Neuroligin 4 (NLGN4Y) (NM_001206850) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HNL4Y
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233056 representing NM_001206850 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCTATATCCATGGGGGATCTTACATGGAGGGAACCGGTAACATGATTGATGGCAGCATTTTGGCCA GCTATGGGAACGTCATCGTTATCACCATTAACTACCGTCTGGGAATACTAGGGTTTTTAAGTACCGGTGA CCAGGCAGCAAAAGGCAACTATGGGCTCCTGGATCAGATTCAAGCACTGAGGTGGATTGAGGAGAATGTC GGAGCCTTTGGCGGGGACCCCAAGAGAGTGACTATCTTTGGCTCGGGGGCTGGGGCCTCCTGTGTCAGCC TGTTGACCCTGTCCCACTACTCAGAAGGTCTCTTCCAGAAGGCCATCATTCAGAGCGGCACTGCCCTGTC CAGCTGGGCAGTGAACTACCAGCCGGCCAAGTACACTCGGATATTGGCAGACAAGGTCGGCTGCAACATG CTGGACACCACGGACATGGTAGAATGTCTGAAGAACAAGAACTACAAGGAGCTCATCCAGCAGACCATCA CCCCGGCCACCTACCACATAGCCTTTGGGCCGGTGATCGACGGCGACGTCATCCCAGACGACCCCCAGAT CCTGATGGAGCAAGGCGAGTTCCTCAACTACGACATCATGCTGGGCGTCAACCAAGGGGAAGGCCTGAAG TTCGTGGACGGCATCGTGGATAACGAGGACGGTGTGACGCCCAACGACTTTGACTTCTCCGTGTCCAACT TCGTGGACAACCTTTACGGCTACCCTGAAGGGAAAGACACTTTGCGGGAGACTATCAAGTTCATGTACAC AGACTGGGCCGATAAGGAAAACCCGGAGACGCGGCGGAAAACCCTGGTGGCTCTCTTTACTGACCATCAG TGGGTGGCCCCCGCCGTGGCCACCGCCGACCTGCACGCGCAGTACGGCTCCCCCACCTACTTCTATGCCT TCTATCATCACTGCCAAAGCGAAATGAAGCCCAGCTGGGCAGATTCGGCCCATGGCGATGAAGTCCCCTA TGTCTTCGGCATCCCCATGATCGGTCCCACAGAGCTCTTCAGTTGTAATTTCTCCAAGAACGACGTCATG CTCAGTGCCGTGGTGATGACCTACTGGACGAACTTCGCCAAAACTGGTGATCCAAACCAACCAGTTCCTC AGGATACCAAGTTCATTCATACAAAACCCAATCGCTTTGAAGAAGTGGCCTGGTCCAAGTATAATCCCAA AGACCAGCTCTATCTGCATATTGGCTTGAAACCCAGAGTGAGAGATCACTACCGGGCAACGAAAGTGGCT TTCTGGTTGGAATTGGTTCCTCATTTGCACAACTTGAACGAGATATTCCAGTATGTTTCAACAACCACAA AGGTTCCTCCACCAGACATGACATCATTTCCCTATGGCACCCGGCGATCTCCCGCCAAGATATGGCCAAC CACCAAACGCCCAGCAATCACTCCTGCCAACAATCCCAAACACTCTAAGGACCCTCACAAAACAGGGCCC GAGGACACAACTGTCCTCATTGAAACCAAACGAGATTATTCCACCGAATTAAGTGTCACCATTGCCGTCG GGGCGTCGCTCCTCTTCCTCAACATCTTAGCCTTTGCGGCGCTGTACTACAAAAAGGACAAGAGGCGCCA TGAGACTCACAGGCACCCCAGTCCCCAGAGAAACACCACAAATGATATCACTCACATCCAGAACGAAGAG ATCATGTCTCTGCAGATGAAGCAGCTGGAACACGATCACGAGTGTGAGTCGCTGCAGGCACACGACACGC TGAGGCTCACCTGCCCTCCAGACTACACCCTCACGCTGCGCCGGTCGCCGGATGACATCCCATTTATGAC GCCAAACACCATCACCATGATTCCAAACACATTGATGGGGATGCAGCCTTTACACACTTTTAAAACCTTC AGTGGAGGACAAAACAGTACAAATTTACCCCACGGACATTCCACCACTAGAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233056 representing NM_001206850 Red=Cloning site Green=Tags(s) MVYIHGGSYMEGTGNMIDGSILASYGNVIVITINYRLGILGFLSTGDQAAKGNYGLLDQIQALRWIEENV GAFGGDPKRVTIFGSGAGASCVSLLTLSHYSEGLFQKAIIQSGTALSSWAVNYQPAKYTRILADKVGCNM LDTTDMVECLKNKNYKELIQQTITPATYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLK FVDGIVDNEDGVTPNDFDFSVSNFVDNLYGYPEGKDTLRETIKFMYTDWADKENPETRRKTLVALFTDHQ WVAPAVATADLHAQYGSPTYFYAFYHHCQSEMKPSWADSAHGDEVPYVFGIPMIGPTELFSCNFSKNDVM LSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWSKYNPKDQLYLHIGLKPRVRDHYRATKVA FWLELVPHLHNLNEIFQYVSTTTKVPPPDMTSFPYGTRRSPAKIWPTTKRPAITPANNPKHSKDPHKTGP EDTTVLIETKRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHETHRHPSPQRNTTNDITHIQNEE IMSLQMKQLEHDHECESLQAHDTLRLTCPPDYTLTLRRSPDDIPFMTPNTITMIPNTLMGMQPLHTFKTF SGGQNSTNLPHGHSTTRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001206850
ORF Size	1944 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001206850.2
RefSeq Size	5079 bp
RefSeq ORF	1947 bp
Locus ID	22829
UniProt ID	Q8NFZ3
Protein Families	Druggable Genome, Transmembrane
MW	73.3 kDa
Gene Summary	This gene encodes a type I membrane protein that belongs to the family of neuroligins, which are cell adhesion molecules present at the postsynaptic side of the synapse, and may be essential for the formation of functional synapses. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Mar 2011]

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