LONP1 (NM_001276479) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CODASS; hLON; LON; LonHS; LONP; PIM1; PRSS15
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234998 representing NM_001276479 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGAGCACTGGCTACGTGCGACTGTGGGGAGCGGCGCGGTGCTGGGTGCTGCGGCGGCCGATGC TGGCCGCCGCCGGGGGGCGGGTTCCCACTGCAGCAGGAGCGTGGTTGCTCCGAGGCCCGGTCATAACGGC GCTCACGCCCATGACGATCCCCGATGTGTTTCCGCACCTGCCGCTCATCGCCATCACCCGCAACCCGGTG TTCCCGCGCTTTATCAAGATTATCGAGGTTAAAAATAAGAAGTTGGTTGAGCTGCTGAGAAGGAAAGTTC GTCTCGCCCAGCCTTATGTCGGCGTCTTTCTAAAGAGAGATGACAGCAATGAGTCGGATGTGGTCGAGAG CCTGGATGAAATCTACCACACGGGGACGTTTGCCCAGATCCATGAGATGCAGGACCTTGGGGACAAGCTG CGCATGATCGTCATGGGACACAGAAGAGTCCATATCAGCAGACAGCTGGAGGTGGAGCCCGAGGAGCCGG AGGCGGAGAACAAGCACAAGCCCCGCAGGAAGTCAAAGCGGGGCAAGAAGGAGGCGGAGGACGAGCTGAG CGCCAGGCACCCGGCGGAGCTGGCGATGGAGCCCACCCCTGAGCTCCCGGCTGAGGTGCTCATGGTGGAG GTAGAGAACGTTGTCCACGAGGACTTCCAGGTCACGGAGGAGGTGAAAGCCCTGACTGCAGAGATCGTGA AGACCATCCGGGACATCATTGCCTTGAACCCTCTCTACAGGGAGTCAGTGCTGCAGATGATGCAGGCTGG CCAGCGGGTGGTGGACAACCCCATCTACCTGAGCGACATGGGCGCCGCGCTCACCGGGGCCGAGTCCCAT GAGCTGCAGGACGTCCTGGAAGAGACCAATATTCCTAAGCGGCTGTACAAGGCCCTCTCCCTGCTGAAGA AGGAATTTGAACTGAGCAAGCTGCAGCAGCGCCTGGGGCGGGAGGTGGAGGAGAAGATCAAGCAGACCCA CCGTAAGTACCTGCTGCAGGAGCAGCTAAAGATCATCAAGAAGGAGCTGGGCCTGGAGAAGGACGACAAG GATGCCATCGAGGAGAAGTTCCGGGAGCGCCTGAAGGAGCTCGTGGTCCCCAAGCACGTCATGGATGTTG TGGACGAGGAGCTGAGCAAGCTGGGCCTGCTGGACAACCACTCCTCGGAGTTCAATGTCACCCGCAACTA CCTAGACTGGCTCACGTCCATCCCTTGGGGCAAGTACAGCAACGAGAACCTGGACCTGGCGCGGGCACAG GCAGTGCTGGAGGAAGACCACTACGGCATGGAGGACGTCAAGAAACGCATCCTGGAGTTCATTGCCGTTA GCCAGCTCCGCGGCTCCACCCAGGGCAAGATCCTCTGCTTCTATGGCCCCCCTGGCGTGGGTAAGACCAG CATTGCTCGCTCCATCGCCCGCGCCCTGAACCGAGAGTACTTCCGCTTCAGCGTCGGGGGCATGACTGAC GTGGCTGAGATCAAGGGCCACAGGCGGACCTACGTGGGCGCCATGCCCGGGAAGATCATCCAGTGTTTGA AGAAGACCAAGACGGAGAACCCCCTGATCCTCATCGACGAGGTGGACAAGATCGGCCGAGGCTACCAGGG GGACCCGTCGTCGGCACTGCTGGAGCTGCTGGACCCAGAGCAGAATGCCAACTTCCTGGACCACTACCTG GACGTGCCCGTGGACTTGTCCAAGGTGCTGTTCATCTGCACGGCCAACGTCACGGACACCATCCCCGAGC CGCTGCGAGACCGTATGGAGATGATCAACGTGTCGGGCTACGTGGCCCAGGAGAAGCTGGCCATTGCGGA GCGCTACCTGGTGCCCCAGGCTCGCGCCCTGTGTGGCTTGGATGAGAGCAAGGCCAAGCTGTCATCGGAC GTGCTGACGCTGCTCATCAAGCAGTACTGCCGCGAGAGCGGTGTCCGCAACCTGCAGAAGCAAGTGGAGA AGGTGTTACGGAAATCGGCCTACAAGATTGTCAGCGGCGAGGCCGAGTCCGTGGAGGTGACGCCCGAGAA CCTGCAGGACTTCGTGGGGAAGCCCGTGTTCACCGTGGAGCGCATGTATGACGTGACACCGCCCGGCGTG GTCATGGGGCTGGCCTGGACCGCAATGGGAGGCTCCACGCTGTTTGTGGAGACATCCCTGAGACGGCCAC AGGACAAGGATGCCAAGGGTGACAAGGATGGCAGCCTGGAGGTGACAGGCCAGCTGGGGGAGGTGATGAA GGAGAGCGCCCGCATAGCCTACACCTTCGCCAGAGCCTTCCTCATGCAGCACGCCCCCGCCAATGACTAC CTGGTGACCTCACACATCCACCTGCATGTGCCCGAGGGCGCCACCCCCAAGGACGGCCCAAGCGCAGGCT GCACCATCGTCACGGCCCTGCTGTCCCTGGCCATGGGCAGGCCTGTCCGGCAGAATCTGGCCATGACTGG CGAAGTCTCCCTCACGGGCAAGATCCTGCCTGTTGGTGGCATCAAGGAGAAGACCATTGCGGCCAAGCGC GCAGGGGTGACGTGCATCGTCCTGCCAGCCGAGAACAAGAAGGACTTCTACGACCTGGCAGCCTTCATCA CCGAGGGCCTGGAGGTGCACTTCGTGGAACACTACCGGGAGATCTTCGACATCGCCTTCCCGGACGAGCA GGCAGAGGCGCTGGCCGTGGAACGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234998 representing NM_001276479 Red=Cloning site Green=Tags(s) MAASTGYVRLWGAARCWVLRRPMLAAAGGRVPTAAGAWLLRGPVITALTPMTIPDVFPHLPLIAITRNPV FPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKL RMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVE VENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESH ELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDK DAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNVTRNYLDWLTSIPWGKYSNENLDLARAQ AVLEEDHYGMEDVKKRILEFIAVSQLRGSTQGKILCFYGPPGVGKTSIARSIARALNREYFRFSVGGMTD VAEIKGHRRTYVGAMPGKIIQCLKKTKTENPLILIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYL DVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSD VLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGV VMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDY LVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKR AGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276479
ORF Size	2685 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276479.2
RefSeq Size	2936 bp
RefSeq ORF	2688 bp
Locus ID	9361
UniProt ID	P36776
Protein Families	Druggable Genome, Protease
MW	100.4 kDa
Gene Summary	This gene encodes a mitochondrial matrix protein that belongs to the Lon family of ATP-dependent proteases. This protein mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides in the mitochondrial matrix. It may also have a chaperone function in the assembly of inner membrane protein complexes, and participate in the regulation of mitochondrial gene expression and maintenance of the integrity of the mitochondrial genome. Decreased expression of this gene has been noted in a patient with hereditary spastic paraplegia (PMID:18378094). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]

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