PIWIL3 (NM_001255975) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HIWI3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234982 representing NM_001255975 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGGTAGGGCAAGGACTCGCGCCCGAGGCAGAGCCCGCCGCAGGGAGAGCTACCAACAAGAGGCAC CTGGGGGACCCAGAGCACCTGGATCAGCTACAACCCAGGAGCCCCCTCAGTTGCAGTCGACACCCCGGCC GCTGCAGGAGGAAGTCCCAGTGGTTAGACCTCTGCAGCCAAGAGCAGCAAGAGGAGGAGCAGGAGGAGGA GCACAGTCTCAAGGGGTGAAGGAACCTGGACCTGAGGCTGGGTTGCATACAGCGCCCTTGCAGGAGAGAA GGATTGGTGGAGTTTTTCAAGACCTGGTGGTGAACACCAGGCAAGATATGAAGCATGTTAAAGACTCAAA AACAGGTTCAGAGGGTACAGTGGTACAGCTACTCGCCAACCACTTCCGAGTGATATCTCGTCCTCAGTGG GTTGCATATAAATACAACGTTGACTACAAACCAGACATAGAAGATGGAAATCTCCGTACAATTTTACTTG ATCAACATAGAAGGAAATTTGGAGAGCGCCATATATTTGATGGAAACTCTTTATTATTATCTCGGCCACT AAAAGAGCGGAGAGTGGAATGGTTGAGCACAACCAAAGACAAAAACATCGTGAAGATTACAGTTGAGTTT TCCAAAGAACTCACGCCCACGTCGCCAGATTGCCTACGCTATTACAACATTCTCTTTAGAAGAACTTTCA AGCTGCTGGATTTTGAACAAGTTGGTCGCAACTATTATACCAAAAAGAAGGCCATTCAGTTATACCGTCA TGGTACCAGTTTGGAAATCTGGCTTGGTTATGTTACTTCTGTTCTTCAATACGAAAACAGCATTACCCTC TGTGCCGATGTGAGCCACAAACTGCTCCGAATAGAAACTGCTTATGATTTCATAAAGAGAACATCTGCCC AGGCCCAGACAGGAAACATCCGAGAGGAAGTAACTAATAAATTAATTGGATCAATTGTTCTGACAAAATA CAACAACAAAACCTACAGAGTAGATGATATTGATTGGAAGCAGAATCCTGAAGACACATTTAACAAATCA GATGGCAGCAAAATCACCTATATAGACTACTACAGGCAGCAACATAAAGAAATTGTCACAGTGAAGAAAC AGCCACTTTTGGTCAGCCAGGGCAGATGGAAAAAGGGCCTAACGGGTACACAACGTGAACCTATCCTGCT GATTCCTCAGCTGTGCCACATGACAGGTCTAACAGATGAAATATGTAAAGATTATAGCATTGTGAAAGAA TTGGCTAAACATACAAGATTGAGTCCAAGAAGAAGGCATCATACATTAAAAGAATTCATCAATACTCTAC AAGATAATAAAAAAGTACGAGAGTTACTTCAACTCTGGGATTTGAAATTTGATACCAATTTTTTGTCCGT CCCGGGAAGAGTTTTGAAAAACGCAAACATCGTGCAAGGCAGAAGAATGGTTAAAGCCAATTCACAAGGA GACTGGTCAAGAGAAATAAGAGAATTACCCTTACTTAATGCAATGCCACTACATAGTTGGCTCATACTCT ATAGCAGGAGCAGTCACAGAGAAGCCATGTCCTTAAAGGGTCATCTACAGAGTGTCACAGCCCCCATGGG CATAACTATGAAACCAGCAGAAATGATTGAAGTAGATGGTGATGCTAACTCCTATATAGACACATTACGG AAATATACTAGACCAACACTGCAGATGGTGATTTGTATCCTGCCCAATGATGACAAACGTAGATATGACA GCATAAAAAGATACCTATGTACCAAATGCCCAATTCCAAGCCAGTGTGTGGTGAAAAAGACCTTAGAAAA AGTCCAGGCAAGGACCATCGTCACCAAGATTGCCCAGCAGATGAATTGCAAGATGGGAGGAGCCCTCTGG AAGGTGGAGACAGACGTACAAAGAACAATGTTCGTTGGCATTGATTGTTTCCACGATATCGTAAATCGAC AGAAATCAATAGCAGGATTTGTTGCAAGTACCAATGCTGAATTAACAAAGTGGTACTCTCAATGTGTCAT CCAGAAAACAGGAGAAGAGCTTGTGAAAGAGCTGGAGATCTGCTTGAAAGCTGCCCTGGATGTCTGGTGT AAAAACGAATCATCGATGCCACATTCTGTTATTGTGTATCGGGATGGAGTGGGAGATGGTCAGCTTCAAG CATTGCTTGACCATGAAGCGAAAAAGATGTCGACCTACTTAAAAACCATCTCTCCTAACAATTTCACTCT AGCTTTCATTGTGGTGAAGAAACGAATAAACACTAGATTTTTTCTTAAACATGGAAGCAATTTTCAAAAT CCACCTCCAGGAACAGTTATTGATGTAGAGTTGACTAGGAATGAATGGTATGACTTTTTTATTGTGAGTC AGTCTGTGCAAGATGGGACTGTTACCCCCACTCATTATAACGTCATCTATGACACGATTGGCTTGAGCCC AGATACAGTACAGCGTTTAACATATTGTCTATGCCACATGTATTATAATTTGCCAGGCATCATCCGAGTT CCAGCGCCTTGCCACTATGCCCACAAGCTGGCTTACCTCGTGGGGCAGTCCATTCACCAGGAACCGAATC GTTCCTTGTCAACTCGTCTCTTTTACCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234982 representing NM_001255975 Red=Cloning site Green=Tags(s) MPGRARTRARGRARRRESYQQEAPGGPRAPGSATTQEPPQLQSTPRPLQEEVPVVRPLQPRAARGGAGGG AQSQGVKEPGPEAGLHTAPLQERRIGGVFQDLVVNTRQDMKHVKDSKTGSEGTVVQLLANHFRVISRPQW VAYKYNVDYKPDIEDGNLRTILLDQHRRKFGERHIFDGNSLLLSRPLKERRVEWLSTTKDKNIVKITVEF SKELTPTSPDCLRYYNILFRRTFKLLDFEQVGRNYYTKKKAIQLYRHGTSLEIWLGYVTSVLQYENSITL CADVSHKLLRIETAYDFIKRTSAQAQTGNIREEVTNKLIGSIVLTKYNNKTYRVDDIDWKQNPEDTFNKS DGSKITYIDYYRQQHKEIVTVKKQPLLVSQGRWKKGLTGTQREPILLIPQLCHMTGLTDEICKDYSIVKE LAKHTRLSPRRRHHTLKEFINTLQDNKKVRELLQLWDLKFDTNFLSVPGRVLKNANIVQGRRMVKANSQG DWSREIRELPLLNAMPLHSWLILYSRSSHREAMSLKGHLQSVTAPMGITMKPAEMIEVDGDANSYIDTLR KYTRPTLQMVICILPNDDKRRYDSIKRYLCTKCPIPSQCVVKKTLEKVQARTIVTKIAQQMNCKMGGALW KVETDVQRTMFVGIDCFHDIVNRQKSIAGFVASTNAELTKWYSQCVIQKTGEELVKELEICLKAALDVWC KNESSMPHSVIVYRDGVGDGQLQALLDHEAKKMSTYLKTISPNNFTLAFIVVKKRINTRFFLKHGSNFQN PPPGTVIDVELTRNEWYDFFIVSQSVQDGTVTPTHYNVIYDTIGLSPDTVQRLTYCLCHMYYNLPGIIRV PAPCHYAHKLAYLVGQSIHQEPNRSLSTRLFYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001255975
ORF Size	2619 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001255975.1, NP_001242904.1
RefSeq Size	3481 bp
RefSeq ORF	2622 bp
Locus ID	440822
Protein Pathways	Dorso-ventral axis formation
MW	100.6 kDa
Gene Summary	This gene encodes a member of the PIWI subfamily of Argonaute family proteins. This subfamily of proteins contains a PAZ domain, found in proteins involved in RNA-mediated gene silencing, and a C-terminal Piwi domain. The encoded protein is thought to function in maintenance of germline cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

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