DEF8 (NM_001242820) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234137 representing NM_001242820 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAATATGATGAGAAGCTGGCCCGTTTCCGGCAGGCCCACCTCAACCCCTTCAACAAGCAGTCTGGGC CGAGACAGCATGAGCAGGGCCCTGGGGAGGAGGTCCCGGACGTCACTCCTGAAGAGGCCCTGCCTGAGCT GCCCCCTGGGGAGCCGGAATTCCGCTGCCCTGAACGCGTGATGGATCTCGGCCTGTCTGAGGACCACTTC TCCCGCCCTGTGGGTCTGTTCCTGGCCTCTGACGTCCAGCAGCTGCGGCAGGCGATCGAGGAGTGCAAGC AGGTGATTCTGGAGCTGCCCGAGCAGTCGGAGAAGCAGAAGGATGCCGTGGTGCGACTCATCCACCTCCG GCTGAAGCTCCAGGAGCTGAAGGACCCCAATGAGGATGAGCCAAACATCCGAGTGCTCCTTGAGCACCGC TTTTACAAGGAGAAGAGCAAGAGCGTCAAGCAGACCTGTGACAAGTGTAACACCATCATCTGGGGGCTCA TTCAGACCTGGTACACCTGCACAGGGTGTTATTACCGCTGTCACAGTAAGTGCTTGAACCTCATCTCCAA GCCCTGTGTGAGCTCCAAAGTCAGCCACCAAGCTGAATACGAACTGAACATCTGCCCTGAGACAGGGCTG GACAGCCAGGATTACCGCTGTGCCGAGTGCCGGGCGCCCATCTCTCTGCGGGGTGTGCCCAGTGAGGCCA GGCAGTGCGACTACACCGGCCAGTACTACTGCAGCCACTGCCACTGGAACGACCTGGCTGTGATCCCTGC ACGCGTTGTACACAACTGGGACTTTGAGCCTCGAAAGGTTTCTCGCTGCAGCATGCGCTACCTGGCGCTG ATGGTGTCTCGGCCCGTACTCAGGCTCCGGGAGATCAACCCTCTGCTGTTCAGCTACGTGGAGGAGCTGG TGGAGATTCGCAAGCTGCGCCAGGACATCCTGCTCATGAAGCCGTACTTCATCACCTGCAGGGAGGCCAT GGAGGCTCGTCTGCTGCTGCAGCTCCAGGATCGGCAGCATTTTGTGGAGAACGACGAGATGTACTCTGTC CAGGACCTCCTGGACGTGCATGCCGGCCGCCTGGGCTGCTCGCTCACCGAGATCCACACGCTCTTCGCCA AGCACATCAAGCTGGACTGCGAGCGGTGCCAGGCCAAGGGCTTCGTGTGTGAGCTCTGCAGAGAGGGCGA CGTGCTGTTCCCGTTCGACAGCCACACGTCTGTGTGCGCCGACTGCTCCGCGGTCTTCCACAGGGACTGC TACTACGACAACTCCACCACTTGTCCCAAGTGTGCCCGGCTCAGCCTGAGGAAGCAGTCGCTCTTCCAGG AGCCAGGTCCCGATGTGGAGGCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234137 representing NM_001242820 Red=Cloning site Green=Tags(s) MEYDEKLARFRQAHLNPFNKQSGPRQHEQGPGEEVPDVTPEEALPELPPGEPEFRCPERVMDLGLSEDHF SRPVGLFLASDVQQLRQAIEECKQVILELPEQSEKQKDAVVRLIHLRLKLQELKDPNEDEPNIRVLLEHR FYKEKSKSVKQTCDKCNTIIWGLIQTWYTCTGCYYRCHSKCLNLISKPCVSSKVSHQAEYELNICPETGL DSQDYRCAECRAPISLRGVPSEARQCDYTGQYYCSHCHWNDLAVIPARVVHNWDFEPRKVSRCSMRYLAL MVSRPVLRLREINPLLFSYVEELVEIRKLRQDILLMKPYFITCREAMEARLLLQLQDRQHFVENDEMYSV QDLLDVHAGRLGCSLTEIHTLFAKHIKLDCERCQAKGFVCELCREGDVLFPFDSHTSVCADCSAVFHRDC YYDNSTTCPKCARLSLRKQSLFQEPGPDVEA SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242820
ORF Size	1353 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242820.1, NP_001229749.1
RefSeq Size	3902 bp
RefSeq ORF	1356 bp
Locus ID	54849
UniProt ID	Q6ZN54
MW	52.7 kDa
Gene Summary	Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts. Involved in bone resorption.[UniProtKB/Swiss-Prot Function]

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