VFLFICFVQITIVPHSIFMLSFYLTCSLLLTLVVFVSVIYSCVKLFPSPLQTLSRKIVRSKMNSTLVGVF TITLVFLAAFVNMFTCNSRDLLGCLAQEHNISASQVNACHVAESAVNYSLGDEQGFCGSPWPNCNFPEYF TYSVLLSLLACSVFLQISCIGKLVLMLAIELIYVLIVEVPGVTLFDNADLLVTANAIDFFNNGTSQCPEH ATKVALKVVTPIIISVFVLALYLHAQQVESTARLDFLWKLQATEEKEEMEELQAYNRRLLHNILPKDVAA HFLARERRNDELYYQSCECVAVMFASIANFSEFYVELEANNEGVECLRLLNEIIADFDEIISEDRFRQLE KIKTIGSTYMAASGLNDSTYDKVGKTHIKALADFAMKLMDQMKYINEHSFNNFQMKIGLNIGPVVAGVIG ARKPQYDIWGNTVNVASRMDSTGVPDRIQVTTDMYQVLAANTYQLECRGVVKVKGKGEMMTYFLNGGPPL S SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199642
ORF Size	2733 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199642.1, NP_001186571.1
RefSeq Size	5123 bp
RefSeq ORF	2736 bp
Locus ID	111
UniProt ID	O95622
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Vascular smooth muscle contraction
MW	103.1 kDa
Gene Summary	This gene encodes a member of the membrane-bound adenylyl cyclase enzymes. Adenylyl cyclases mediate G protein-coupled receptor signaling through the synthesis of the second messenger cAMP. Activity of the encoded protein is stimulated by the Gs alpha subunit of G protein-coupled receptors and is inhibited by protein kinase A, calcium and Gi alpha subunits. Single nucleotide polymorphisms in this gene may be associated with low birth weight and type 2 diabetes. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

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