GPR176 (NM_001271855) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HB-954
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234199 representing NM_001271855 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCTCCACTGCTTCTTATTTCTAGAACTTTGGGAAACTTCATGGTGTTATGGTCAACTTGCCGCA CAACCGTGTTCAAATCTGTCACCAACAGGTTCATTAAAAACCTGGCCTGCTCGGGGATTTGTGCCAGCCT GGTCTGTGTGCCCTTCGACATCATCCTCAGCACCAGTCCTCACTGTTGCTGGTGGATCTACACCATGCTC TTCTGCAAGGTCGTCAAATTTTTGCACAAAGTATTCTGCTCTGTGACCATCCTCAGCTTCCCTGCTATTG CTTTGGACAGGTACTACTCAGTCCTCTATCCACTGGAGAGGAAAATATCTGATGCCAAGTCCCGTGAACT GGTGATGTACATCTGGGCCCATGCAGTGGTGGCCAGTGTCCCTGTGTTTGCAGTAACCAATGTGGCTGAC ATCTATGCCACGTCCACCTGCACGGAAGTCTGGAGCAACTCCTTGGGCCACCTGGTGTACGTTCTGGTGT ATAACATCACCACGGTCATTGTGCCTGTGGTGGTGGTGTTCCTCTTCTTGATACTGATCCGACGGGCCCT GAGTGCCAGCCAGAAGAAGAAGGTCATCATAGCAGCGCTCCGGACCCCACAGAACACCATCTCTATTCCC TATGCCTCCCAGCGGGAGGCCGAGCTGCACGCCACCCTGCTCTCCATGGTGATGGTCTTCATCTTGTGTA GCGTGCCCTATGCCACCCTGGTCGTCTACCAGACTGTGCTCAATGTCCCTGACACTTCCGTCTTCTTGCT GCTCACTGCTGTTTGGCTGCCCAAAGTCTCCCTGCTGGCAAACCCTGTTCTCTTTCTTACTGTGAACAAA TCTGTCCGCAAGTGCTTGATAGGGACCCTGGTGCAACTACACCACCGGTACAGTCGCCGTAATGTGGTCA GTACAGGGAGTGGCATGGCTGAGGCCAGCCTGGAACCCAGCATACGCTCGGGTAGCCAGCTCCTGGAGAT GTTCCACATTGGGCAGCAGCAGATCTTTAAGCCCACAGAGGATGAGGAAGAGAGTGAGGCCAAGTACATT GGCTCAGCTGACTTCCAGGCCAAGGAGATATTTAGCACCTGCCTGGAGGGAGAGCAGGGGCCACAGTTTG CGCCCTCTGCCCCACCCCTGAGCACAGTGGACTCTGTATCCCAGGTGGCACCGGCAGCCCCTGTGGAACC TGAAACATTCCCTGATAAGTATTCCCTGCAGTTTGGCTTTGGGCCTTTTGAGTTGCCTCCTCAGTGGCTC TCAGAGACCCGAAACAGCAAGAAGCGGCTGCTTCCCCCCTTGGGCAACACCCCAGAAGAGCTGATCCAGA CAAAGGTGCCCAAGGTAGGCAGGGTGGAGCGGAAGATGAGCAGAAACAATAAAGTGAGCATTTTTCCAAA GGTGGATTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234199 representing NM_001271855 Red=Cloning site Green=Tags(s) MPAPLLLISRTLGNFMVLWSTCRTTVFKSVTNRFIKNLACSGICASLVCVPFDIILSTSPHCCWWIYTML FCKVVKFLHKVFCSVTILSFPAIALDRYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAVTNVAD IYATSTCTEVWSNSLGHLVYVLVYNITTVIVPVVVVFLFLILIRRALSASQKKKVIIAALRTPQNTISIP YASQREAELHATLLSMVMVFILCSVPYATLVVYQTVLNVPDTSVFLLLTAVWLPKVSLLANPVLFLTVNK SVRKCLIGTLVQLHHRYSRRNVVSTGSGMAEASLEPSIRSGSQLLEMFHIGQQQIFKPTEDEEESEAKYI GSADFQAKEIFSTCLEGEQGPQFAPSAPPLSTVDSVSQVAPAAPVEPETFPDKYSLQFGFGPFELPPQWL SETRNSKKRLLPPLGNTPEELIQTKVPKVGRVERKMSRNNKVSIFPKVDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271855
ORF Size	1410 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271855.2
RefSeq Size	3700 bp
RefSeq ORF	1413 bp
Locus ID	11245
UniProt ID	Q14439
Protein Families	Druggable Genome, GPCR, Transmembrane
MW	52.8 kDa
Gene Summary	Members of the G protein-coupled receptor family, such as GPR176, are cell surface receptors involved in responses to hormones, growth factors, and neurotransmitters (Hata et al., 1995 [PubMed 7893747]).[supplied by OMIM, Jul 2008]

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