KAT7 (NM_001199157) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HBO1; HBOA; MYST2; ZC2HC7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234297 representing NM_001199157 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCGAAGGAAGAGGAATGCAGGCAGTAGTTCAGATGGAACCGAAGATTCCGATTTTTCTACAGATC TCGAGCACACAGACAGTTCAGAAAGTGATGGCACATCCCGACGATCTGCTCGAGTCACCCGCTCCTCAGC CAGGCTAAGCCAGAGTTCTCAAGATTCCAGTCCTGTTCGAAATCTGCAGTCTTTTGGCACTGAGGAGCCT GCTTACTCTACCAGAAGAGTGACCCGTAGTCAGCAGCAGCCTACCCCAGTGACACCGAAAAAATACCCTC TTCGGCAGACTCGTTCATCTGGTTCAGAAACTGAGCAAGTGGTTGATTTTTCAGATAGAGGACACCTTAC AGGAAAACATGAGAGACATTTCTCCATCTCAGGATGCCCACTGTATCATAACCTCTCAGCTGACGAATGC AAGGCACCAACGGAGAGGCAGCTTCGATATAAGGAAAAAGTGGCTGAACTCAGGAAGAAAAGAAATTCTG GACTGAGCAAAGAACAGAAAGAGAAATATATGGAACACAGACAGACCTATGGGAACACACGGGAACCTCT TTTAGAAAACCTGACAAGCGAGTATGACTTGGATCTTTTCCGAAGAGCACAAGCCCGGGCTTCAGAGGAT TTGGAGAAGTTAAGGCTGCAAGGCCAAATCACAGAGGGAAGCAACATGATTAAAACAATTGCTTTTGGCC GCTATGAGCTTGATACCTGGTATCATTCTCCATATCCTGAAGAATATGCACGGCTGGGACGTCTCTATAT GTGTGAATTCTGTTTAAAATATATGAAGAGCCAAACGATACTCCGCCGGCACATGGCCAAATGTGTGTGG AAACACCCACCTGGTGATGAGATATATCGCAAAGGTTCAATCTCTGTGTTTGAAGTGGATGGCAAGAAAA ACAAGATCTACTGCCAAAACCTGTGCCTGTTGGCCAAACTTTTTCTGGACCACAAGACATTATATTATGA TGTGGAGCCCTTCCTGTTCTATGTTATGACAGAGGCGGACAACACTGGCTGTCACCTGATTGGATATTTT TCTAAGGAAAAGAATTCATTCCTCAACTACAACGTCTCCTGTATCCTTACTATGCCTCAGTACATGAGAC AGGGCTATGGCAAGATGCTTATTGATTTCAGTTATTTGCTTTCCAAAGTCGAAGAAAAAGTTGGCTCCCC AGAACGTCCACTCTCAGATCTGGGGCTTATAAGCTATCGCAGTTACTGGAAAGAAGTACTTCTCCGCTAC CTGCATAATTTTCAAGGCAAAGAGATTTCTATCAAAGAAATCAGTCAGGAGACGGCTGTGAATCCTGTGG ACATTGTCAGCACTCTGCAAGCCCTTCAGATGCTCAAATACTGGAAGGGAAAACACCTAGTTTTAAAGAG ACAGGACCTGATTGATGAGTGGATAGCCAAAGAGGCCAAAAGGTCCAACTCCAATAAAACCATGGATCCC AGCTGCTTAAAATGGACCCCTCCCAAGGGCACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234297 representing NM_001199157 Red=Cloning site Green=Tags(s) MPRRKRNAGSSSDGTEDSDFSTDLEHTDSSESDGTSRRSARVTRSSARLSQSSQDSSPVRNLQSFGTEEP AYSTRRVTRSQQQPTPVTPKKYPLRQTRSSGSETEQVVDFSDRGHLTGKHERHFSISGCPLYHNLSADEC KAPTERQLRYKEKVAELRKKRNSGLSKEQKEKYMEHRQTYGNTREPLLENLTSEYDLDLFRRAQARASED LEKLRLQGQITEGSNMIKTIAFGRYELDTWYHSPYPEEYARLGRLYMCEFCLKYMKSQTILRRHMAKCVW KHPPGDEIYRKGSISVFEVDGKKNKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVMTEADNTGCHLIGYF SKEKNSFLNYNVSCILTMPQYMRQGYGKMLIDFSYLLSKVEEKVGSPERPLSDLGLISYRSYWKEVLLRY LHNFQGKEISIKEISQETAVNPVDIVSTLQALQMLKYWKGKHLVLKRQDLIDEWIAKEAKRSNSNKTMDP SCLKWTPPKGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199157
ORF Size	1503 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199157.1, NP_001186086.1
RefSeq Size	3334 bp
RefSeq ORF	1506 bp
Locus ID	11143
UniProt ID	O95251
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
MW	58.6 kDa
Gene Summary	The protein encoded by this gene is part of the multimeric HBO1 complex, which possesses histone H4-specific acetyltransferase activity. This activity is required for functional replication origins and is involved in transcriptional activation of some genes. In both cases, the acetylation of histone H4 helps unfold chromatin so that the DNA can be accessed and replicated or transcribed. [provided by RefSeq, Oct 2016]

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