GLT28D1 (ALG13) (NM_001257234) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CDG1S; CXorf45; DEE36; EIEE36; GLT28D1; MDS031; TDRD13; YGL047W
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233187 representing NM_001257234 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAATCATCAGCTGGAACTGGCAAAGCAGCTACACAAAGAGGGTCATCTCTTCTATTGTACCTGCA GGGTCCTGACTTGTCCTGGGCAAGCCAAGTCCATTGCTTCTGCTCCTGGGAAGTGCCAAGATTCTGCAGC GCTGACTTCAACTGCCTTTTCAGGCCTAGACTTTGGGCTGCTTTCCGGATACCTGCATAAGCAAGCCCTT GTTACTGCTACCCATCCTACCTGCACCCTGCTTTTTCCCTCTTGCCACGCTTTTTTTCCTCTCCCTCTTA CCCCCACCCTGTACAAAATGCATAAAGGATGGAAAAACTACTGCAGCCAGAAGTCTTTGAATGAGGCATC AATGGATGAATATTTAGGCAGCTTAGGGCTGTTTCGAAAGCTGACTGCCAAGGATGCCTCTTGCCTCTTT CGGGCCATTTCGGAGCAGTTGTTTTGCAGCCAGGTCCATCATTTGGAAATCAGGAAGGCTTGTGTCTCAT ATATGAGGGAAAATCAACAAACTTTTGAGTCTTATGTGGAGGGATCTTTTGAGAAATACCTGGAACGGTT GGGAGATCCCAAGGAAAGTGCTGGCCAGCTGGAAATAAGAGCTCTTTCTCTAATTTATAATCGGGATTTC ATTCTTTATCGCTTTCCTGGAAAACCTCCAACTTATGTCACAGATAATGGCTATGAAGACAAGATTCTAC TCTGCTACTCAAGTAGTGGTCACTATGATTCTGTGTACTCAAAACAATTTCAGTCAAGTGCAGCTGTTTG TCAGGCTGTATTGTACGAAATTCTCTATAAAGATGTGTTTGTTGTGGATGAAGAAGAGTTGAAGACTGCG ATTAAATTGTTTCGAAGTGGTTCTAAGAAGAACAGAAATAATGCTGTAACTGGAAGCGAGGATGCCCATA CTGATTACAAGAGTTCAAATCAGAATAGGATGGAAGAGTGGGGTGCCTGCTACAATGCTGAAAATATACC AGAGGGCTACAATAAAGGAACAGAAGAAACAAAGTCTCCAGAAAATCCATCAAAGATGCCCTTCCCCTAT AAGGTGCTCAAAGCCCTGGATCCAGAAATCTATCGTAATGTAGAATTTGATGTTTGGTTGGACAGCAGAA AAGAACTTCAAAAATCTGATTACATGGAGTATGCTGGGAGACAGTACTATTTGGGAGACAAGTGTCAGGT TTGCTTGGAATCAGAAGGAAGATATTATAATGCTCATATCCAGGAAGTTGGAAATGAGAACAATTCAGTA ACAGTCTTCATTGAAGAATTGGCGGAAAAGCATGTTGTTCCACTGGCTAACTTAAAACCAGTTACCCAAG TGATGTCTGTTCCTGCCTGGAATGCTATGCCCAGTCGGAAAGGAAGAGGTTACCAGAAAATGCCTGGGGG TTATGTCCCGGAAATAGTTATATCAGAGATGGACATAAAGCAACAGAAGAAGATGTTCAAGAAAATTCGA GGGAAAGAAGTTTACATGACTATGGCTTACGGCAAGGGAGACCCCCTCCTCCCACCCAGGCTGCAGCACA GTATGCATTATGGGCACGATCCTCCAATGCACTACTCACAGACAGCTGGCAATGTTATGTCTAATGAACA TTTTCATCCTCAGCATCCATCTCCGAGACAAGGTCGGGGATATGGGATGCCCAGGAATTCATCTCGGTTT ATAAACAGGCACAACATGCCGGGCCCTAAAGTTGATTTTTACCCAGGCCCAGGTAAAAGGTGCTGCCAGA GCTATGATAACTTCTCTTATAGATCTCGTTCATTTAGACGTAGTCACCGCCAGATGAGTTGTGTGAATAA GGAGTCCCAGTATGGATTTACCCCAGGGAATGGACAGATGCCCAGGGGCTTGGAAGAAACTATTACTTTT TATGAAGTTGAAGAAGGGGATGAGACTGCTTATCCAACTTTACCTAATCATGGAGGTCCCTCTACAATGG TTCCTGCTACTTCAGGATACTGTGTTGGAAGGCGGGGACATAGCTCAGGCAAACAGACTTTGAATTTAGA GGAGGGCAATGGCCAGAGTGAAAATGGGCGATATCATGAAGAATATCTTTATCGTGCAGAGCCAGACTAT GAAACTTCAGGTGTTTATAGCACAACTGCATCTACAGCAAACTTGTCTCTTCAGGACAGAAAGTCATGTT CTATGTCTCCTCAGGACACAGTTACCTCATACAACTACCCCCAGAAGATGATGGGAAATATTGCAGCAGT TGCAGCTTCCTGTGCCAATAATGTTCCAGCTCCAGTCTTATCTAACGGTGCAGCGGCTAATCAAGCTATT AGTACCACTTCAGTTTCCTCACAGAATGCTATACAGCCTCTCTTTGTATCTCCACCTACACACGGCAGGC CAGATACAAAAGTTTTGCAGTACTATTTCAATCTAGGATTGCAGTGCTATTACCACAGCTACTGGCACTC CATGGTCTATGTGCCACAGATGCAGCAGCAGCTTCATGTAGAGAATTATCCAGTCTATACTGAGCCACCT CTGGTAGATCAAACCGTTCCTCAATGCTACAGTGAGGTGAGGAGAGAAGATGGCATACAGGCGGAAGCAT CAGCAAATGATACTTTTCCGAATGCTGATTCTTCATCTGTCCCTCATGGAGCAGTCTATTATCCAGTAAT GTCAGATCCCTATGGGCAGCCACCTTTGCCAGGTTTTGACTCCTGCCTTCCGGTTGTGCCAGATTATTCC TGTGTTCCCCCCTGGCATCCAGTTGGTACAGCATATGGTGGTTCTTCTCAAATTCATGGTGCTATAAATC CTGGGCCAATTGGCTGTATTGCTCCATCTCCCCCAGCTTCTCATTATGTACCTCAGGGTATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233187 representing NM_001257234 Red=Cloning site Green=Tags(s) MNNHQLELAKQLHKEGHLFYCTCRVLTCPGQAKSIASAPGKCQDSAALTSTAFSGLDFGLLSGYLHKQAL VTATHPTCTLLFPSCHAFFPLPLTPTLYKMHKGWKNYCSQKSLNEASMDEYLGSLGLFRKLTAKDASCLF RAISEQLFCSQVHHLEIRKACVSYMRENQQTFESYVEGSFEKYLERLGDPKESAGQLEIRALSLIYNRDF ILYRFPGKPPTYVTDNGYEDKILLCYSSSGHYDSVYSKQFQSSAAVCQAVLYEILYKDVFVVDEEELKTA IKLFRSGSKKNRNNAVTGSEDAHTDYKSSNQNRMEEWGACYNAENIPEGYNKGTEETKSPENPSKMPFPY KVLKALDPEIYRNVEFDVWLDSRKELQKSDYMEYAGRQYYLGDKCQVCLESEGRYYNAHIQEVGNENNSV TVFIEELAEKHVVPLANLKPVTQVMSVPAWNAMPSRKGRGYQKMPGGYVPEIVISEMDIKQQKKMFKKIR GKEVYMTMAYGKGDPLLPPRLQHSMHYGHDPPMHYSQTAGNVMSNEHFHPQHPSPRQGRGYGMPRNSSRF INRHNMPGPKVDFYPGPGKRCCQSYDNFSYRSRSFRRSHRQMSCVNKESQYGFTPGNGQMPRGLEETITF YEVEEGDETAYPTLPNHGGPSTMVPATSGYCVGRRGHSSGKQTLNLEEGNGQSENGRYHEEYLYRAEPDY ETSGVYSTTASTANLSLQDRKSCSMSPQDTVTSYNYPQKMMGNIAAVAASCANNVPAPVLSNGAAANQAI STTSVSSQNAIQPLFVSPPTHGRPDTKVLQYYFNLGLQCYYHSYWHSMVYVPQMQQQLHVENYPVYTEPP LVDQTVPQCYSEVRREDGIQAEASANDTFPNADSSSVPHGAVYYPVMSDPYGQPPLPGFDSCLPVVPDYS CVPPWHPVGTAYGGSSQIHGAINPGPIGCIAPSPPASHYVPQGM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001257234
ORF Size	2862 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001257234.1, NP_001244163.1
RefSeq Size	3911 bp
RefSeq ORF	2865 bp
Locus ID	79868
UniProt ID	Q9NP73
Protein Pathways	Metabolic pathways, N-Glycan biosynthesis
MW	106.9 kDa
Gene Summary	The protein encoded by this gene is a subunit of a bipartite UDP-N-acetylglucosamine transferase. It heterodimerizes with asparagine-linked glycosylation 14 homolog to form a functional UDP-GlcNAc glycosyltransferase that catalyzes the second sugar addition of the highly conserved oligosaccharide precursor in endoplasmic reticulum N-linked glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]

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