ITCH (NM_001257138) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ADMFD; AIF4; AIP4; NAPP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234833 representing NM_001257138 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACTTGAAGAAGTAGTTGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGAG ACTTGTCAATTTGTCTTGATGGGCTACAGTTAGAGTCTGAAGTTGTTACCAATGGTGAAACTACATGTTC AGAAAGTGCTTCTCAGAATGATGATGGCTCCAGATCCAAGGATGAAACAAGAGTGAGCACAAATGGATCA GATGACCCTGAAGATGCAGGAGCTGGTGAAAATAGGAGAGTCAGTGGGAATAATTCTCCATCACTCTCAA ATGGTGGTTTTAAACCTTCTAGACCTCCAAGACCTTCACGACCACCACCACCCACCCCACGTAGACCAGC ATCTGTCAATGGTTCACCATCTGCCACTTCTGAAAGTGATGGGTCTAGTACAGGCTCTCTGCCGCCGACA AATACAAATACAAATACATCTGAAGGAGCAACATCTGGATTAATAATTCCTCTTACTATATCTGGAGGCT CAGGCCCTAGGCCATTAAATCCTGTAACTCAAGCTCCCTTGCCACCTGGTTGGGAGCAGAGAGTGGACCA GCACGGGCGAGTTTACTATGTAGATCATGTTGAGAAAAGAACAACATGGGATAGACCAGAACCTCTACCT CCTGGCTGGGAACGGCGGGTTGACAACATGGGACGTATTTATTATGTTGACCATTTCACAAGAACAACAA CGTGGCAGAGGCCAACACTGGAATCCGTCCGGAACTATGAACAATGGCAGCTACAGCGTAGTCAGCTTCA AGGAGCAATGCAGCAGTTTAACCAGAGATTCATTTATGGGAATCAAGATTTATTTGCTACATCACAAAGT AAAGAATTTGATCCTCTTGGTCCATTGCCACCTGGATGGGAGAAGAGAACAGACAGCAATGGCAGAGTAT ATTTCGTCAACCACAACACACGAATTACACAATGGGAAGACCCCAGAAGTCAAGGTCAATTAAATGAAAA GCCCTTACCTGAAGGTTGGGAAATGAGATTCACAGTGGATGGAATTCCATATTTTGTGGACCACAATAGA AGAACTACCACCTATATAGATCCCCGCACAGGAAAATCTGCCCTAGACAATGGACCTCAGATAGCCTATG TTCGGGACTTCAAAGCAAAGGTTCAGTATTTCCGGTTCTGGTGTCAGCAACTGGCCATGCCACAGCACAT AAAGATTACAGTGACAAGAAAAACATTGTTTGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAA GATCTGCGAAGACGTTTGTGGGTGATTTTTCCAGGAGAAGAAGGTTTAGATTATGGAGGTGTAGCAAGAG AATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAATGTATTGCCTGTTTGAATATGCAGGGAAGGA TAACTACTGCTTGCAGATAAACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTTTCGTTTTATT GGCAGATTTATTGCCATGGCTCTGTTCCATGGGAAATTCATAGACACGGGTTTTTCTTTACCATTCTATA AGCGTATCTTGAACAAACCAGTTGGACTCAAGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCT CATCTGGGTTAAGGAAAACAATATTGAGGAATGTGATTTGGAAATGTACTTCTCCGTTGACAAAGAAATT CTAGGTGAAATTAAGAGTCATGATCTGAAACCTAATGGTGGCAATATTCTTGTAACAGAAGAAAATAAAG AGGAATACATCAGAATGGTAGCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTT TGAAGGCTTTAATGAAATTCTTCCCCAGCAATATTTGCAATACTTTGATGCAAAGGAATTAGAGGTCCTT TTATGTGGAATGCAAGAGATTGATTTGAATGACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGA CCAGCAAACAAATCATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGAATGAGACTTCT GCAGTTTGTTACTGGAACCTGCCGATTGCCAGTAGGAGGATTTGCTGATCTCATGGGGAGCAATGGACCA CAGAAATTCTGCATTGAAAAAGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCTGTTTTAATCGCC TGGACCTGCCACCATACAAGAGCTATGAGCAACTGAAGGAAAAGCTGTTGTTTGCCATAGAAGAAACAGA AGGATTTGGACAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234833 representing NM_001257138 Red=Cloning site Green=Tags(s) MKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSESASQNDDGSRSKDETRVSTNGS DDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPT NTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLP PGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQS KEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNR RTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQ DLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFI GRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEI LGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVL LCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGP QKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001257138
ORF Size	2256 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001257138.3
RefSeq Size	6270 bp
RefSeq ORF	2259 bp
Locus ID	83737
UniProt ID	Q96J02
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Endocytosis, Ubiquitin mediated proteolysis
MW	86.9 kDa
Gene Summary	This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

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