ALDH1L1 (NM_001270365) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	10-fTHF; 10-FTHFDH; FDH; FTHFD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233141 representing NM_001270365 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATTGCAGTGATTGGACAGAGCCTGTTTGGCCAGGAAGTTTACTGCCACCTGAGGAAGGAGGGCC ACGAAGTGGTGGGTGTGTTCACTGTTCCAGACAAGGATGGAAAGGCCGACCCCCTGGGTCTGGAAGCTGA GAAGGATGGAGTGCCGGTATTCAAGTACTCCCGGTGGCGTGCAAAAGGACAGGCTTTGCCTGATGTGGTG GCAAAATACCAGGCTTTGGGGGCCGAGCTCAACGTCCTGCCCTTCTGCAGCCAATTCATCCCCATGGAGA TAATCAGTGCCCCCCGGCATGGCTCCATCATCTATCACCCGTCACTGCTCCCTAGGCACCGAGGGGCCTC GGCCATTCACAACTGGATCCGCGGGAACGACAAGGTGCCGGGAGCCTGGACAGAGGCCTGTGAACAGAAA CTGACATTTTTCAACTCAACGCTGAACACTTCAGGCCTGGTGCCCGAGGGAGACGCTTTGCCCATCCCAG GAGCCCATCGGCCAGGGGTGGTCACCAAAGCAGGACTCATCCTCTTTGGGAATGATGACAAAATGCTGCT GGTGAAGAATATTCAGCTGGAGGATGGCAAAATGATCCTGGCCTCGAACTTCTTTAAGGGGGCAGCCAGC AGTGTCCTTGAGCTGACAGAGGCAGAGCTGGTTACTGCGGAGGCTGTGCGGAGTGTTTGGCAGCGGATCC TCCCCAAAGTCCTGGAGGTTGAAGACTCCACTGATTTCTTCAAGTCAGGGGCCGCGTCTGTGGACGTTGT GAGGCTGGTGGAGGAAGTGAAGGAGCTGTGTGATGGCCTGGAGTTAGAAAATGAAGATGTGTACATGGCA TCCACCTTTGGGGACTTCATCCAGCTGTTAGTGAGGAAGCTGCGAGGGGACGATGAGGAGGGCGAGTGCA GCATTGACTACGTGGAAATGGCAGTGAACAAGCGCACTGTCCGCATGCCCCACCAGCTCTTCATTGGGGG GGAGTTCGTGGATGCCGAGGGCGCCAAGACCTCTGAGACCATCAATCCCACCGATGGAAGTGTCATCTGC CAGGTATCCCTGGCCCAAGTCACCGACGTCGACAAGGCAGTGGCCGCAGCCAAGGATGCCTTTGAGAATG GACGGTGGGGGAAGATCAGTGCGCGGGACCGGGGCCGGCTGATGTACAGGTTGGCAGATCTCATGGAGCA GCACCAGGAGGAGCTGGCCACCATTGAGGCCCTGGATGCGGGTGCCGTCTACACGCTGGCCCTGAAGACC CACGTGGGCATGTCCATCCAGACCTTCCGCTACTTTGCTGGCTGGTGTGACAAGATCCAGGGCTCCACCA TCCCCATCAACCAGGCCAGACCCAACCGCAACCTGACCTTGACCAGGAAGGAGCCTGTTGGGGTTTGTGG CATCATCATCCCCTGGAACTATCCCCTGATGATGCTGTCCTGGAAGACAGCTGCCTGCCTGGCTGCCGGG AACACAGTGGTGATCAAGCCTGCTCAGGTGACCCCACTCACAGCCTTGAAGTTTGCAGAGCTGACATTAA AGGCCGGCATTCCCAAAGGTGTGGTTAACGTCCTCCCAGGATCTGGCTCCCTGGTCGGCCAGAGACTCTC AGACCATCCTGATGTGAGGAAAATCGGGTTCACAGGCTCCACAGAGGTGGGCAAGCACATCATGAAAAGC TGTGCCATAAGTAACGTGAAGAAGGTGTCCCTGGAACTGGGCGGGAAGTCACCCCTCATCATCTTTGCTG ACTGTGACCTCAACAAGGCTGTGCAGATGGGGATGAGTTCTGTTTTCTTCAACAAAGGAGAGAATTGCAT TGCAGCAGGCCGACTCTTTGTGGAGGACTCCATTCATGATGAGTTCGTGCGGAGAGTGGTAGAAGAGGTG CGGAAGATGAAGGTGGGCAACCCGCTGGACAGGGACACCGACCACGGGCCGCAGAATCACCATGCCCACC TTGTGAAGCTGATGGAGTACTGCCAGCATGGCGTGAAGGAAGGGGCCACACTGGTCTGCGGCGGGAATCA GGTCCCTCGGCCAGGGTTCTTCTTTGAGCCAACTGTTTTCACAGACGTGGAAGACCACATGTTCATAGCC AAGGAGGAGTCCTTCGGGCCTGTCATGATCATCTCTCGGTTTGCTGATGGGGACTTGGATGCCGTGCTGT CTCGGGCCAATGCCACGGAATTTGGCCTGGCTTCTGGTGTCTTCACCAGGGACATCAACAAGGCCCTGTA TGTCAGTGACAAGCTCCAGGCAGGCACTGTGTTTGTCAACACGTACAACAAGACCGACGTGGCCGCTCCC TTCGGAGGATTCAAACAGTCTGGATTTGGCAAAGATCTAGGAGAGGCGGCTCTGAACGAGTACCTGCGGG TCAAGACAGTGACCTTCGAATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233141 representing NM_001270365 Red=Cloning site Green=Tags(s) MKIAVIGQSLFGQEVYCHLRKEGHEVVGVFTVPDKDGKADPLGLEAEKDGVPVFKYSRWRAKGQALPDVV AKYQALGAELNVLPFCSQFIPMEIISAPRHGSIIYHPSLLPRHRGASAIHNWIRGNDKVPGAWTEACEQK LTFFNSTLNTSGLVPEGDALPIPGAHRPGVVTKAGLILFGNDDKMLLVKNIQLEDGKMILASNFFKGAAS SVLELTEAELVTAEAVRSVWQRILPKVLEVEDSTDFFKSGAASVDVVRLVEEVKELCDGLELENEDVYMA STFGDFIQLLVRKLRGDDEEGECSIDYVEMAVNKRTVRMPHQLFIGGEFVDAEGAKTSETINPTDGSVIC QVSLAQVTDVDKAVAAAKDAFENGRWGKISARDRGRLMYRLADLMEQHQEELATIEALDAGAVYTLALKT HVGMSIQTFRYFAGWCDKIQGSTIPINQARPNRNLTLTRKEPVGVCGIIIPWNYPLMMLSWKTAACLAAG NTVVIKPAQVTPLTALKFAELTLKAGIPKGVVNVLPGSGSLVGQRLSDHPDVRKIGFTGSTEVGKHIMKS CAISNVKKVSLELGGKSPLIIFADCDLNKAVQMGMSSVFFNKGENCIAAGRLFVEDSIHDEFVRRVVEEV RKMKVGNPLDRDTDHGPQNHHAHLVKLMEYCQHGVKEGATLVCGGNQVPRPGFFFEPTVFTDVEDHMFIA KEESFGPVMIISRFADGDLDAVLSRANATEFGLASGVFTRDINKALYVSDKLQAGTVFVNTYNKTDVAAP FGGFKQSGFGKDLGEAALNEYLRVKTVTFEY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270365
ORF Size	2403 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270365.2
RefSeq Size	3003 bp
RefSeq ORF	2406 bp
Locus ID	10840
UniProt ID	O75891
Protein Families	Druggable Genome
Protein Pathways	One carbon pool by folate
MW	88.1 kDa
Gene Summary	The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]

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