PTPDC1 (NM_001253830) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PTP9Q22
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234825 representing NM_001253830 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGGAGTCTTGCCTCAGAATGAACAACCATATTCTACCTTGGTGAATAACAGCGAGTGTGTTG CAAACATGAAAGGAAATTTAGAACGTCCAACACCAAAGTACACAAAAGTAGGGGAGCGTTTACGGCATGT CATTCCTGGACACATGGCATGTTCCATGGCGTGTGGCGGTAGAGCTTGCAAGTATGAGAACCCAGCCCGC TGGAGTGAGCAGGAGCAAGCCATTAAGGGGGTTTACTCATCCTGGGTCACTGATAATATACTGGCCATGG CCCGCCCATCCTCTGAGCTCCTGGAGAAGTACCACATCATTGATCAGTTCCTCAGCCATGGCATAAAAAC AATAATCAACCTCCAGCGCCCTGGTGAGCATGCTAGCTGTGGGAACCCTCTGGAACAAGAAAGTGGCTTC ACATACCTTCCTGAGGCTTTCATGGAGGCTGGCATTTACTTCTACAATTTCGGATGGAAGGATTATGGTG TAGCGTCTCTTACTACTATCCTAGATATGGTGAAGGTGATGACATTTGCCTTACAGGAAGGAAAAGTAGC TATCCATTGTCATGCAGGGCTTGGTCGAACAGGTGTTTTAATAGCCTGTTACTTAGTTTTTGCAACGAGA ATGACTGCTGACCAAGCAATTATATTTGTGCGGGCAAAGCGACCCAATTCCATACAAACCAGAGGACAGC TCCTCTGTGTAAGGGAATTTACTCAGTTTCTAACTCCTCTCCGCAATATATTCTCTTGCTGTGATCCCAA AGCACATGCTGTCACCTTACCTCAATATCTAATTCGCCAGCGTCATCTGCTTCATGGTTATGAGGCACGA CTTCTGAAACACGTGCCAAAAATTATCCACCTAGTTTGCAAATTGCTGCTGGACTTAGCGGAGAACAGGC CAGTGATGATGAAGGATGTGTCCGAAGGACCTGGTCTCTCTGCTGAAATAGAAAAGACAATGTCTGAGAT GGTCACCATGCAGCTGGATAAAGAGTTACTGAGGCATGACAGTGATGTGTCCAACCCGCCTAACCCCACT GCAGTGGCAGCAGATTTTGACAATCGAGGCATGATTTTCTCCAATGAGCAACAGTTTGACCCTCTTTGGA AAAGGCGGAATGTTGAGTGCCTTCAACCCCTGACTCATCTGAAAAGGCGGCTCAGCTACAGTGACTCAGA TTTAAAGAGGGCCGAGAACCTCCTGGAGCAAGGGGAGACTCCACAGACAGTGCCTGCCCAGATCTTGGTT GGCCACAAGCCCAGGCAGCAGAAGCTCATAAGCCATTGTTACATCCCACAGTCTCCAGAACCAGACTTAC ACAAGGAAGCCTTGGTTCGCAGCACACTTTCTTTCTGGAGTCAGTCAAAGTTTGGAGGCCTGGAAGGACT CAAAGATAATGGGTCACCAATTTTCCATGGAAGGATCATTCCAAAGGAAGCACAGCAGAGTGGAGCTTTC TCTGCAGATGTTTCAGGCTCACACAGCCCTGGGGAGCCAGTTTCACCCAGCTTTGCAAATGTCCATAAGG ATCCAAACCCTGCTCACCAGCAAGTGTCTCACTGTCAGTGTAAAACTCATGGTGTTGGGAGCCCTGGCTC TGTCAGGCAGAACAGCAGGACACCCCGAAGCCCTCTGGACTGTGGCTCCAGTCCCAAAGCACAGTTCTTG GTTGAACATGAAACCCAGGACAGTAAAGATCTGTCTGAAGCAGCTTCACACTCTGCATTACAGTCTGAAT TGAGTGCTGAGGCAAGAAGAATACTGGCGGCCAAAGCCCTAGCAAATTTAAATGAATCTGTAGAAAAGGA GGAACTAAAAAGGAAGAAAGAGCTTAATTCCCGAGATGGAGCTTGGGAAAGAATATGTGGCGAGAGGGAC CCTTTCATCCTATGCAGCTTGATGTGGTCTTGGGTGGAGCAACTGAAGGAGCCTGTAATCACCAAAGAGG ATGTGGACATGTTGGTTGACAGGCGAGCAGATGCCGCAGAAGCACTTTTTTTATTAGAGAAGGGACAGCA CCAGACTATTCTCTGCGTGTTGCACTGCATAGTGAACCTGCAGACAATTCCCGTGGATGTGGAGGAAGCT TTCCTTGCCCATGCCATTAAGGCATTCACTAAGGTTAATTTTGATTCTGAAAATGGACCAACAGTTTACA ACACCCTGAAGAAAATATTTAAGCACACGCTGGAAGAAAAAAGAAAAATGACAAAAGATGGCCCTAAGCC TGGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234825 representing NM_001253830 Red=Cloning site Green=Tags(s) MAAGVLPQNEQPYSTLVNNSECVANMKGNLERPTPKYTKVGERLRHVIPGHMACSMACGGRACKYENPAR WSEQEQAIKGVYSSWVTDNILAMARPSSELLEKYHIIDQFLSHGIKTIINLQRPGEHASCGNPLEQESGF TYLPEAFMEAGIYFYNFGWKDYGVASLTTILDMVKVMTFALQEGKVAIHCHAGLGRTGVLIACYLVFATR MTADQAIIFVRAKRPNSIQTRGQLLCVREFTQFLTPLRNIFSCCDPKAHAVTLPQYLIRQRHLLHGYEAR LLKHVPKIIHLVCKLLLDLAENRPVMMKDVSEGPGLSAEIEKTMSEMVTMQLDKELLRHDSDVSNPPNPT AVAADFDNRGMIFSNEQQFDPLWKRRNVECLQPLTHLKRRLSYSDSDLKRAENLLEQGETPQTVPAQILV GHKPRQQKLISHCYIPQSPEPDLHKEALVRSTLSFWSQSKFGGLEGLKDNGSPIFHGRIIPKEAQQSGAF SADVSGSHSPGEPVSPSFANVHKDPNPAHQQVSHCQCKTHGVGSPGSVRQNSRTPRSPLDCGSSPKAQFL VEHETQDSKDLSEAASHSALQSELSAEARRILAAKALANLNESVEKEELKRKKELNSRDGAWERICGERD PFILCSLMWSWVEQLKEPVITKEDVDMLVDRRADAAEALFLLEKGQHQTILCVLHCIVNLQTIPVDVEEA FLAHAIKAFTKVNFDSENGPTVYNTLKKIFKHTLEEKRKMTKDGPKPGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001253830
ORF Size	2247 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253830.2
RefSeq Size	4497 bp
RefSeq ORF	2250 bp
Locus ID	138639
UniProt ID	A2A3K4
Protein Families	Druggable Genome, Phosphatase
MW	84.2 kDa
Gene Summary	The protein encoded by this gene contains a characteristic motif of protein tyrosine phosphatases (PTPs). PTPs regulate activities of phosphoproteins through dephosphorylation. They are signaling molecules involved in the regulation of a wide variety of biological processes. The specific function of this protein has not yet been determined. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

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