HERC3 (NM_001271602) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233180 representing NM_001271602 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTACTGAGGATTCTGTGGCAGTGCCCAGGTTAATACAAAAGCTGAACCAGCAAACAATATTACAAG TTTCCTGTGGCAACTGGCATTGCTTGGCTCTTGCGGCTGATGGCCAGTTCTTCACCTGGGGAAAGAACAG CCATGGGCAGCTTGGCTTAGGGAAGGAGTTCCCCTCCCAAGCCAGCCCACAGAGGGTGAGGTCCCTGGAG GGGATCCCACTGGCTCAGGTGGCTGCCGGAGGGGCTCACAGCTTTGCCCTGTCTCTCTCAGGAGCTGTTT TTGGCTGGGGGATGAATAATGCCGGGCAGCTAGGGCTCAGTGATGAAAAAGATCGAGAATCTCCATGCCA TGTAAAACTCTTACGCACGCAAAAAGTTGTCTATATTAGTTGTGGAGAAGAACACACAGCAGTTCTCACA AAGAGTGGAGGTGTGTTTACCTTTGGCGCTGGTTCCTGTGGGCAACTTGGACACGACTCCATGAATGATG AGGTTAACCCTAGAAGAGTTCTAGAGCTGATGGGTAGTGAAGTAACTCAAATTGCTTGTGGCAGACAACA TACCCTAGCCTTCGTGCCTTCTTCTGGACTCATCTATGCATTTGGTTGTGGAGCAAGAGGTCAATTAGGA ACTGGGCACACTTGTAATGTTAAGTGCCCATCTCCTGTCAAGGGTTACTGGGCTGCCCACAGTGGCCAGC TTTCAGCCCGAGCTGATCGCTTTAAATATCATATCGTTAAGCAGATCTTCTCTGGAGGAGACCAGACTTT TGTACTTTGCTCCAAATACGAGAATTATTCTCCTGCTGTTGACTTCAGGACTATGAACCAAGCACATTAT ACCAGTTTAATAAATGATGAAACCATAGCAGTTTGGAGACAAAAACTCTCAGAACACAACAATGCAAATA CAATCAATGGTGTTGTTCAGATATTATCTTCTGCAGCCTGTTGGAATGGAAGTTTTCTTGAAAAAAAAAT TGATGAACATTTTAAAACGAGTCCCAAAATCCCTGGGATTGACCTGAACTCAACTAGGGTGTTATTTGAG AAGTTAATGAACTCTCAGCACTCCATGATTCTAGAACAGATTTTGAACAGTTTTGAAAGTTGTCTGATTC CCCAGTTGTCAAGCTCACCACCAGATGTTGAAGCCATGAGAATCTATTTAATACTACCTGAGTTTCCCCT ACTCCAGGATTCCAAGTATTATATAACATTGACTATTCCCTTGGCTATGGCCATTCTTCGGCTGGATACA AACCCCAGCAAAGTACTAGATAACTGGTGGTCTCAGGTATGCCCGAAATATTTCATGAAGCTGGTAAACC TCTATAAAGGTGCAGTCCTTTATCTACTGAGGGGAAGAAAGACATTCTTAATTCCCGTACTGTTTAACAA TTATATCACAGCAGCTCTCAAACTCTTGGAGAAGTTATATAAGGTAAATCTTAAAGTGAAGCATGTGGAA TATGATACATTTTACATTCCTGAGATTTCCAATCTCGTGGACATTCAGGAAGACTACCTCATGTGGTTCT TGCATCAAGCAGGGATGAAGGCTAGACCATCAATAATACAGGATACTGTAACACTTTGTTCCTACCCTTT CATCTTTGATGCCCAAGCCAAGACCAAAATGTTACAGACAGATGCTGAACTACAGATGCAGGTGGCAGTC AATGGAGCCAACCTGCAGAATGTCTTCATGCTTCTCACCCTGGAGCCTCTGCTGGCCAGAAGCCCCTTCC TGGTCCTTCACGTTCGCAGGAACAACCTTGTTGGAGATGCCCTAAGAGAGCTGAGCATTCATTCTGATAT TGATTTGAAAAAGCCTCTCAAAGTAATCTTTGATGGTGAAGAAGCAGTGGATGCCGGTGGTGTTACAAAG GAATTTTTTCTTTTGCTGTTAAAAGAACTTTTGAATCCCATCTATGGAATGTTTACCTACTATCAAGATT CAAATCTCTTGTGGTTTTCAGACACGTGTTTTGTAGAGCACAACTGGTTTCACTTGATTGGTATAACCTG TGGACTAGCTATCTACAACTCCACTGTGGTCGATCTCCACTTCCCATTGGCTCTCTACAAGAAGTTACTC AATGTAAAGCCTGGCTTGGAAGACTTAAAGGAGTTGTCACCCACTGAAGGAAGGAGTCTCCAAGAGCTTT TAGATTACCCCGGGGAGGATGTGGAGGAGACTTTCTGCCTCAACTTCACGATCTGCCGAGAAAGCTATGG AGTGATTGAACAGAAGAAGCTGATACCTGGGGGAGATAATGTAACTGTGTGCAAGGATAACAGGCAGGAA TTTGTGGATGCTTATGTGAATTATGTCTTCCAAATCTCAGTTCATGAATGGTACACAGCCTTCTCTAGTG GCTTCCTAAAGGTGTGTGGTGGCAAAGTACTTGAGCTCTTCCAGCCTTCAGAACTGAGGGCTATGATGGT GGGGAACAGCAACTACAACTGGGAAGAACTGGAAGAGACTGCCATCTACAAGGGAGATTACTCGGCCACA CATCCCACTGTAAAACTATTTTGGGAAACATTTCATGAGTTTCCATTGGAAAAGAAGAAGAAGTTTCTCT TGTTCCTGACAGGCAGCGATCGGATTCCCATCTACGGCATGGCCAGTCTGCAGATTGTCATCCAGTCCAC AGCCAGCGGGGAGGAGTACTTGCCGGTGGCCCACACTTGCTACAACCTTCTTGACCTCCCCAAGTACAGC AGCAAAGAGATTCTGAGTGCCCGGCTGACCCAGGCCCTTGACAACTATGAAGGGTTTAGTTTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233180 representing NM_001271602 Red=Cloning site Green=Tags(s) MTTEDSVAVPRLIQKLNQQTILQVSCGNWHCLALAADGQFFTWGKNSHGQLGLGKEFPSQASPQRVRSLE GIPLAQVAAGGAHSFALSLSGAVFGWGMNNAGQLGLSDEKDRESPCHVKLLRTQKVVYISCGEEHTAVLT KSGGVFTFGAGSCGQLGHDSMNDEVNPRRVLELMGSEVTQIACGRQHTLAFVPSSGLIYAFGCGARGQLG TGHTCNVKCPSPVKGYWAAHSGQLSARADRFKYHIVKQIFSGGDQTFVLCSKYENYSPAVDFRTMNQAHY TSLINDETIAVWRQKLSEHNNANTINGVVQILSSAACWNGSFLEKKIDEHFKTSPKIPGIDLNSTRVLFE KLMNSQHSMILEQILNSFESCLIPQLSSSPPDVEAMRIYLILPEFPLLQDSKYYITLTIPLAMAILRLDT NPSKVLDNWWSQVCPKYFMKLVNLYKGAVLYLLRGRKTFLIPVLFNNYITAALKLLEKLYKVNLKVKHVE YDTFYIPEISNLVDIQEDYLMWFLHQAGMKARPSIIQDTVTLCSYPFIFDAQAKTKMLQTDAELQMQVAV NGANLQNVFMLLTLEPLLARSPFLVLHVRRNNLVGDALRELSIHSDIDLKKPLKVIFDGEEAVDAGGVTK EFFLLLLKELLNPIYGMFTYYQDSNLLWFSDTCFVEHNWFHLIGITCGLAIYNSTVVDLHFPLALYKKLL NVKPGLEDLKELSPTEGRSLQELLDYPGEDVEETFCLNFTICRESYGVIEQKKLIPGGDNVTVCKDNRQE FVDAYVNYVFQISVHEWYTAFSSGFLKVCGGKVLELFQPSELRAMMVGNSNYNWEELEETAIYKGDYSAT HPTVKLFWETFHEFPLEKKKKFLLFLTGSDRIPIYGMASLQIVIQSTASGEEYLPVAHTCYNLLDLPKYS SKEILSARLTQALDNYEGFSLA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271602
ORF Size	2796 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271602.2
RefSeq Size	4730 bp
RefSeq ORF	2799 bp
Locus ID	8916
UniProt ID	Q15034
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
MW	105.1 kDa
Gene Summary	This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012]

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