CTAGE5 (MIA2) (NM_001247990) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CTAGE5; MEA6; MGEA; MGEA6; MGEA11; TALI
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234787 representing NM_001247990 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTTCTGGACTAATTGAAGAAAAAAGTAAACTACTTGAAAAATTTAGCCTTGTTCAAAAAGAGTATG AAGGCTATGAAGTAGAGTCATCTTTAAAGGATGCCAGCTTTGAGAAGGAGGCAACAGAAGCACAAAGTTT GGAGGTAGAAAATCAAATGGCAACCTGTGAAAAGCTGAACAGGTCCAATTCTGAACTTGAGGATGAAATA CTCTGTCTAGAAAAAGAGTTAAAAGAAGAGAAATCCAAACATTCTGAACAAGATGAATTGATGGCGGATA TTTCAAAAAGGATACAGTCTCTAGAAGATGAGTCAAAATCCCTCAAATCACAAGTAGCTGAAGCCAAAAT GACCTTCAAGATATTTCAAATGAATGAAGAACGACTGAAGATAGCAATAAAAGATGCTTTGAATGAAAAT TCTCAACTTCAGGAAAGCCAGAAACAGCTTTTGCAAGAAGCTGAAGTATGGAAAGAACAAGTGAGTGAAC TTAATAAACAGAAAGTAACATTTGAAGACTCCAAAGTACATGCAGAACAAGTTCTAAATGATAAAGAAAG TCACATCAAGACTCTGACTGAACGCTTGTTAAAGATGAAAGATTGGGCTGCTATGCTTGGAGAAGACATA ACGGATGATGATAACTTGGAATTAGAAATGAACAGTGAATCGGAAAATGGTGCTTACTTAGATAATCCTC CAAAAGGAGCTTTGAAGAAACTGATTCATGCTGCTAAGTTAAATGCTTCTTTAAAAACCTTAGAAGGAGA AAGAAACCAAATTTATATTCAGTTGTCTGAAGTTGATAAAACAAAGGAAGAGCTTACAGAGCATATTAAA AATCTTCAGACTGAACAAGCATCTTTGCAGTCAGAAAACACACATTTTGAAAATGAGAATCAGAAGCTTC AACAGAAACTTAAAGTAATGACTGAATTATATCAAGAAAATGAAATGAAACTCCACAGGAAATTAACAGT AGAGGAAAATTATCGGTTAGAGAAAGAAGAGAAACTTTCTAAAGTAGATGAAAAGATCAGCCATGCCACT GAAGAGCTGGAGACCTATAGAAAGCGAGCCAAAGATCTTGAAGAAGAATTGGAGAGAACTATTCATTCTT ATCAAGGGCAGATTATTTCCCATGAGAAAAAAGCACATGATAATTGGTTGGCAGCTCGGAATGCTGAAAG AAACCTCAATGATTTAAGGAAAGAAAATGCTCACAACAGACAAAAATTAACTGAAACAGAGCTTAAATTT GAACTTTTAGAAAAAGATCCTTATGCACTCGATGTTCCAAATACAGCATTTGGCAGAGAGCATTCCCCAT ATGGTCCCTCACCATTGGGTTGGCCTTCATCTGAAACAAGAGCTTTTCTCTCTCCTCCAACTTTGTTGGA GGGTCCACTCAGACTCTCACCTTTGCTTCCAGGGGGAGGAGGAAGAGGCTCACGAGGCCCAGGGAATCCT CTGGACCATCAGATTACCAATGAAAGAGGAGAATCAAGCTGTGATAGGTTAACCGATCCTCATAGGGCTC CCTCTGACACTGGGTCTCTGTCACCTCCATGGGACCAGGACCGTAGGATGATGTTTCCTCCGCCAGGACA ATCATATCCTGATTCAGCCCTTCCTCCACAAAGGCAAGACAGATTTTGTTCTAATTCTGGTAGACTGTCT GGACCAGCAGAACTCAGAAGTTTTAATATGCCTTCTTTGGATAAAATGGATGGGTCAATGCCTTCAGAAA TGGAATCCAGTAGAAATGATACCAAAGATGATCTTGGTAATTTAAATGTGCCTGATTCATCTCTCCCTGC TGAAAATGAAGCCACTGGCCCTGGCTTTGTTCCTCCACCTCTTGCTCCAATCAGAGGTCCATTGTTTCCA GTGGATGCAAGAGGCCCATTCTTGAGAAGAGGACCTCCTTTCCCCCCACCTCCTCCAGGAGCCATGTTTG GAGCTTCTCGAGATTATTTTCCACCAGGGGATTTCCCAGGTCCACCACCTGCTCCATTTGCAATGAGAAA TGTCTATCCACCGAGGGGTTTTCCTCCTTACCTTCCCCCAAGACCTGGATTTTTCCCCCCACCCCCACAT TCTGAAGGTAGAAGTGAGTTCCCCTCAGGTTTGATTCCACCTTCAAATGAGCCTGCTACTGAACATCCAG AACCACAGCAAGAAACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234787 representing NM_001247990 Red=Cloning site Green=Tags(s) MLSGLIEEKSKLLEKFSLVQKEYEGYEVESSLKDASFEKEATEAQSLEVENQMATCEKLNRSNSELEDEI LCLEKELKEEKSKHSEQDELMADISKRIQSLEDESKSLKSQVAEAKMTFKIFQMNEERLKIAIKDALNEN SQLQESQKQLLQEAEVWKEQVSELNKQKVTFEDSKVHAEQVLNDKESHIKTLTERLLKMKDWAAMLGEDI TDDDNLELEMNSESENGAYLDNPPKGALKKLIHAAKLNASLKTLEGERNQIYIQLSEVDKTKEELTEHIK NLQTEQASLQSENTHFENENQKLQQKLKVMTELYQENEMKLHRKLTVEENYRLEKEEKLSKVDEKISHAT EELETYRKRAKDLEEELERTIHSYQGQIISHEKKAHDNWLAARNAERNLNDLRKENAHNRQKLTETELKF ELLEKDPYALDVPNTAFGREHSPYGPSPLGWPSSETRAFLSPPTLLEGPLRLSPLLPGGGGRGSRGPGNP LDHQITNERGESSCDRLTDPHRAPSDTGSLSPPWDQDRRMMFPPPGQSYPDSALPPQRQDRFCSNSGRLS GPAELRSFNMPSLDKMDGSMPSEMESSRNDTKDDLGNLNVPDSSLPAENEATGPGFVPPPLAPIRGPLFP VDARGPFLRRGPPFPPPPPGAMFGASRDYFPPGDFPGPPPAPFAMRNVYPPRGFPPYLPPRPGFFPPPPH SEGRSEFPSGLIPPSNEPATEHPEPQQET TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001247990
ORF Size	2187 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001247990.2
RefSeq Size	3585 bp
RefSeq ORF	2190 bp
Locus ID	4253
UniProt ID	Q96PC5
Protein Families	Transmembrane
MW	82.9 kDa
Gene Summary	This gene encodes s receptor in the endoplasmic reticulum, which plays a role in the export of large pre-chylomicrons and pre-very low density lipoproteins (pre-VLDLs). Three major classes of transcripts are generated from this gene- melanoma inhibitory activity 2-specific transcripts, cTAGE family member 5-specific transcripts and transcripts that include exons from both these transcript species (TANGO1-like or TALI). Additionally, alternative splicing in these transcripts results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Sep 2016]

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