CPT1C (NM_001199753) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CATL1; CPT1-B; CPT1P; CPTI-B; CPTIC; SPG73
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234902 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC RCATGGCTGAAGCGCACCAGGCCGTGGGCTTCCGACCCTCGCTGACCTCGGACGGGGCTGAAGTGGAACT CAGTGCCCCTGTGCTGCAGGAGATCTACCTCTCTGGCCTGCGCTCCTGGAAAAGGCATCTCTCACGTTTC TGGAATGACTTTCTCACCGGTGTGTTTCCTGCCAGCCCCCTCAGTTGGCTTTTCCTCTTCAGTGCCATCC AGCTTGCCTGGTTCCTCCAGCTGGATCCTTCCTTAGGACTGATGGAGAAGATCAAAGAGTTGCTGCCTGA CTGGGGTGGACAACACCACGGGCTCCGGGGGGTCCTGGCAGCCGCGCTGTTTGCCTCGTGTTTGTGGGGA GCCCTGATCTTCACACTGCACGTGGCCCTGAGGCTGCTTCTGTCCTACCACGGCTGGCTTCTTGAGCCCC ACGGAGCCATGTCCTCCCCCACCAAGACCTGGCTGGCCCTGGTCCGCATCTTCTCTGGCCGCCACCCGAT GCTGTTCAGTTACCAGCGCTCCCTGCCACGCCAGCCCGTGCCCTCTGTGCAGGACACCGTGCGCAAGTAC CTGGAGTCGGTCCGGCCCATCCTCTCCGACGAGGACTTCGACTGGACCGCGGTCCTGGCGCAGGAATTCC TGAGGCTGCAGGCGTCGCTGCTGCAGTGGTACCTGCGGCTCAAGTCCTGGTGGGCGTCCAATTATGTCAG TGACTGGTGGGAGGAATTTGTGTACCTGCGCTCCCGAAATCCGCTGATGGTGAACAGCAACTATTACATG ATGGACTTCCTGTATGTCACACCCACGCCTCTGCAGGCAGCTCGCGCTGGGAATGCCGTCCATGCCCTCC TCCTGTACCGCCACCGCCTGAACCGCCAGGAGATACCCCCGACTTTGCTGATGGGAATGCGCCCCTTATG CTCTGCCCAGTACGAGAAGATCTTCAACACCACGCGGATTCCAGGGGTCCAAAAAGACTACATCCGCCAC CTCCATGACAGCCAACACGTGGCTGTCTTCCACCGGGGCCGATTCTTCCGCATGGGGACCCACTCCCGAA ACAGCCTGCTTTCCCCGAGAGCCCTGGAGCAGCAGTTTCAGAGAATCCTGGATGATCCCTCACCGGCCTG CCCCCACGAGGAACATCTGGCAGCTCTGACAGCTGCTCCCAGGGGCACGTGGGCCCAGGTGCGGACATCC CTGAAGACCCAGGCAGCGGAGGCCCTGGAGGCGGTGGAAGGGGCCGCTTTCTTTGTGTCACTGGATGCTG AGCCCGCGGGGCTCACCAGGGAGGACCCGGCAGCGTCGTTGGATGCCTACGCCCATGCTCTGCTGGCCGG CCGGGGCCATGATCGCTGGTTTGACAAATCCTTCACCCTAATCGTCTTCTCTAACGGGAAGCTGGGCCTC AGCGTGGAGCACTCCTGGGCCGACTGCCCCATCTCAGGACACATGTGGGAGTTCACTCTGGCTACAGAAT GCTTTCAGCTGGGCTACTCAACAGACGGCCACTGCAAGGGGCACCCGGACCCCACACTACCCCAGCCCCA GCGGCTGCAATGGGACCTTCCAGACCAGATCCACTCCTCCATCTCTCTAGCCCTGAGGGGAGCCAAGATC TTGTCTGAAAATGTCGACTGCCATGTCGTTCCATTCTCCCTATTTGGCAAGAGCTTCATCCGACGCTGCC ACCTCTCTTCAGACAGCTTCATCCAGATCGCCTTGCAACTGGCCCACTTCCGGGACAGGGGTCAATTCTG CCTGACTTATGAGTCGGCCATGACTCGCTTATTCCTGGAAGGCCGGACGGAGACGGTGCGGTCTTGCACG AGGGAGGCCTGCAACTTTGTCAGGGCCATGGAGGACAAAGAGAAGACGGACCCACAGTGCCTCGCCCTGT TCCGCGTGGCAGTGGACAAGCACCAGGCTCTGCTGAAGGCAGCCATGAGCGGGCAGGGAGTTGACCGCCA CCTGTTTGCGCTGTACATCGTGTCCCGATTCCTCCACCTGCAGTCGCCCTTCCTGACCCAGGTCCATTCG GAGCAGTGGCAGCTGTCCACCAGCCAGATCCCTGTTCAGCAAATGCATCTGTTTGACGTCCACAATTACC CGGACTATGTTTCCTCAGGCGGTGGATTCGGGCCTGCTGATGACCATGGTTATGGTGTTTCTTATATCTT CATGGGGGATGGCATGATCACCTTCCACATCTCCAGCAAAAAATCAAGCACAAAAACGGATTCCCACAGG CTGGGGCAGCACATTGAGGACGCACTGCTGGATGTGGCCTCCCTGTTCCAGGCGGGACAGCATTTTAAGC GCCGGTTCAGAGGGTCAGGGAAGGAGAACTCCAGGCACAGGTGTGGATTTCTCTCCCGCCAGACTGGGGC CTCCAAGGCCTCAATGACATCCACCGACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234902 protein sequence Red=Cloning site Green=Tags(s) XWLKRTRPWASDPR*PRTGLKWNSVPLCCRRSTSLACAPGKGISHVSGMTFSPVCFLPAPSVGFSSSVPS SLPGSSSWILP*D*WRRSKSCCLTGVDNTTGSGGSWQPRCLPRVCGEP*SSHCTWP*GCFCPTTAGFLSP TEPCPPPPRPGWPWSASSLAATRCCSVTSAPCHASPCPLCRTPCASTWSRSGPSSPTRTSTGPRSWRRNS *GCRRRCCSGTCGSSPGGRPIMSVTGGRNLCTCAPEIR*W*TATIT*WTSCMSHPRLCRQLALGMPSMPS SCTATA*TARRYPRLC*WECAPYALPSTRRSSTPRGFQGSKKTTSATSMTANTWLSSTGADSSAWGPTPE TACFPREPWSSSFRESWMIPHRPAPTRNIWQL*QLLPGARGPRCGHP*RPRQRRPWRRWKGPLSLCHWML SPRGSPGRTRQRRWMPTPMLCWPAGAMIAGLTNPSP*SSSLTGSWASAWSTPGPTAPSQDTCGSSLWLQN AFSWATQQTATARGTRTPHYPSPSGCNGTFQTRSTPPSL*P*GEPRSCLKMSTAMSFHSPYLARASSDAA TSLQTASSRSPCNWPTSGTGVNSA*LMSRP*LAYSWKAGRRRCGLARGRPATLSGPWRTKRRRTHSASPC SAWQWTSTRLC*RQP*AGRELTATCLRCTSCPDSSTCSRPS*PRSIRSSGSCPPARSLFSKCICLTSTIT RTMFPQAVDSGLLMTMVMVFLISSWGMA*SPSTSPAKNQAQKRIPTGWGSTLRTHCWMWPPCSRRDSILS AGSEGQGRRTPGTGVDFSPARLGPPRPQ*HPPT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199753
ORF Size	2409 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199753.2
RefSeq Size	2883 bp
RefSeq ORF	2412 bp
Locus ID	126129
UniProt ID	Q8TCG5
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Adipocytokine signaling pathway, Fatty acid metabolism, PPAR signaling pathway
MW	91 kDa
Gene Summary	This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein regulates the beta-oxidation and transport of long-chain fatty acids into mitochondria, and may play a role in the regulation of feeding behavior and whole-body energy homeostasis. Alternatively spliced transcript variants encoding multiple protein isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

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