Metabotropic Glutamate Receptor 4 (GRM4) (NM_001256810) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GPRC1D; mGlu4; MGLUR4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234544 representing NM_001256810 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCTGACAGCTGGGGCTCCAAGATTGCACCTGTGCTGCACCTGGAGGAGGTGGCTGAGGGTGCTG TCACGATCCTCCCCAAGAGGATGTCCGTACGAGGCTTCGACCGCTACTTCTCCAGCCGCACGCTGGACAA CAACCGGCGCAACATCTGGTTTGCCGAGTTCTGGGAGGACAACTTCCACTGCAAGCTGAGCCGCCACGCC CTCAAGAAGGGCAGCCACGTCAAGAAGTGCACCAACCGTGAGCGAATTGGGCAGGATTCAGCTTATGAGC AGGAGGGGAAGGTGCAGTTTGTGATCGATGCCGTGTACGCCATGGGCCACGCGCTGCACGCCATGCACCG TGACCTGTGTCCCGGCCGCGTGGGGCTCTGCCCGCGCATGGACCCTGTAGATGGCACCCAGCTGCTTAAG TACATCCGAAACGTCAACTTCTCAGGCATCGCAGGGAACCCTGTGACCTTCAATGAGAATGGAGATGCGC CTGGGCGCTATGACATCTACCAATACCAGCTGCGCAACGATTCTGCCGAGTACAAGGTCATTGGCTCCTG GACTGACCACCTGCACCTTAGAATAGAGCGGATGCACTGGCCGGGGAGCGGGCAGCAGCTGCCCCGCTCC ATCTGCAGCCTGCCCTGCCAACCGGGTGAGCGGAAGAAGACAGTGAAGGGCATGCCTTGCTGCTGGCACT GCGAGCCTTGCACAGGGTACCAGTACCAGGTGGACCGCTACACCTGTAAGACGTGTCCCTATGACATGCG GCCCACAGAGAACCGCACGGGCTGCCGGCCCATCCCCATCATCAAGCTTGAGTGGGGCTCGCCCTGGGCC GTGCTGCCCCTCTTCCTGGCCGTGGTGGGCATCGCTGCCACGTTGTTCGTGGTGATCACCTTTGTGCGCT ACAACGACACGCCCATCGTCAAGGCCTCGGGCCGTGAACTGAGCTACGTGCTGCTGGCAGGCATCTTCCT GTGCTATGCCACCACCTTCCTCATGATCGCTGAGCCCGACCTTGGCACCTGCTCGCTGCGCCGAATCTTC CTGGGACTAGGGATGAGCATCAGCTATGCAGCCCTGCTCACCAAGACCAACCGCATCTACCGCATCTTCG AGCAGGGCAAGCGCTCGGTCAGTGCCCCACGCTTCATCAGCCCCGCCTCACAGCTGGCCATCACCTTCAG CCTCATCTCGCTGCAGCTGCTGGGCATCTGTGTGTGGTTTGTGGTGGACCCCTCCCACTCGGTGGTGGAC TTCCAGGACCAGCGGACACTCGACCCCCGCTTCGCCAGGGGTGTGCTCAAGTGTGACATCTCGGACCTGT CGCTCATCTGCCTGCTGGGCTACAGCATGCTGCTCATGGTCACGTGCACCGTGTATGCCATCAAGACACG CGGCGTGCCCGAGACCTTCAATGAGGCCAAGCCCATTGGCTTCACCATGTACACCACTTGCATCGTCTGG CTGGCCTTCATCCCCATCTTCTTTGGCACCTCGCAGTCGGCCGACAAGCTGTACATCCAGACGACGACGC TGACGGTCTCGGTGAGTCTGAGCGCCTCGGTGTCCCTGGGAATGCTCTACATGCCCAAAGTCTACATCAT CCTCTTCCACCCGGAGCAGAACGTGCCCAAGCGCAAGCGCAGCCTCAAAGCCGTCGTTACGGCGGCCACC ATGTCCAACAAGTTCACGCAGAAGGGCAACTTCCGGCCCAACGGAGAGGCCAAGTCTGAGCTCTGCGAGA ACCTTGAGGCCCCAGCGCTGGCCACCAAACAGACTTACGTCACTTACACCAACCATGCAATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234544 representing NM_001256810 Red=Cloning site Green=Tags(s) MGSDSWGSKIAPVLHLEEVAEGAVTILPKRMSVRGFDRYFSSRTLDNNRRNIWFAEFWEDNFHCKLSRHA LKKGSHVKKCTNRERIGQDSAYEQEGKVQFVIDAVYAMGHALHAMHRDLCPGRVGLCPRMDPVDGTQLLK YIRNVNFSGIAGNPVTFNENGDAPGRYDIYQYQLRNDSAEYKVIGSWTDHLHLRIERMHWPGSGQQLPRS ICSLPCQPGERKKTVKGMPCCWHCEPCTGYQYQVDRYTCKTCPYDMRPTENRTGCRPIPIIKLEWGSPWA VLPLFLAVVGIAATLFVVITFVRYNDTPIVKASGRELSYVLLAGIFLCYATTFLMIAEPDLGTCSLRRIF LGLGMSISYAALLTKTNRIYRIFEQGKRSVSAPRFISPASQLAITFSLISLQLLGICVWFVVDPSHSVVD FQDQRTLDPRFARGVLKCDISDLSLICLLGYSMLLMVTCTVYAIKTRGVPETFNEAKPIGFTMYTTCIVW LAFIPIFFGTSQSADKLYIQTTTLTVSVSLSASVSLGMLYMPKVYIILFHPEQNVPKRKRSLKAVVTAAT MSNKFTQKGNFRPNGEAKSELCENLEAPALATKQTYVTYTNHAI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256810
ORF Size	1812 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256810.1, NP_001243739.1
RefSeq Size	3078 bp
RefSeq ORF	1814 bp
Locus ID	2914
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction, Taste transduction
MW	68.4 kDa
Gene Summary	L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.