UBE4A (NM_001204077) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	E4; UBOX2; UFD2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233197 representing NM_001204077 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCTGGCTG ATGCCAAACAGTTTGCGGCAATCCAAAAAGAGCAGCTGAAGCAACAATCTGATGAACTCCCAGCTAGCCC AGATGACTCGGATAATAGCGTGTCAGAGAGCCTGGATGAATTCGATTACTCTGTGGCTGAGATTAGCCGC TCATTCCGATCACAGCAGGAAATATGTGAGCAACTCAACATCAATCACATGATCCAAAGGATCTTCCTTA TTACTCTGGACAACAGTGATCCCAGCTTGAAAAGCGGGAATGGCATCCCTAGCCGTTGTGTGTATTTGGA AGAAATGGCAGTAGAGCTAGAAGATCAAGACTGGCTTGATATGAGCAATGTTGAGCAGGCCCTCTTCGCT CGCTTATTACTTCAAGATCCAGGCAACCACTTAATTAACATGACTTCTTCTACAACGCTAAATCTCTCTG CTGATCGAGATGCAGGAGAGAGGCACATTTTTTGTTACCTTTACTCCTGCTTCCAGAGAGCCAAGGAAGA GATTACCAAAGTTCCAGAGAACCTGCTACCCTTTGCAGTGCAGTGCAGAAACCTCACTGTGTCCAATACC CGAACAGTTCTTCTCACCCCAGAGATCTATGTTGACCAAAACATCCATGAGCAACTGGTAGATTTGATGT TAGAAGCCATCCAGGGAGCCCATTTTGAAGATGTAACTGAGTTTCTGGAAGAGGTCATTGAAGCCTTGAT ATTGGATGAGGAAGTTAGAACATTTCCAGAAGTCATGATTCCAGTGTTTGATATTTTATTGGGCCGAATA AAAGATCTAGAGCTCTGTCAGATCCTTTTGTATGCATATCTGGATATTCTTCTCTATTTCACTAGGCAAA AAGATATGGCAAAGGTTTTTGTAGAATACATTCAGCCCAAGGACCCTACCAATGGGCAAATGTACCAGAA GACCTTGCTGGGAGTAATTCTGAGTATCTCCTGCTTATTAAAGACTCCGGGTGTTGTAGAAAATCATGGC TACTTTTTGAATCCATCTCGTTCCAGCCCCCAGGAGATCAAAGTACAGGAGGCCAACATCCATCAGTTCA TGGCTCAGTTCCACGAAAAGATCTACCAGATGCTGAAGAACTTACTCCAGCTCTCTCCAGAAACCAAACA CTGTATCTTGTCCTGGCTTGGAAACTGTTTGCATGCAAATGCAGGCCGCACCAAGATTTGGGCCAATCAG ATGCCAGAAATCTTTTTCCAAATGTATGCCTCAGATGCTTTCTTTCTGAATCTGGGTGCTGCTCTCCTGA AGCTATGCCAGCCATTTTGCAAACCCAGATCCTCTCGGCTCCTCACCTTTAATCCCACATACTGTGCCCT CAAGGAGTTGAATGATGAAGAACGAAAAATTAAAAATGTACACATGAGAGGTTTGGACAAAGAAACCTGT TTGATCCCAGCTGTGCAGGAGCCGAAGTTTCCACAGAACTACAACCTTGTAACAGAGAACCTTGCTCTGA CAGAGTACACCTTGTACTTGGGATTTCACAGGTTGCATGATCAGATGGTAAAAATCAACCAAAATCTGCA TCGGCTGCAGGTTGCCTGGCGGGATGCTCAGCAAAGTTCTAGCCCTGCTGCTGACAATCTTCGTGAGCAG TTTGAACGACTGATGACCATCTATCTTTCTACCAAGACTGCCATGACAGAGCCACAAATGCTACAAAACT GCCTAAACTTGCAGGTGTCCATGGCTGTTCTACTGGTTCAACTGGCCATAGGCAATGAGGGCTCACAGCC AATAGAGCTAACCTTTCCTTTGCCAGATGGCTACAGCTCTTTGGCTTATGTGCCAGAATTTTTTGCAGAT AACCTGGGTGATTTTCTCATTTTTCTCCGCCGCTTTGCCGATGACATTTTGGAGACATCAGCAGATTCCC TGGAGCATGTCCTTCACTTTATCACCATTTTCACTGGAAGCATAGAAAGAATGAAGAATCCCCACCTGAG GGCCAAACTAGCAGAGGTGTTGGAAGCAGTGATGCCCCACCTGGATCAGACCCCAAATCCCTTGGTATCC AGTGTGTTCCACCGGAAACGTGTGTTCTGCAACTTTCAGTATGCACCCCAACTTGCAGAGGCTCTAATCA AGGTTTTTGTGGACATCGAATTTACAGGAGACCCCCATCAATTTGAACAGAAGTTTAATTACCGCCGTCC CATGTATCCTATCCTAAGATACATGTGGGGGACAGATACCTATCGGGAGAGCATTAAGGATTTGGCTGAC TATGCCTCTAAGAATTTAGAAGCCATGAATCCCCCACTTTTCCTCCGCTTTCTTAACCTGCTAATGAATG ATGCCATCTTCCTTTTGGATGAAGCCATACAGTATTTGAGCAAGATAAAGATTCAGCAAATTGAGAAGGA TCGAGGTGAATGGGATAGTCTGACTCCAGAAGCCCGCCGAGAAAAGGAGGCTGGCCTACAGATGTTTGGA CAGCTGGCACGTTTCCATAACATCATGTCCAATGAAACAATCGGTACCCTTGCCTTTCTCACATCAGAGA TCAAGTCACTCTTTGTGCATCCCTTCCTGGCTGAGCGCATCATCTCCATGTTGAACTACTTCCTGCAACA CCTGGTTGGCCCCAAGATGGGTGCCTTAAAAGTCAAGGACTTCAGCGAATTTGACTTCAAACCCCAGCAG CTTGTATCAGATATCTGCACTATCTACTTAAATCTTGGGGATGAGGAGAATTTCTGTGCCACTGTGCCCA AGGATGGACGTTCCTATTCCCCAACTCTCTTTGCACAGACAGTTCGAGTCTTGAAGAAAATAAATAAGCC TGGGAATATGATTATGGCTTTCAGCAACTTGGCAGAGAGAATCAAGTCTCTTGCAGACCTCCAACAACAG GAAGAGGAAACCTATGCAGATGCCTGTGATGAGTTCCTGGATCCCATTATGAGCACACTGATGTGTGACC CTGTGGTGCTGCCATCTTCCAGAGTCACTGTGGATAGATCCACCATTGCAAGACATTTGCTCAGTGACCA AACAGATCCCTTTAACCGTAGTCCCCTCACCATGGACCAGATCCGGCCAAACACAGAACTAAAAGAAAAA ATCCAACGGTGGCTTGCAGAGAGGAAACAACAAAAGGAGCAACTTGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233197 representing NM_001204077 Red=Cloning site Green=Tags(s) MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVAEISR SFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQALFA RLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNT RTVLLTPEIYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALILDEEVRTFPEVMIPVFDILLGRI KDLELCQILLYAYLDILLYFTRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTPGVVENHG YFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGRTKIWANQ MPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETC LIPAVQEPKFPQNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQ FERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAYVPEFFAD NLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVS SVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDTYRESIKDLAD YASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFG QLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQ LVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIMAFSNLAERIKSLADLQQQ EEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEK IQRWLAERKQQKEQLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001204077
ORF Size	3198 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204077.2
RefSeq Size	6123 bp
RefSeq ORF	3201 bp
Locus ID	9354
UniProt ID	Q14139
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
MW	123 kDa
Gene Summary	This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011]

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