NOS1 (NM_001204214) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233200 representing NM_001204214 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCCATCATGCATCCTTCTCAGCATGCAAGGAGGCCTGAAGACGTCCGCACAAAAGGACAGCTCT TCCCTCTCGCCAAAGAGTTTATTGATCAATACTATTCATCAATTAAAAGATTTGGCTCCAAAGCCCACAT GGAAAGGCTGGAAGAGGTGAACAAAGAGATCGACACCACTAGCACTTACCAGCTCAAGGACACAGAGCTC ATCTATGGGGCCAAGCACGCCTGGCGGAATGCCTCGCGCTGTGTGGGCAGGATCCAGTGGTCCAAGCTGC AGGTATTCGATGCCCGTGACTGCACCACGGCCCACGGGATGTTCAACTACATCTGTAACCATGTCAAGTA TGCCACCAACAAAGGGAACCTCAGGTCTGCCATCACCATATTCCCCCAGAGGACAGACGGCAAGCACGAC TTCCGAGTCTGGAACTCCCAGCTCATCCGCTACGCTGGCTACAAGCAGCCTGACGGCTCCACCCTGGGGG ACCCAGCCAATGTGCAGTTCACAGAGATATGCATACAGCAGGGCTGGAAACCGCCTAGAGGCCGCTTCGA TGTCCTGCCGCTCCTGCTTCAGGCCAACGGCAATGACCCTGAGCTCTTCCAGATTCCTCCAGAGCTGGTG TTGGAAGTTCCCATCAGGCACCCCAAGTTTGAGTGGTTCAAGGACCTGGGGCTGAAGTGGTACGGCCTCC CCGCCGTGTCCAACATGCTCCTAGAGATTGGCGGCCTGGAGTTCAGCGCCTGTCCCTTCAGTGGCTGGTA CATGGGCACAGAGATTGGTGTCCGCGACTACTGTGACAACTCCCGCTACAATATCCTGGAGGAAGTGGCC AAGAAGATGAACTTAGACATGAGGAAGACGTCCTCCCTGTGGAAGGACCAGGCGCTGGTGGAGATCAATA TCGCGGTTCTCTATAGCTTCCAGAGTGACAAAGTGACCATTGTTGACCATCACTCCGCCACCGAGTCCTT CATTAAGCACATGGAGAATGAGTACCGCTGCCGGGGGGGCTGCCCTGCCGACTGGGTGTGGATCGTGCCC CCCATGTCCGGAAGCATCACCCCTGTGTTCCACCAGGAGATGCTCAACTACCGGCTCACCCCCTCCTTCG AATACCAGCCTGATCCCTGGAACACGCATGTCTGGAAAGGCACCAACGGGACCCCCACAAAGCGGCGAGC CATTGGCTTCAAGAAGCTAGCAGAAGCTGTCAAGTTCTCGGCCAAGCTGATGGGGCAGGCTATGGCCAAG AGGGTGAAAGCGACCATCCTCTATGCCACAGAGACAGGCAAATCGCAAGCTTATGCCAAGACCTTGTGTG AGATCTTCAAACACGCCTTTGATGCCAAGGTGATGTCCATGGAAGAATATGACATTGTGCACCTGGAACA TGAAACTCTGGTCCTTGTGGTCACCAGCACCTTTGGCAATGGAGATCCCCCTGAGAATGGGGAGAAATTC GGCTGTGCTTTGATGGAAATGAGGCACCCCAACTCTGTGCAGGAAGAAAGGAAGAGCTACAAGGTCCGAT TCAACAGCGTCTCCTCCTACTCTGACTCCCAAAAATCATCAGGCGATGGGCCCGACCTCAGAGACAACTT TGAGAGTGCTGGACCCCTGGCCAATGTGAGGTTCTCAGTTTTTGGCCTCGGCTCACGAGCATACCCTCAC TTTTGCGCCTTCGGACACGCTGTGGACACCCTCCTGGAAGAACTGGGAGGGGAGAGGATCCTGAAGATGA GGGAAGGGGATGAGCTCTGTGGGCAGGAAGAGGCTTTCAGGACCTGGGCCAAGAAGGTCTTCAAGGCAGC CTGTGATGTCTTCTGTGTGGGAGATGATGTCAACATTGAAAAGGCCAACAATTCCCTCATCAGCAATGAT CGCAGCTGGAAGAGAAACAAGTTCCGCCTCACCTTTGTGGCCGAAGCTCCAGAACTCACACAAGGTCTAT CCAATGTCCACAAAAAGCGAGTCTCAGCTGCCCGGCTCCTTAGCCGTCAAAACCTCCAGAGCCCTAAATC CAGTCGGTCAACTATCTTCGTGCGTCTCCACACCAACGGGAGCCAGGAGCTGCAGTACCAGCCTGGGGAC CACCTGGGTGTCTTCCCTGGCAACCACGAGGACCTCGTGAATGCCCTGATCGAGCGGCTGGAGGACGCGC CGCCTGTCAACCAGATGGTGAAAGTGGAACTGCTGGAGGAGCGGAACACGGCTTTAGGTGTCATCAGTAA CTGGACAGACGAGCTCCGCCTCCCGCCCTGCACCATCTTCCAGGCCTTCAAGTACTACCTGGACATCACC ACGCCACCAACGCCTCTGCAGCTGCAGCAGTTTGCCTCCCTAGCTACCAGCGAGAAGGAGAAGCAGCGTC TGCTGGTCCTCAGCAAGGGTTTGCAGGAGTACGAGGAATGGAAATGGGGCAAGAACCCCACCATCGTGGA GGTGCTGGAGGAGTTCCCATCTATCCAGATGCCGGCCACCCTGCTCCTGACCCAGCTGTCCCTGCTGCAG CCCCGCTACTATTCCATCAGCTCCTCCCCAGACATGTACCCTGATGAAGTGCACCTCACTGTGGCCATCG TTTCCTACCGCACTCGAGATGGAGAAGGACCAATTCACCACGGCGTATGCTCCTCCTGGCTCAACCGGAT ACAGGCTGACGAACTGGTCCCCTGTTTCGTGAGAGGAGCACCCAGCTTCCACCTGCCCCGGAACCCCCAA GTCCCCTGCATCCTCGTTGGACCAGGCACCGGCATTGCCCCTTTCCGAAGCTTCTGGCAACAGCGGCAAT TTGATATCCAACACAAAGGAATGAACCCCTGCCCCATGGTCCTGGTCTTCGGGTGCCGGCAATCCAAGAT AGATCATATCTACAGGGAAGAGACCCTGCAGGCCAAGAACAAGGGGGTCTTCAGAGAGCTGTACACGGCT TACTCCCGGGAGCCAGACAAACCAAAGAAGTACGTGCAGGACATCCTGCAGGAGCAGCTGGCGGAGTCTG TGTACCGAGCCCTGAAGGAGCAAGGGGGCCACATATACGTCTGTGGGGACGTCACCATGGCTGCTGATGT CCTCAAAGCCATCCAGCGCATCATGACCCAGCAGGGGAAGCTCTCGGCAGAGGACGCCGGCGTATTCATC AGCCGGATGAGGGATGACAACCGATACCATGAGGATATTTTTGGAGTCACCCTGCGAACGTACGAAGTGA CCAACCGCCTTAGATCTGAGTCCATTGCCTTCATTGAAGAGAGCAAAAAAGACACCGATGAGGTTTTCAG CTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233200 representing NM_001204214 Red=Cloning site Green=Tags(s) MGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTEL IYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHD FRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELV LEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVA KKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVP PMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAK RVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKF GCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPH FCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISND RSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGD HLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDIT TPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQ PRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQ VPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTA YSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFI SRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001204214
ORF Size	3294 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204214.1, NP_001191143.1
RefSeq Size	10776 bp
RefSeq ORF	3297 bp
Locus ID	4842
UniProt ID	P29475
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer
MW	125.6 kDa
Gene Summary	The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

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